Summary
Use of a selective system for cytoduction in Saccharomyces cerevisiae allowed us to monitor hybrid formation and to clone the haploid nuclei of cells which have participated in illegitimate matings: a × a, α × α. Our approach has made it possible to select nuclei with mating-type switches and mutations within the MAT locus. It was shown that matings in α × α crosses often proceed through nonheritable genetic changes located within chromosome III. We suggest that these non-heritable genetic changes are due to premutational lesions, expressed phenotypically as transient α-matingtype. After a mating event these lesions are either repaired or converted to true mutations within the MAT locus.
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Repnevskaya, M.V., Karpova, T.S. & Inge-Vechtomov, S.G. Hybridization and cytoduction among yeast cells of the same mating type. Curr Genet 12, 511–517 (1987). https://doi.org/10.1007/BF00419560
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DOI: https://doi.org/10.1007/BF00419560