Skip to main content
SpringerLink
Log in

Diuresis induced by water infusion into the carotid loop of unanaesthetized dogs

  • Published:
Pflüger's Archiv für die gesamte Physiologie des Menschen und der Tiere Aims and scope Submit manuscript

Zusammenfassung

An fünf wachen, trainierten Hunden mit Carotisschlinge wurde die Wirkung kleiner intracarotidialer Wasserinfusionen auf die Harnausscheidung untersucht. Die Infusionsgeschwindigkeiten betrugen 3,0, 2,5 und 2,0 ml/min. Während einer Infusionszeit von 30 min wurden also insgesamt 90, 75 bzw. 60 ml Wasser injiziert. Als Kontrollversuch dienten entsprechende Infusionen in eine periphere Vene.

Eine Diurese trat nur mit intracarotidialer Infusion ein. Dies spricht zugunsten einer zentralen Lokalisation der verantowrtlichen Osmoreceptoren. Die Größe der Diurese war eine Funktion der infundierten Wassermenge. Sie beruhte ausschließlich auf einer Erhöhung der Freiwasserclearance. Die Exkretion der Elektrolyte und die osmotische Clearance blieben unverändert. Diese Befunde können am besten über den ADH-Mechanismus erklärt werden.

Summary

In five unanaesthetized trained dogs the effect of injection of small quantities of water into a carotid loop on the excretion of urine was investigated. The infusion rates were 3.0, 2.5 and 2.0 ml/min. Infusion time was 30 min, so that a total of 90, 75 and 60 ml of water was injected. As controls, identical water infusions were made into a peripheral vein. The following results were obtained:

Diuresis occurred only with intracarotid infusions. This speaks in favour of a central location of osmoreceptors. The diuresis was a function of the amount of water infused and was due solely to an increase in free water clearance. The excretion of electrolytes and the osmotic clearance remained unchanged. These findings can best be explained by the ADH mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Ames, R. G., D. H. Moore, and H. B. van Dyke: The excretion of posterior pituitary antidiuretic hormone in the urine and its detection in the blood. Endocrinology 46, 215 (1950).

    Google Scholar 

  2. Anderson, B.: The effect of injections of hypertonic NaCl-solutions into different parts of the hypothalamus of goats. Acta physiol. scand. 28, 188 (1953).

    Google Scholar 

  3. ——, and S. M. McCann: A further study of polydipsia evoked by the hypothalamic stimulation in the goat. Acta physiol. scand. 33, 333 (1955).

    Google Scholar 

  4. Arndt, J. O.: Diuresis induced by water infusion into the carotid loop and its inhibition by small hemorrhage. The competition of volume- and osmocontrol. Pflügers Arch. ges. Physiol. 282, 313 (1965).

    Google Scholar 

  5. Cow, D.: Einige Studien zur Diurese. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 69, 393 (1912).

    Google Scholar 

  6. Cross, B. A., and J. D. Green: Activity of single neurones in the hypothalamus: Effect of osmotic and other stimuli. J. Physiol. (Lond.) 148, 554 (1959).

    Google Scholar 

  7. Euler, C. von: A preliminary note on slow hypothalmic “Osmopotentials”. Acta physiol. scand. 29, 133 (1953).

    Google Scholar 

  8. Fang, H. S., H. M. Liu, and S. C. Wang: Liberation of antidiuretic hormone following hypothalamic stimulation in the dog. Amer. J. Physiol. 202, 212 (1962).

    Google Scholar 

  9. Friedman, S. M., J. A. Hinke, and C. L. Friedman: Neurohypophyseal responsiveness in the normal and sensecent rat. J. Geront. 11, 286 (1956).

    Google Scholar 

  10. Gauer, O. H., u. J. P. Henry: Beitrag zur Homöostase des extraarteriellen Kreislaufs. Klin. Wschr. 34, 356 (1956).

    Google Scholar 

  11. —— —— Circulatory basis of fluid volume control. Physiol. Rev. 43, 423 (1963).

    Google Scholar 

  12. Ginsberg, W.: Diureseversuche. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 69, 381 (1912).

    Google Scholar 

  13. Grossman, J.: Volume factors in body fluid regulation. Arch. intern. Med. 99, 93 (1957).

    Google Scholar 

  14. Haberich, F. J., O. Aziz u. P. E. Nowacki: Über das unterschiedliche Verhalten der Diurese bei intraportalen und intracavalen Infusionen von Wasser und 0,9% NaCl-Lösung an der wachen Ratte. Pflügers Arch. ges. Physiol. 279, R 30 (1964).

  15. Haldane, J. S.: The regulation of excretion of water by the kidney. J. Physiol. (Lond.) 50, 297 (1916).

    Google Scholar 

  16. Holland, R. C., J. W. Sundsten, and C. H. Sawyer: Effects of intracarotid injections of hypertonic solutions on arterial pressure in the rabbit. Circulat. Res. 7, 712 (1959).

