Summary
The Stickland reaction was studied in suspensions of starved cells of Clostridium sticklandii with the aid of NADH fluorescence, and enzymic determinations of NADH.
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1.
Cells of C. sticklandii can be starved by anaerobic incubation with a hydrogen-accepting substrate, such as glycine or proline.
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2.
Arginine or formate, known reductants of C. sticklandii cause a rapid reduction of NAD in starved cells, which is reversed upon the addition of oxidants, such as glycine or proline.
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3.
New reductants in the Stickland reaction of this organism are alanine, serine, and threonine, as they cause a marked increase of NADH fluorescence in starved cells.
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4.
New oxidants are glutamate, histidine, adenine, uracil, thymine, uric acid, and xanthine, as established by the reverse criterion, the decrease of the NADH fluorescence of starved cells previously reduced with an optimal reductant.
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Abbreviations
- C. :
-
Clostridium
- wt.:
-
weight
References
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Schwartz, A.C., Schäfer, R. New amino acids, and heterocyclic compounds participating in the stickland reaction of Clostridium sticklandii . Archiv. Mikrobiol. 93, 267–276 (1973). https://doi.org/10.1007/BF00412026
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DOI: https://doi.org/10.1007/BF00412026