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The watermelon mitochondrial URF-1 gene: evidence for a complex structure

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Summary

We have cloned and sequenced a fragment of watermelon mitochondrial DNA (mtDNA) which contains a gene homologous to mitochondrial URF-1 (Unidentified Reading Frame-1) of vertebrates, Drosophila yakuba and Aspergillus nidulans. URF-1 is thought to encode a component of the respiratory chain NADH dehydrogenase. Two coding regions in the watermelon gene are separated by approximately 1,450 by of untranslatable DNA. These two exons encode the central portions of URF-1, and are highly conserved. We postulate that three additional exons, selected by their map location and amino acid homology to other URF-1 sequences, encode the remainder of the polypeptide. This is the first description of a plant mitochondrial gene with multiple introns.

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Author notes

  1. David B. Stern

    Present address: Department of Botany, University of California, 94720, Berkeley, CA, USA

  2. Anne G. Bang

    Present address: Max Planck Institut für Biochemie, D-8033, Martinsried, Germany

Authors and Affiliations

  1. Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, CA94305, Stanford, USA

    David B. Stern, Anne G. Bang & William F. Thompson

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  1. David B. Stern
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  2. Anne G. Bang
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  3. William F. Thompson
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Stern, D.B., Bang, A.G. & Thompson, W.F. The watermelon mitochondrial URF-1 gene: evidence for a complex structure. Curr Genet 10, 857–869 (1986). https://doi.org/10.1007/BF00418532

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  • DOI: https://doi.org/10.1007/BF00418532

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