Summary
The synthesis of polypeptides related to the CPI chlorophyll-protein complex of photosystem I has been studied by pulse-labeling experiments in twenty chloroplast mutants of Chlamydomonas reinhardtii. Three mutations of the same locus (Girard-Bascou 1987) result in the absence of these CPI-related polypeptides. Among these mutations one, (FUD26) leads to the synthesis of a new polypeptide presumed to be a truncated CPI apoprotein. The molecular characterization of this mutation in the psaA2 gene has been achieved by DNA sequencing the 3′ end of this gene. The FUD26 mutation is a 4 base pair deletion resulting in frameshift and premature termination of the protein.
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References
Bennoun P, Girard J, Chua NH (1977) Mol Gen Genet 153: 343–348
Bennoun P, Diner B, Wollman FA, Schmidt GW, Chua NH (1981) Thylakoid polypeptides associated with photosystem II in Chlamydomonas reinhardtii comparison of system II mutants and particules. In: Akoyunoglou G (ed) Photosystem III, Structure and molecular organisation of the photosynthetic apparatus. Balaban international Science Services. Philadelphia. pp 839–849
Bennoun P, Spierer-Herz M, Erickson J, Girard-Bascou J, Pierre Y, Delosme M, Rochaix JD (1986) Plant Mol Biol 6:151–160
Berends T, Kubicek Q, Mullet J (1986) Plant Mol Biol 6:125–134
Birnboim HC, Dolly J (1979) Nucl Acid Res 7:1513–1523
Chua NH, Bennoun P (1975) Proc Natl Acad Sci USA 72: 2175–2179
Chua NH, Gillham NW (1977) J Cell Biol 74:441–452
Chua NH, Matlin K, Bennoun P (1975) J Cell Biol 67:361–377
Delepelaire P (1983) Photobiochem Photobiophys 6:279–292
Delepelaire P (1984) EMBO J 3:701–706
Delepelaire P, Chua NH (1979) Proc Natl Acad Sci USA 76: 111–115
Dron M, Rahire M, Rochaix JD (1982) J Mol Biol 162:775–793
Dron M, Rahire M, Rochaix JD, Mets LJ (1983) Plasmid 9: 321–324
Erickson J, Rahire M, Malnoe P, Girard-Bascou J, Pierre Y, Bennoun P, Rochaix JD (1986) EMBO J 5:1745–1754
Fish LE, Bogorad L (1986) J Biol Chem 261:8134–8139
Fish LE, Kuck U, Bogorad L (1985) J Biol Chem 260:1413–1421
Girard-Bascou J (1987) Curr Curr Genet 12:483–488
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Curr Genet 2:215–221
Gorman DS, Levine RP (1965) Proc Natl Acad Sci USA 54: 1665–1669
Grunstein M, Hogness D (1975) Proc Natl Acad Sci USA 72: 3961–3965
Hermann RG, Westhoff P, Alt J, Tittgen J, Nelson N (1985) In: van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Press. New York London, pp 233–256
Keegstra K, Cline K (1982) Plant Physiol 70:232–237
Kirsch W, Seyer P, Herrmann RG (1986) Curr Genet 10:843–855
Ko K, Strauss NA (1986) Plant Mol Biol 7:219–228
Kuck U, Choquet Y, Schneider M, Dron M, Bennoun P (1987) EMBO J 6:2185–2195
Laemmli UK (1970) Nature (London) 227:680–685
Lehmbeck J, Rasmussen OF, Bookjans GB, Jepsen BR, Stumman BM, Henningsen KW (1986) Plant Mol Biol 7:3–10
Lemaire C, Girard-Bascou J, Wollman FA, Bennoun P (1986) Biochim Biophys Acta 851:229–238
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Metz JG, Miles D (1982) Biochim Biophys Acta 681-95–102
Metz JG, Miles D, Rutherford AW (1983) Plant Physiol 73:452–459
Moller BL, Smilhe RM, Hóyer-Hansen G (1980) Carlsberg Res Commun 45:87–99
Nechustali R, Nelson N (1981) J Biol Chem 256:11624–11628
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574
Owens GC, Ohad I (1982) J Cell Biol 93:712–718
Piccioni RG, Bennoun P, Chua NH (1981) Eur J Biochem 117: 93–102
Rochaix JD (1978) J Mol Biol 126:597–617
Rochaix JD (1980) Methods Enzymol 65:785–795
Sanger F, Nichlen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schmidt GW, Bartlett SG, Grossman AR, Cashmore AR, Chua NH (1981) J Cell Biol 91:468–478
Shepherd HS, Boynton JE, Gillham NW (1979) Proc Natl Acad Sci USA 76:1353–1357
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamagichi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Vierling E, Alberte RS (1983) Plant Physiol 72:625–633
Westhoff P, Alt J, Nelson N, Bottomley W, Banemann H, Hermann RG (1983) Plant Mol Biol 2:95–107
Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Plant Mol Biol 3:177–190
Wollman FA, Bennoun P (1982) Biochim Biophys Acta 680: 352–360
Wollman FA (1986) Photosystem I proteins. In: Staehelin LA, Rantzen CJ (eds) Photosynthesis III: photosynthetic membranes and light harvesting systems. Springer, Berlin Heidelberg New York Tokyo, pp 487–495
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Girard-Bascoul, J., Choquet, Y., Schneider, M. et al. Characterization of a chloroplast mutation in the psaA2 gene of Chlamydomonas reinhardtii . Curr Genet 12, 489–495 (1987). https://doi.org/10.1007/BF00419557
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DOI: https://doi.org/10.1007/BF00419557