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Immunohistochemical demonstration of MAM-3 and MAM-6 antigens in normal human skin appendages and their tumors

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Summary

The expression of MAM-3 and MAM-6 antigens was immunohistochemically investigated on 110 tumors of human skin appendages. Forty-two samples from tumor-adjacent normal skin appendages were also studied. MAM-3 antigens, as detectable by monoclonal antibodies (MoAbs) 67D11, 115G3, and 115H10 were present in the inner layer cells but not in the outer layer cells of normal eccrine excretory ducts. Sporadic positivity was also found in cytoplasm of apocrine acini with 115G3, while 67D11 and 115H10 were negative. MAM-6 antigens, as detectable by the MoAbs 115D8, 115F5, 139H2, 140C1, and 126E7 were found in the secretory canaliculi of normal eccrine acini and within the apical lumina at the terminal portion of ducts. Apocrine acinar cells mainly exhibited an apical staining, but a focal supranuclear dot-like staining could also be observed. A foamy reaction pattern for MAM-6 was noted in mature sebocytes. However, none of the antigenic epitopes was expressed in normal squamous epithelium or hair follicles. In benign tumors, the staining patterns for both antigens, in general, resembled their distribution in the corresponding normal tissues. However, carcinomas orginating from sweat glands, sebaceous glands, and the pilar apparatus expressed both antigens in a more irregular and heterogeneous pattern. This might preferably be explained by the loss of those mechanisms controlling the antigen expression in mature, functional tissues. Conclusions from these immunohistochemical studies with regard to the histogenesis mainly of the malignant skin appendage tumors should be drawn with caution.

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References

  1. Arends JW, Wiggers T, Verstijnen C, Bosman FT, Swaen GJV, Hilgers J, Lennox E, Steplewski Z (1984) Tumor cell heterogeneity in primary and metastatic colorectal carcinoma. Prot Biol Fluids 31:578–594

    Google Scholar 

  2. Cotton DWK, Braye SG (1984) Dermal cylindromas originate from the eccrine sweat gland. Br J Dermatol 111: 53–61

    Google Scholar 

  3. Egan MJ, Crocker J, Newman J, Collard M (1986) Immunohistochemical localization of S100 protein in skin tumors. Arch Pathol Lab Med 110:765–767

    Google Scholar 

  4. Foster CS, Dinsdale EA, Edwards PAW, Neville AM (1982) Monoclonal antibodies to the human mammary gland. I. Distribution of determinants in non-neoplastic mammary. and extra mammary tissue. Virchow Arch (Pathol Anat) 394:279–293

    Google Scholar 

  5. Hageman Ph, Van den Tweel J, Hilgers J, Hilkens J, Peterse H, Delemarre J, Van der Valk M, Van Doornewaard G, Atsma D, Schol D (1984) Sweat glands and salivary glands as model system for the characterization of monoclonal antibodies against differentiation antigens of the human mammary gland. Prot Biol Fluids 31:1009–1012

    Google Scholar 

  6. Hashimoto K, Lever WF (1964) Eccrine poroma; histochemical and electron mioroscopic studies. J Invest Dermatol 43:237–247

    Google Scholar 

  7. Hashimoto K, Gross BG, Lever WF (1966) Syringoma; histochemical and electron microscopic studies. J Invest Dermatol 46:150–166

    Google Scholar 

  8. Hashimoto K, Gross BG, Nelson RG, Lever WF (1966) Eccrine spiradenoma; histochemical and electron microscopic studies. J Invest Dermatol 46:347–365

    Google Scholar 

  9. Headington UT (1961) Mixed, tumors of the skin; eccrine and apocrine types. Arch Dermatol 84:986–996

    Google Scholar 

  10. Hilkens J, Buijs F, Hilgers J, Hageman Ph, Calafat J, Sonnenberg A, Van der Valk A (1984) Monoclonal antibodies against human milk fat globule membranes detecting differentiation antigens of the mammary gland and its tumors. Int J Cancer 34:197–206

    Google Scholar 

  11. Hsu SM, Raine L, Fanger H (1981) Use of avindin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    Google Scholar 

  12. Kanitakis U, Zambruno G, Viac J, Thivolet J (1986) Involcurin expression in adnexal skin tumors. An immunohistochemical study. Virchow Arch (Pathol Anat) 408:527–540

    Google Scholar 

  13. Kariniemi Al, Forsman LM, Wahlström T, Anderson LC (1984) Expression of differentiation antigens in benign sweat gland tumors. Br J Dermatol 111:175–182

