Summary
We have used cytoduction and protoplast fusion to characterise the killer trait in Torulopsis glabrata. Killer trait could not be transferred by cytoduction to a non-killer strain of T. glabrata suggesting that the determinant(s) is/are not present in the cytoplasm. Consistent with this interpretation is the observation that all fusion products, where chromosomes of both the parents are represented, are killers. Non-killer segregants could be isolated from fusion products after treatment with benlate suggesting that loss of the trait is associated with non-disjunction and loss of a chromosome.
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Sriprakash, K.S., Batum, C. Possible chromosomal location for the killer determinant in Torulopsis glabrata . Curr Genet 8, 115–119 (1984). https://doi.org/10.1007/BF00420229
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DOI: https://doi.org/10.1007/BF00420229