Summary
Enzyme activities have been measured and compared at several stages of the development of Neurospora crassa i.e. from free conidia (inoculum) to conidiated mycelia grown on sucrose versus acetate (poor versus highly conidiogenous) media.
Glucose-6-phosphate dehydrogenase (G-6-PDH) and NADP nucleotidase (NADPase) show inverse activity-time curves, both on sucrose and acetate media. G-6-PDH has its higher activity at the preconidiating stage while NADPase progressively increases to reach its maximal value in the mature conidia.
Malate dehydrogenase (MDH) has a higher activity in extracts from acetate compared to sucrose cultures; in both conditions, maximal MDH activity corresponds with the initiation of conidiation. Malate synthetase (MS) has a delayed activity which is much higher in acetate extracts.
Succinate dehydrogenase (SDH) is more active in sucrose than acetate extract with a sharp activity peak just preceding conidiation proper.
Isozymes of G-6-PDH and MDH, as well as total soluble proteins from extracts of sucrose versus acetate cultures have been compared after their separation on polyacrylamide columns.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Références
Bergmeyer, H. U.: Glucose-6-phosphate dehydrogenase. Methods of enzymatic analysis, pp. 744–751 New York-London: Acad. Press 1965.
Bianchi, D. E., Turian, G.: Nuclear division in Neurospora crassa during conidiation and germination. Experientia (Basel) 23, 192 (1967).
Combépine, G.: Etude comparative de la transaminase alanine-glyoxylate et malate-synthétase chez Neurospora crassa. Bull. Soc. Chim. biol. (Paris) 51, 1309–1317 (1969).
— Chervet, J.: Preparation of isolated macroconidia of Neurospora crassa. Experientia (Basel) 25, 984 (1969).
— Turian, G.: Recherches sur la biosynthèse de la glycine chez Neurospora type sauvage et mutants. Path. et. