Skip to main content
SpringerLink
Log in

Transfer RNAs and tRNA genes of Vicia faba chloroplasts

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

Isolated chloroplasts from broad bean and common bean were found to contain a minimum of 31 and 32 tRNA species, respectively. These individual chloroplast tRNAs were 32P-labeled in vitro and hybridized to DNA fragments obtained upon digestion of broad bean and common bean chloroplast DNAs with various restriction endonucleases. At least 30 tRNA genes were localized on the physical maps of the two chloroplast genomes. Comparison of the broad bean tRNA gene map to that of common bean revealed DNA sequence rearrangements, such as inversions, insertions/ deletions and duplications, within these two members of the Legu minosae family.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bergmann P, Seyer P, Burkard G, Weil JH (1984) Plant Mol Biol 3:29–36

    Google Scholar 

  • Bohnert HJ, Crouse EJ (1981) Plant Mol Biol Newslett 2:70–72

    Google Scholar 

  • Bohnert HJ, Crouse EJ, Schmitt JM (1982) Organization and expression of plastid genomes. In: Parthier B, Boulter D (eds) 14B Encyclopedia of plant physiology. Springer, Berlin Heidelberg New York, p 475

    Google Scholar 

  • Bohnert HJ, Driesel AJ, Crouse EJ, Gordon K, Herrmann RG, Steinmetz A, Mubumbila M, Keller M, Burkard G, Weil JH (1979) FEBS Lett 103:52–56

    Google Scholar 

  • Bonnard G, Michel F, Weil JH, Steinmetz A (1984) Mol Gen Genet 194:330–336

    Google Scholar 

  • Bowman CM, Dyer TA (1979) Biochem J 183:605–613

    Google Scholar 

  • Buetow DE (1982) Molecular biology of chloroplasts. In: Govindjee (ed) II. Photosynthesis: development, carbon metabolism, and plant productivity. Academic Press, New York, p 43

    Google Scholar 

  • Burkard G, Steinmetz A, Keller M, Mubumbila M, Crouse EJ, Weil JH (1982) Resolution of chloroplast tranfer RNAs by two-dimensional gel electrophoresis. In: Edelman M, Hallick RB, Chua NH (eds) Methods in chloroplast molecular biology. Elsevier Biomedical Press, Amsterdam New York Oxford, p 347

    Google Scholar 

  • Chu NM, Tewari KK (1982) Mol Gen Genet 186:23–32

    Google Scholar 

  • Chu NM, Oishi K, Tewari KK (1981) Plasmid 6:279–292

    Google Scholar 

  • Crick FHC (1966) J Mol Biol 19:548–555

    Google Scholar 

  • Crouse EJ, Bohnert HJ, Schmitt JM (1984) Chloroplast RNA synthesis. In: Ellis RJ (ed) Chloroplast biogenesis, Seminar series of the society for experimental biology, vol 21. Univ Press, Cambridge (in press)

    Google Scholar 

  • Delius H, Koller B (1980) J Mol Biol 142:247–261

    Google Scholar 

  • Driesel AJ, Crouse EJ, Gordon K, Bohnert HJ, Herrmann RG, Steinmetz A, Mubumbila M, Keller M, Burkard G, Weil JH (1979) Gene 6:285–306

    Google Scholar 

  • Dyer TA, Bowman CM (1979) Biochem J 183:595–604

    Google Scholar 

  • Fluhr R, Edelman M (1981) Nucleic Acids Res 9:6841–6853

    Google Scholar 

  • Gordon KHJ, Crouse EJ, Bohnert HJ, Herrmann RG (1982) Theor Appl Genet 61:373–384

    Google Scholar 

  • Groot GSP, van Harten-Loosbroek N (1981) Curr Genet 4:187–190

    Google Scholar 

  • Ko K, Straus NA, Williams, JP (1983) Curr Genet 7:255–263

    Google Scholar 

  • Koller B, Delius H (1980) Mol Gen Genet 178:261–269

    Google Scholar 

  • Meeker R, Tewari KK (1980) Biochem (Wash) 19:5973–5981

    Google Scholar 

  • Meeker R, Tewari KK (1982) Biochim Biophys Acta 696:66–75

    Google Scholar 

  • Mubumbila M, Burkard G, Keller M, Steinmetz A, Crouse EJ, Weil JH (1980) Biochim Biophys Acta 609:31–39

    Google Scholar 

  • Mubumbila M, Gordon KHJ, Crouse EJ, Burkard G, Weil JH (1983) Gene 21:257–266

    Google Scholar 

  • Palmer JD (1982) Nucleic Acids Res 10:1593–1605

    Google Scholar 

  • Palmer JD (1983) Nature 301:92–93

    Google Scholar 

  • Palmer JD, Thompson WF (1981) Proc Natl Acad Sci USA 78:5533–5537

    Google Scholar 

  • Palmer JD, Thompson WF (1982) Cell 29:537–550

    Google Scholar 

  • Palmer JD, Singh GP, Pillay DTN (1983) Mol Gen Genet 190:13–19

    Google Scholar 

  • Rether B, Bonnet J, Ebel JP (1974) Eur J Biochem 50:281–288

    Google Scholar 

  • Selden RF, Steinmetz A, McIntosh L, Bogorad L, Burkard G, Mubumbila M, Kuntz M, Crouse EJ, Weil JH (1983) Plant Mol Biol 2:141–153

    Google Scholar 

  • Silberklang M, Gillum AM, RajBhandary UL (1977) Nucleic Acids Res 4:4091–4108

    Google Scholar 

  • Spielmann A, Ortiz W, Stutz F (1983) Mol Gen Genet 190:5–12

    Google Scholar 

  • Sugiura M (1980) Curr Genet 2:95–96

    Google Scholar 

  • Sun CR, Endo T, Kusuda M, Sugiura M (1982) Jpn J Genet 57:397–402

    Google Scholar 

  • Whitfeld PR, Bottomley W (1983) Ann Rev Plant Physiol 34:279–310

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut de Biologic Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, 15 rue Descartes, F-67084, Strasbourg Cedex, France

    M. Mubumbila, E. J. Crouse & J. H. Weil

Authors
  1. M. Mubumbila
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. J. Crouse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. H. Weil
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mubumbila, M., Crouse, E.J. & Weil, J.H. Transfer RNAs and tRNA genes of Vicia faba chloroplasts. Curr Genet 8, 379–385 (1984). https://doi.org/10.1007/BF00419827

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00419827

Key words

Search

Navigation