Abstract
This study documents neuronal differentiation in vitro from undifferentiated precursor cells of caudalmost regenerating spinal cord of the teleost Apteronotus albifrons. At 11 days in vitro, cells from the caudalmost tip of the regenerating cord are flat and polygonal in shape, lack neuronal processes and do not stain with antibody against neuron-specific filaments. At 15 days in vitro, some of the caudalmost cells have developed short, neurite-like processes; at 18 days in vitro, some cells react positively with antibody against neuron-specific filaments. At 26 days in vitro, many of the caudalmost cells have long branching neurites and react positively with anti-neurofilament antibody. Addition of insulin-like growth factor-I to the medium accelerates the process of neuronal differentiation from the caudalmost precursor cells in vitro. The source of these precursor cells is ultimately cells of the ependymal layer of adult spinal cord. Further investigation of the factors that control production and differentiation of these cells will be important in defining the developmental potential possible for vertebrate spinal cord cells and may aid in creating an optimal environment for regeneration of axons within mammalian spinal cord.
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References
Anderson MJ (1993) Differences in growth of neurons from normal and regenerated spinal cord in vitro. In Vitro Cell Dev Biol 29:145–152
Anderson MJ, Waxman SG (1981) Morphology of regnerated spinal cord in Sternarchus albifrons. Cell Tissue Res 219:1–8
Anderson MJ, Waxman SG (1983a) Regeneration of spinal neurons in inframammalian vertebrates: morphological and developmental aspects. J Hirnforsch 24:371–398
Anderson MJ, Waxman SG (1983b) Caudal spinal cord of the teleost Sternarchus albifrons resembles regenerating cord. Anat Rec 205:85–92
Anderson MJ, Waxman SG (1985a) Neurogenesis in tissue cultures of adult teleost spinal cord. Dev Brain Res 20:203–212
Anderson MJ, Waxman SG (1985b) Neurogenesis in adult vertebrate spinal cord in situ and in vitro: a new model system. Ann NY Acad Sci 457:213–233
Anderson MJ, Waxman SG, Laufer M (1983) Fine structure of regenerated ependyma and spinal cord in Sternarchus albifrons. Anat Rec 205:73–83
Anderson MJ, Waxman SG, Tadlock CH (1984) Cell death of asynaptic neurons in regenerating spinal cord. Dev Biol 103:443–455
Anderson MJ, Choy CY, Waxman SG (1986) Selforganization of ependyma in regenerating teleost spinal cord: evidence from serial section reconstructions. J Embryol Exp Morphol 96:1–18
Bartlett PF, Reid HH, Bailey KA, Bernard O (1988) Immortalization of mouse neural precursor cells by the c-myc oncogene. Proc Natl Acad Sci USA 85:3255–3259
Caroni P, Grandes P (1990) Nerve sprouting in innervated adult skeletal muscle induced by exposure to elevated levels of insulin-like growth factors. J Cell Biol 110:1307–1317
Cattaneo E, McKay R (1990) Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature 347:762–765
DiCicco-Bloom E, Townes-Anderson E, Black IB (1990) Neuroblast mitosis in dissociated culture: regulation and relationship to differentiation. J Cell Biol 110:202–286
Drago J, Murphy M, Carroll SM, Harvey RP, Bartlett PF (1991) Fibroblast growth factor-mediated proliferation of central nervous system precursors depends on endogenous production of insulin-like growth factor 1. Proc Natl Acad Sci USA 88:2199–2203
Egar M, Simpson SB, Singer M (1970) The growth and differentiation of the regenerating spinal cord of the lizard, Anolis carolinensis. J Morphol 131:131–152
Frederiksen K, Jat PS, Valtz N, Levy D, McKay R (1988) Immortalization of precursor cells from the mammalian CNS. Neuron 1:439–448
Hammang JP, Baetge EE, Behringer RR, Brinster RL, Palmiter RD, Messing A (1990) Immortalized retinal neurons derived from SV40 T-antigen-induced tumors in transgenic mice. Neuron 4:775–782
Ishii D (1992) Neurobiology of insulin and insulin-like growth factors. In: Loughlin SE and Fallon JH (eds) Neurotrophic factors. Academic Press, New York, pp 415–442
Jacobson M (1991) Developmental Neurobiology, 3rd edn. Plenum Press, New York, pp 41–93
Koppanyi T (1955) Regeneration in the central nervous system of the fish. In: Windle WF (ed) Regeneration in the central nervous system. Thomas, Springfield Ill, pp 3–19
Letourneau PC (1975) Possible roles for cell-to-substratum adhesion in neuronal morphogenesis. Dev Biol 44:77–91
Mellon PL, Windle JJ, Goldsmith PC, Padula CA, Roberts JL, Weiner RI (1990) Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis. Neuron 5:1–10
Near S, Whalen LR, Miler JA, Ishii DN (1992) Insulin-like growth factor II stimulates motor nerve regeneration. Proc Natl Acad Sci USA 89:11716–11720
Peters A, Vaughn JE (1967) Microtubules and microfilaments in the axons and astrocytes of early postnatal optic nerves. J Biol Chem 32:113–120
Recio-Pinto E, Ishii D (1984) Effects of insulin, insulin-like growth factor-II and nerve growth factor on neurite outgrowth in cultured human neuroblastoma cells Brain Res 302:323–334
Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255:1707–1710
Reynolds BA, Tetzlaff W, Weiss S (1992) A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J Neurosci 11:4565–4574
Ronnett GV, Hester LD, Nye JS, Connors K, Snyder SH (1990) Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly. Science 248:603–604
Samson FE (1971) Mechanism of axoplasmic transport. J Neurobiol 2:347–353
Simpson SB (1964) Analysis of tail regeneration in the lizard Lygosoma laterale. I. Initiation of regeneration and cartilage differentiation: the role of ependyma. J Morphol 114:425–436
Sternberger LA (1986) Immunocytochemistry. Wiley, New York, pp 446–460
Sternberger LA, Harwell LW, Sternberger NH (1982) Neurotypy: regional individuality in rat brain detected by immunocytochemistry with monoclonal antibodies. Proc Natl Acad Sci USA 79:1326–1330
Waite JH, Tanzer ML (1981) Polyphenolic substance of Mytilus edulis: novel adhesive containing L-dopa and hydroxyproline. Science 212:1038–1040
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Anderson, M.J., Rossetto, D.L. & Lorenz, L.A. Neuronal differentiation in vitro from precursor cells of regenerating spinal cord of the adult teleost Apteronotus albifrons . Cell Tissue Res. 278, 243–248 (1994). https://doi.org/10.1007/BF00414166
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DOI: https://doi.org/10.1007/BF00414166