Skip to main content
SpringerLink
Log in

Cloning and structure of a mouse interleukin-2 chromosomal gene

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Using non-stringent hybridization with a human interleukin-2 cDNA probe, we have isolated recombinant phages from a mouse genomic DNA library cloned in the EMBL3 phage. The sequence and organization of the mouse interleukin-2 (IL-2) gene was determined. By comparison with the human IL-2 sequence, three introns can be identified with lengths of 99, ±2 400, and ±1 900 base pairs, respectively. The mouse IL-2 gene codes for a polypeptide of 169 amino acids and contains a putative signal peptide of 20 amino acids. The homology to the human interleukin-2 is 72% at the nucleotide level in the coding part and 65% at the amino acid level. An extraordinary sequence, consisting of 12 consective CAG codons coding for glutamine, is found in the first exon.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Benton, W. D. & Davis, R. W., 1977. Science 196: 180–182.

    Google Scholar 

  2. Breathnach, R. & Chambon, P., 1981. Ann. Rev. Biochem. 50: 349–383.

    Google Scholar 

  3. Caplan, B., Gibbs, Cl. & Paetkau, V., 1981. J. Immunol. 126: 1351–1354.

    Google Scholar 

  4. Degrave, W., Tavernier, J., Duerinck, F., Plaetinck, G., Devos, R. & Fiers, W., 1983. EMBO J. 2: 2349–2353.

    Google Scholar 

  5. Devos, R., Plaetinck, G., Cheroutre, H., Simons, G., Degrave, W., Tavernier, J., Remaut, E. & Fiers, W., 1983. Nucl. Acids Res. 11: 4307–4323.

    Google Scholar 

  6. Frischauf, A.-M., Lehrach, H., Poustka, A. & Murray, N., 1983. J. Mol. Biol. 170: 827–842.

    Google Scholar 

  7. Fujita, T., Takaoka, Ch., Matsui, H. & Taniguchi, T., 1983. Proc. Natl. Acad. Sci. USA 80: 7437–7441.

    Google Scholar 

  8. Fuse, A., Fujita, T., Yasumitsu, H., Kashima, N., Hasegawa, K. & Taniguchi, T., 1984. Nucl. Acids Res. 12: 9323–9331.

    Google Scholar 

  9. Kashima, N., Nishi-Takoaka, C., Fujita, T., Taki, S., Yamada, G., Hamuro, J. & Taniguchi, T., 1985. Nature 313: 402–404.

    Google Scholar 

  10. Maxam, A. & Gilbert, W., 1980. In: Methods in Enzymology (Grossman, L. & Moldave, K. eds) vol. 65, pp. 499–560, Academic Press, New York.

    Google Scholar 

  11. Messing, J. & Vieira, J., 1982. Gene 19:269–276.

    Google Scholar 

  12. Morgan, D. A., Ruscetti, F. W. & Gallo, R. C., 1976. Science 193: 1007–1008.

    Google Scholar 

  13. Mount, S. M., 1982. Nucl. Acids Res. 10:459–472.

    Google Scholar 

  14. Robb, R. J., Kutny, R. M. & Chowdhry, V., 1983. Proc. Natl. Acad. Sci. USA 80:5990–5994.

    Google Scholar 

  15. Sabato, G. D., 1982. Proc. Natl. Acad. Sci. USA 79: 3020–3023.

    Google Scholar 

  16. Sanger, F., Nicklen, S. & Coulson, A. R., 1977. Proc. Natl. Acad. Sci. USA 74: 5463–5467.

    Google Scholar 

  17. Taniguchi, T., Matsui, H., Fujita, T., Takaoka, Ch., Kashima, N., Yoshimoto, R. & Hamuro, J., 1983. Nature 302: 305–310.

    Google Scholar 

  18. Tavernier, J., Derynck, R. & Fiers, W., 1981. Nucl. Acids Res. 9: 461–471.

    Google Scholar 

  19. Yokota, T., Arai, N., Lee, F., Rennick, D., Mosmann, T. & Arai, K.-I., 1985. Proc. Natl. Acad. Sci. USA 82: 68–72.

    Google Scholar 

  20. Wharton, K. A., Yedvobnich, B., Finnerty, V. G. & Artavanis Tsakosas, S., 1985. Cell 40: 55–62.

    Google Scholar 

Download references

Author information

Author notes

  1. G. Simons

    Present address: N.I.Z.O., Kernhemseweg 2, Postbus 20, 6710 BA, Ede, The Netherlands

Authors and Affiliations

  1. Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat 35, B-9000, Ghent, Belgium

    W. Degrave, G. Simons, R. Devos, G. Plaetinck, E. Remaut, J. Tavernier & W. Fiers

Authors
  1. W. Degrave
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Simons
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Devos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Plaetinck
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Remaut
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Tavernier
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W. Fiers
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Degrave, W., Simons, G., Devos, R. et al. Cloning and structure of a mouse interleukin-2 chromosomal gene. Mol Biol Rep 11, 57–61 (1986). https://doi.org/10.1007/BF00417597

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00417597

Keywords

Search

Navigation