Summary
The cellulase complex of Trichoderma koningii has been separated into four apparently pure components namely cellobiase, a C1-like component and two new components, one a CM-cellulase, the other named component C2. All four are necessary for efficient solubilisation of native cellulose. 2. The two new components together constitute the composite CM-cellulase-short-fibre forming activity described by Halliwell and Riaz (1970). 3. Alone only components C2 and C1 have any action on the substrate, the former being somewhat more effective than the C1 component which shows weak solubilising power. 4. Component C2 degrades cellulose weakly to short-fibres but synergises extensively with the CM-cellulase in promoting this process. In contrast the CM-cellula se fails to react significantly with component C1. 6. The interaction of all four components in contributing to the degradation of native cellulose is discussed.
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Halliwell, G., Riaz, M. Interactions between components of the cellulase complex of Trichoderma koningii on native substrates. Archiv. Mikrobiol. 78, 295–309 (1971). https://doi.org/10.1007/BF00412270
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DOI: https://doi.org/10.1007/BF00412270