Abstract
Adenylylsulphate kinase (EC 2.7.1.25, ATP:adenylylsulphate 3′-phosphotransferase) has been isolated from Escherichia coli and from Saccharomyces cerevisiae. As major steps of purification, affinity chromatography on Sepharose CL 6B (“blue” or “red”) and chromatofocusing on polybuffer PBE 94tm were employed. The proteins were obtained in nearly homogeneous state after five chromatographic steps.
The isolated enzymes from both sources appeared predominantly to exist as dimers. Upon reduction of the protein with dithiothreitol, it desintegrated into assumingly identical smaller subunits (E. coli rom Mr 90-85000 to 45-40000 and s. cerevisiae from 52-49500 to 28-29500). Both forms, dimer and monomer were found catalytically active.
Preincubation of the isolated enzyme from either source in the presence of thioredoxin plus DTT, reduced glutathione or DTT increased the activity significantly. Treatment of the enzyme with SH-blocking reagents inactivated the enzyme irreversibly as compared to the inactivation caused by oxidants (2,6-dichlorophenol-indophenol, ferricyanide or oxydized glutathione). This oxidant induced inactivation was less pronounced for the fungal enzyme than for the bacterial protein. The enzyme from E. coli required thioredoxin in order to alleviate the GSSG-induced inactivation.
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Abbreviations
- APS:
-
adenylylsulphate
- APS:
-
kinase
- ATP:
-
adenylylsulphate 3′-phosphotransferase
- DCPIP:
-
2,6-dichlorophenol indophenol
- DTT:
-
dithiothreitol
- GSH:
-
reduced glutathione
- GSSG:
-
oxidized glutathione
- HPLC:
-
high performance liquid chromatography
- β-MSH:
-
β-mercaptoethanol
- PAPS:
-
3′-phosphoadenylylsulphate
- TNBS:
-
2,4,6 tri-nitrobenzenesulphonic acid
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schriek, U., Schwenn, J.D. Properties of the purified APS-kinase from Escherichia coli and Saccharomyces cerevisiae . Arch. Microbiol. 145, 32–38 (1986). https://doi.org/10.1007/BF00413024
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DOI: https://doi.org/10.1007/BF00413024