Summary
Transformants of sensitive yeast strains containing an expressed cDNA copy of the yeast killer toxin-immunity gene could be selected for by exposure to added killer toxin. For strain AH-22 the transformation frequency was approximately 10% that obtained by selection for leucine prototrophy. The procedure required time for expression of immunity prior to selection, and a screening step to remove non-transformed survivors. Under conditions where active toxin was produced, transformants containing the toxin-immunity gene were at a selective advantage, and cells losing the plasmid were killed. This resulted in self selection of transformants, and affords a way of maintaining plasmid stability in protrophic strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bostian KA, Elliot Q, Bussey H, Burn V, Smith A, Tipper DJ (1984) Cell 36:741–751
Brown AJP, Goodey AR, Tubb RS (1981) J Inst Brew (London) 87:234–238
Burrows S (1970) Baker's yeast. In: Rose AH, Harrison JS (eds) The yeasts, 3 Yeast technology. Academic Press, London, pp 349–420
Bussey H, Saville D, Hutchins K, Palfree RGE (1979) J Bacteriol 140:888–892
Harrison JS, Graham JCJ (1970) Yeasts in distillery practice. In: Rose AH, Harrison JS (eds) The yeasts, 3 Yeast technology. Academic Press, London, pp 283–348
Henderson RCA (1983) D-phil thesis. Botany School, Oxford University, England
Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Ito H, Fukuda Y, Musata K, Kimura A (1983) J Bacteriol 153:163–168
Kaster KR, Burgett SG, Ingolia TD (1984) Curr Genet 8:353–358
Kosaric N, Ng DCM, Russell I, Stewart GG (1980) Ethanol production by fermentation: an alternative liquid fuel. In: Perlman D (ed) Advances in applied microbiology 26. Academic Press, New York, pp 147–227
Lolle S, Skipper N, Bussey H, Thomas DY (1984) EMBO 3:1383–1387
Luria SE, Delbrück M (1943) Genetics 28:491–511
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning A Laboratory Manual. Cold Spring Harbor Laboratory, pp 86–91
Maule AP, Thomas PD (1973) J Inst Brew (London) 79:137–141
Southern EM (1975) J Mol Biol 98:503–517
Walmsley RM, Gardner DCJ, Oliver SG (1983) Mol Gen Genet 192:361–365
Webster TD, Dickson RC (1983) Gene 26:243–252
Young TW, Yagiu M (1978) Antonie van heeuwenhoek J Microbiol Serol 44:59–77
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bussey, H., Meaden, P. Selection and stability of yeast transformants expressing cDNA of an Ml killer toxin-immunity gene. Curr Genet 9, 285–291 (1985). https://doi.org/10.1007/BF00419957
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00419957