    Google Scholar 

  17. Hoppe-Seyler/Thierfelder: Handbuch der physiologisch- und pathologisch-chemischen Analyse. Bd. 2, S. 29–32. 10. Aufl. Berlin, Göttingen, Heidelberg: Springer 1955. (Schatzmann, H. J.: Osmotischer Druck. b) Methoden zur Bestimmung des osmotischen Druckes oder des Molekulargewichtes mit Anwendungsmöglichkeiten in der Biologie. a) Die Gefrierpunktbestimmung.)

    Google Scholar 

  18. Jewell, P. A., and E. B. Verney: An experimental attempt to determine the site of neurohypophyseal osmoreceptors in the dog. Phil. Trans. B 240, 197 (1957).

    Google Scholar 

  19. Lasser, R. P., M. R. Schoenfeld, D. F. Allen, and C. K. Friedberg: Reflex circulatory effects elicited by hypertonic and hypotonic solutions injected into femoral and brachial arteries of dogs. Circulat. Res. 8, 913 (1960).

    Google Scholar 

  20. Leaf, A., and H. S. Frazer: Some recent studies on the actions of neurohypophyseal hormones. Progr. cardiovasc. Dis. 1, 47 (1961).

    Google Scholar 

  21. ——, and A. R. Mamby: The normal antidiuretic mechanism in man and dog; its regulation by extracellular fluid tonicity. J. clin. Invest. 31, 54 (1952).

    Google Scholar 

  22. van Leersum, E. C.: Eine Methode zur Erleichterung der Blutdruckmessung am Hunde. Pflügers Arch. ges. Physiol. 142, 377 (1911).

    Google Scholar 

  23. McCance, R. A.: Experimental sodium chloride deficiency in man. Proc. roy. Soc. B 119, 245 (1936).

    Google Scholar 

  24. Millies, E.: A new diuretic factor of hepatic origin. Acta physiol. lat. amer. 10, 178 (1960).

    Google Scholar 

  25. O'Connor, W. J.: Renal Function. London: Arnold 1962.

    Google Scholar 

  26. Pabst, K., u. O. H. Gauer: Nierenhämodynamik und Ausscheidungsfunktion der Niere unter definierten Änderungen des Blutvolumens. Pflügers Arch. ges. Physiol. 274, 213 (1961).

    Google Scholar 

  27. Priestley, I. G.: The regulation of excretion of water by the kidney. J. Physiol. (Lond.) 50, 304 (1916).

    Google Scholar 

  28. Rein, H.: Über die Besonderheiten der Blutzirkulation in der Arteria carotis. Z. Biol. 89, 307 (1930).

    Google Scholar 

  29. Sawyer, C. H., and B. E. Gernandt: Effects of intracarotid and intraventricular injections of hypertonic solutions on electrical activity of rabbit brain. Amer. J. Physiol. 185, 209 (1956).

    Google Scholar 

  30. Smith, H. W.: Principles of Renal Physiology. New York: Oxford University Press 1960.

    Google Scholar 

  31. —— Salt and water volume receptors. Amer. J. Med. 23, 623 (1957).

    Google Scholar 

  32. Verney, E. B.: The antidiuretic hormone and the factors which determine its release. Proc. roy. Soc. B 135, 25 (1947).

    Google Scholar 

  33. —— Osmoregulation und antidiuretisches Hormon. Ärztl. Wschr. 13, 1010 (1958).

    Google Scholar 

  34. Wesson, L. G., jr.: Electrolyte excretion studies in the dog. Meth. med. Res. 5, 175 (1952).

    Google Scholar 

  35. Zuidema, G. D., and N. P. Clarke: Central localization of the osmotic control center. Amer. J. Physiol. 188, 616 (1956).

    Google Scholar 

  36. —— —— and M. T. Minton: Osmotic regulation of body fluids. Amer. J. Physiol. 187, 85 (1956).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. W. G. Kerckhoff-Institut der Max Planck-Gesellschaft, Bad Nauheim

    Joachim O. Arndt & Otto H. Gauer

  2. Physiologisches Institut der Freien Universität Berlin, Germany

    Joachim O. Arndt & Otto H. Gauer

  3. School of Medicine, Department of Physiology and Biophysics, University of Washington, Seattle, Washington

    Joachim O. Arndt

Authors
  1. Joachim O. Arndt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Otto H. Gauer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

To Professor Max Schneider on his 60th birthday.

With 4 Figures in the Text

A preliminary report of this work was given at the 28th meeting of the Deutsche Physiologische Gesellschaft [Pflügers Arch. ges. Physiol. 278, 51 (1963)].

This research was conducted under the sponsorship of the Deutsche Forschungsgemeinschaft and Grant No. AF EOAR 62–75 U.S. Airforce, Brussels Office.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arndt, J.O., Gauer, O.H. Diuresis induced by water infusion into the carotid loop of unanaesthetized dogs. Pflügers Arch. 282, 301–312 (1965). https://doi.org/10.1007/BF00412505

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00412505

Keywords

Search

Navigation