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Arsenic elevates the sister chromatid exchange (SCE) rate in human lymphocytes in vitro

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Summary

The number of sister chromatid exchanges (SCE) per metaphase was determined in normal human lymphocytes by the Hoechst-Giemsa procedure. The addition of inorganic trivalent arsenic (Na2HAsO4) 10-6M and 2x10-6 M induces a significant and dose-dependent increase of SCE. This phenomenon is as sensitive as the evidence of arsenic-induced chromosomal aberrations in vitro.

Zusammenfassung

Die Zahl von »sister chromatid exchanges« (SCE) pro Metaphase wurde mit der Hoechst-Giemsa Technik an menschlichen Lymphocyten bestimmt. Die Zugabe von anorganischem trivalenten Arsen (Na2HAsO4) 10-6 M und 2x10-6 M zeigt einen signifikanten und dosisabhängigen Anstieg der SCE. Dieses Phänomen ist ebenso empfindlich wie der Nachweis von Chromosomenaberrationen der Lymphocyten durch Arsenexposition in vitro.

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References

  1. Baron, D., Kunick, I., Frischmuth, I., Petres, J.: Further in vitro studies on the biochemistry of the inhibition of nucleic acid and protein synthesis induced by arsenic. Arch Dermatol. Res. 253, 15–22 (1975)

    Google Scholar 

  2. Beckmann, G., Beckmann, L., Nordenson, I.: Chromosome aberrations in workers exposed to arsenic. Environ Health. Perspect. 19, 145–146 (1977)

    Google Scholar 

  3. Braun, W.: Krebs an Haut und inneren Organen hervorgerufen durch Arsen. Dtsch. Med. Wochenschr. 83, 870–872 (1959)

    Google Scholar 

  4. Burgdorf, W., Kurvink, K., Cervenka, J.: Elevated sister chromatid exchange rate in lymphocytes of subjects treated with arsenic. Human. Genet. 36, 69–72 (1977)

    Google Scholar 

  5. Degreef, H., Roelandts, R.: Arsenical intoxication due to pollution. Arch. Belgues Dermatol. 30, 35–38 (1974)

    Google Scholar 

  6. Denk, R., Holzmann, H., Lange, H.-J., Greve, D.: Über Arsenspätschäden bei obduzierten Moselwinzern. Med. Welt 20, 557–567 (1969)

    Google Scholar 

  7. Egyedi, K., Pataky, E.: Dermatologische Aspekte der Arsenintoxikation durch Trinkwasser. Dermatosen 26, 54–56 (1978)

    Google Scholar 

  8. Fierz, U.: Katamnestische Untersuchungen über die Nebenwirkungen der Therapie mit anorganischem Arsen bei Hautkrankheiten. Dermatologica (Basel) 131, 41–58 (1965)

    Google Scholar 

  9. Grobe, J. W.: Gutachterliche und therapeutische Befunde und Beobachtungen bei Moselwinzern mit Arsenintoxikationsspätfolgeschädigung. Berufsdermatosen 25, 124–130 (1977)

    Google Scholar 

  10. Jung, E. G., Trachsel, B.: Molekularbiologische Untersuchungen zur Arsencarcinogenese. Arch. Klin. Exp. Dermatol. 237, 819–826 (1970)

    Google Scholar 

  11. Nagy, G., Varga, G., Keresztes, T., Márfai, L.: Durch Genuß von hoch-arsenhaltigen Wasser verursachte chronische Arsenvergiftungen. Z. Hautkr. 50, 201–211 (1975)

    Google Scholar 

  12. Oppenheim, J. J., Fishbein, N. W.: Induction of chromosome breaks in cultured normal human leukocytes by potassium arsenite, hydroxyurea, and related compounds. Cancer Res. 25, 980–985 (1965)

    Google Scholar 

  13. Petres, J., Schmid-Ullrich, K., Wolf, U.: Chromosomenaberrationen an menschlichen Lymphozyten bei chronischen Arsenschäden. Dtsch. Med. Wochenschr. 95, 79–80 (1970)