    Google Scholar 

  14. Koldovsky U, Wargalla U, Hilkens J, Taylor-Papadimitriou J, Hageman Ph, Hilgers J (1984) Reactions of monoclonal antibodies against human milk fat globule membranes with embryonal tissue. Prot Biol Fluids 31:1021–1024

    Google Scholar 

  15. Mariani-Constantini R, Colnaghi MI, Leoni F, Menard S, Cerasoli S, Rilke F (1984) Immunohistochemical reactivity of monoclonal antibody prepared against human breast, carcinoma. Virchow Arch (Pathol Anat) 402:389–404

    Google Scholar 

  16. Mazoujian G, Pinkus GS, Davis S, Haagensen DE (1983) Immunohistochemistry of a gross cystic disease protein (GCDPF-15) of the breast. A marker of apocrine epithelium and breast carcinoma with apocrine features. Am J Pathol 110:105–112

    Google Scholar 

  17. Menard S, Tagliabuee E, Canevari S, Fossati G, Colnaghi MI (1983) Generation of monoclonal antibodies reacting with normal and cancer cells of human breast. Cancer Res 43:1295–1300

    Google Scholar 

  18. Noda Y, Takai Y, Iwai Y, Meenaghan MA, Mori M (1986) Immunohistochemical study of carbonic anhydrase in mixed tumors from major salivary glands and skin. Virchow Arch (Pathol Anat) 408:449–459

    Google Scholar 

  19. Pennys NS, Nadji M, McKinney EC (1981) Carcinoembryonic antigen is present in human eccrine sweat gland. J Am Acad Dermatol 4:401–403

    Google Scholar 

  20. Pennys NS, Nadji M, Morales A (1982) Carcinoembryonic antigen in benign sweat gland tumors. Arch Dermatol 118:225–227

    Google Scholar 

  21. Pennys NS, Nadji M, Ziegel-Weissman J, Ketabchi M, Morales AR (1982) Carcinoembryonic antigen in sweat gland carcinomas. Cancer 50:1608–1611

    Google Scholar 

  22. Rasmussen BB, Hilgers J, Hilkens J (1984) The influence of formalin and paraffin embdded antibodies applied to female breast tissue. Acta Pathol Microbiol Scand See A 92:167–175

    Google Scholar 

  23. Tsubura A, Morii S, Hilkens J, Hilgers J (1985) Expression of MAM-3 and MAM-6 antigens on endometrial and endocervical cancers. Virchow Arch (Pathol Anat) 407: 59–67

    Google Scholar 

  24. Wagenaar Sj Sc, Hilgers J, Schmitz du Moulin F, Schol D, Verstijnen C, Hilkens J, Osanto S, Vanderscheuren R (1984) Patterns of expression of some new antigens of human bronchial carcinomas. Prot Biol Fluids 31:521–524

    Google Scholar 

  25. Zotter S, Lossnitzer A, Kunze K-D, Muller M, Hilkens J, Hilgers J, Hageman PH (1985) Epithelial markers for paraffin-embedded human tissues; immunohistochemistry with monoclonal antibodies against milk fat globule antigens. Virchow Arch (Pathol Anat) 406:237–251

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Kansai Medical University, 570, Moriguchi, Osaka, Japan

    A. Tsubura & S. Morii

  2. Department of Pathology, Osaka Red Cross Hospital, 553, Osaka, Osaka, Japan

    S. Ueda & M. Sasaki

  3. Institute of Pathological Anatomy, Medical Academy, 8019, Dresden, DDR

    St. Zotter

  4. Clinic of Dermatology, University Jena, 6900, Jena, DDR

    V. Watzig

  5. Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

    W. Mooi, PhC. Hageman, J. Hilkens & J. Hilgers

  6. Department of Pathology, AMC, Meibergdeef, 15, 1105 AZ, Amsterdam, The Netherlands

    J. Van den Tweel

  7. Department of Pathology, Free University, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands

    C. Meijer

Authors
  1. A. Tsubura
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  2. S. Morii
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  3. S. Ueda
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  4. M. Sasaki
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  5. St. Zotter
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  6. V. Watzig
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  7. W. Mooi
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  8. PhC. Hageman
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  9. J. Hilkens
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  10. J. Van den Tweel
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  11. C. Meijer
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  12. J. Hilgers
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Tsubura, A., Morii, S., Ueda, S. et al. Immunohistochemical demonstration of MAM-3 and MAM-6 antigens in normal human skin appendages and their tumors. Arch Dermatol Res 279, 550–557 (1987). https://doi.org/10.1007/BF00413288

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  • DOI: https://doi.org/10.1007/BF00413288

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