Abstract
Superoxide dismutase activity was demonstrated for 6 strains of 3 propionibacteria species. Rather high level of superoxide dismutase activity found in propionibacteria was in accordance with high level of catalase activity reported for propionibacteria previously. Both these activities were shown to have cytozolic localization. For the first time peroxidase activity was detected in gel-fractionated crude cell extracts of propionibacteria. The ability to produce superoxide radicals in NADH-dependent oxidation system was revealed for three strains of the bacteria. The level of superoxide production by the membrane particles of the propionic acid bacteria correlated with the levels of superoxide dismutase and catalase activities and was the lowest for Propionibacterium shermanii. The ability to perform monovalent oxygen reduction during succinate oxidation was not revealed. The intact cells of P. globosum, P. vannielii, P. shermanii apparently did not excrete superoxide radicals into culture fluid during respiration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Bonartseva GA, Taptykova SD, Vorobyova LI, Krainova OA, Bryukhacheva NL (1973) The aerobic metabolism of propionic acid bacteria. Mikrobiologiya 42:765–771
Boveris A, Cadenas E, Stoppani A (1976) Role of ubiquinone in the mitochondrial generation of hydrogen peroxide. Biochem J 156:435–444
Britton L, Fridovich I (1977) Intracellular localization of superoxide dismutase of E. coli: a reevaluation. J Bacteriol 131:815–820
Bryukhacheva NL, Bonartseva GA, Vorobyova LI (1975) Oxidative phosphorylation in propionic acid bacteria. Mikrobiologiya 44:11–15
Delwiche EA, Carson SF (1953) A citric acid cycle in Propionibacterium pentosaceum. J Bacteriol 65:318–321
Fridovich I (1978) The biology of oxygen radicals. Science 201:875–880
Gregory EM, Fridovich I (1974) Visualization of catalase on acrylamide gels. Anal Biochem 58:57–62
Herzog V, Fahimi HD (1973) A new sensitive colorimetric assay for peroxidase using 3,3-diaminobenzidene as hydrogen donor. Anal Biochem 55:554–562
Kraeva NI, Vorobyova LI (1981) Intracellular localization, isolation and characterization of superoxide dismutase from Propionibacterium globosum. Prikladnaya biokhimiya and mikrobiologiya 6:837–842
Laemmli UK (1970) The cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:681–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
McCord JM, Fridovich I (1969) Superoxide dismutase: an enzymatic function for erythrocuprein. J Biol Chem 244:6049–6055
Meier B, Schwartz AC (1978) Superoxide dismutase and anaerobiosis of propionic acid bacteria. Hoppe-Seyler's Z Physiol Chem 359:1121–1122
Misra HP, Fridovich I (1972) The role of superoxide anions in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175
Pritchard GG, Wimpenny JWT, Morris HA, Lewis MWA, Hughes DE (1977) Effects of oxygen on Propionibacterium shermanii grown in continuous culture. J Gen Microbiol 102:223–233
Privalle CT, Gregory EM (1979) Superoxide dismutase and O2 lethality in Bacteroides fragilis. J Bacteriol 138:139–145
Schwartz AC, Sporkenbach J (1975) The electron transport system of the anaerobic Propionibacterium shermanii. Cytochrome and inhibitor studies. Arch Microbiol 102:261–273
Sherman JM (1921) The cause of eyes and characteristic flavor in Emmental or Swiss cheese. J Bacteriol 6:379–392
Shvinka UE, Viestur UE, Toma MK (1979) Alternative pathways of oxidation in the respiration chain of Brevibacterium flavum. Mikrobiologiya 48:10–16
Simon A (1968) Einfluß einiger Faktoren auf die Produktion von Vitamin B12 und die Katalaseaktivität von Kulturen des Propionibacterium shermanii. Zbl Bakteriol, Parasitkde Infektionskrankh Hyg 122:143–154
Sone N (1972) The redox reactions in propionic acid fermentation. I. Occurrence and nature of an electron transfer system in Propionibacterium arabinosum. J Biochem 71:931–940
Vorobyova LI (1976) Propionic acid bacteria and vitamin B12 formation. Edition of Moscow State University, Moscow
Vorobyova LI, Golozubova GA, Safonov VI (1968) Catalase of propionic acid bacteria. Prikladnaya biokhimiya and mikrobiologiya 4:654–658
Vries W de, Wyck-Kapteyn WMC van, Stouthamer AH (1972) Influence of oxygen on growth, cytochrome synthesis and fermentation pattern in propionic acid bacteria. J Gen Microbiol 71:515–524
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vorobyova, L.I., Kraeva, N.I. Superoxide radicals and antiradical defence of propionic acid bacteria. Arch. Microbiol. 133, 110–113 (1982). https://doi.org/10.1007/BF00413521
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00413521