    Google Scholar 

  14. Petres, J., Hundeiker, M.: »Chromosomenpulverisation« nach Arseneinwirkung auf Zellkulturen in vitro. Arch. Klin. Exp. Dermatol. 231, 366–370 (1968)

    Google Scholar 

  15. Petres, J., Berger, A.: Zum Einfluß anorganischen Arsens auf die DNS-Synthese menschlicher Lymphozyten in vitro. Arch. Dermatol. Forsch. 242, 343–352 (1972)

    Google Scholar 

  16. Petres, J., Baron, D., Hagedorn, M.: Effects of arsenic cell metabolism and cell proliferation: cytogenetic and biochemical studies. Environ. Health Perspect. 19, 223–227 (1977)

    Google Scholar 

  17. Reymann, F., Möller, R., Nielsen, A.: Relationship between arsenic intake and internal malignant neoplasms. Arch. Dermatol. (Chic.) 114, 378–381 (1978)

    Google Scholar 

  18. Schönwald, D., Passarge, E.: UV-light induced SCE in xeroderma pigmentosum lymphocytes. Hum. Gen. 36, 213–218 (1977)

    Google Scholar 

  19. Shu, Y.: Skin cancer in chronic arsenicism. Hum. Pathol. 4, 469–485 (1973)

    Google Scholar 

  20. Sommers, S. C., McManus, R. G.: Arsenical cancers of skin and internal organs. Cancer 6, 347–359 (1953)

    Google Scholar 

  21. Thiers, H., Colomb, D., Moulin, G., Colin, L.: Le Cancer Cutané arsenical des Viticulteurs du Beaujolais. Ann. Dermatol. Syph. (Paris) 94, 133–158 (1967)

    Google Scholar 

  22. Tseng, W. P., Chu, H. M., How, S. W., Fong, J. M., Lin, C. S., Shu, Y.: Prevalence of skin cancer in an endemic area of chronic arsenicism in Taiwan. J. Nat. Cancer Inst. 40, 453–463 (1968)

    Google Scholar 

  23. Wolf, R.: Zur Frage berufsbedingter Arsenschäden bei Winzern. Berufsdermatosen 22, 34–47 (1974)

    Google Scholar 

  24. Wolff, S.: Relation between DNS repair, chromosome aberrations, üand sister chromatid exchanges. In: DNA-repair mechanisms. Hanawalt, P. C., Friedberg, E. C., Fox, C. F. (eds.), pp. 751–760. Academic Press: 1978

  25. Wolff, S., Perry, P.: Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma 48, 341–353 (1974)

    Google Scholar 

  26. Wolff, S., Rodin, B., Cleaver, J. E.: Sister chromatid exchanges induced by mutagenic carcinogens in normal and xeroderma pigmentosum cells. Nature 265, 347–349 (1977)

    Google Scholar 

  27. Wolff-Schreiner, E., Carter, D. M., Schwarzacher, H. G., Wolff, K.: Sister chromatid exchanges in photochemotherapy. J. Invest. Dermatol. 69, 387–391 (1977)

    Google Scholar 

  28. Zanzoni, F., Baumann, J. W. A., Jung, E. G.: Sister chromatid exchange (SCE) in human lymphocytes. Effect of UVC irradiation and age. Arch. Dermatol. Res. 265, 283–287 (1979)

    Google Scholar 

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  1. Hautklinik der Städt. Krankenanstalten, Postfach 23, D-6800, Mannheim, Germany

    Flavia Zanzoni & E. G. Jung

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  1. Flavia Zanzoni
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  2. E. G. Jung
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Zanzoni, F., Jung, E.G. Arsenic elevates the sister chromatid exchange (SCE) rate in human lymphocytes in vitro. Arch Dermatol Res 267, 91–95 (1980). https://doi.org/10.1007/BF00416927

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  • DOI: https://doi.org/10.1007/BF00416927

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