Abstract
The production of α-amylase (α-1,4-glucan 4-glucanohydrolase; EC. 3.2.1.1) by a strain of Bacillus stearothermophilus isolated from leaf litter was investigated in a tryptone-maltose medium at 55°C in batch and chemostat culture. Amylase production was growth-limited and restricted to the exponential phase in batch culture. The enzyme yield was reduced by 40% when the culture pH was maintained at pH 7.2. Amylase production in chemostat culture was influenced by the growth rate throughout the dilution rate range used.
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Davis, P.E., Cohen, D.L. & Whitaker, A. The production of α-amylase in batch and chemostat culture by Bacillus stearothermophilus . Antonie van Leeuwenhoek 46, 391–398 (1980). https://doi.org/10.1007/BF00421985
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DOI: https://doi.org/10.1007/BF00421985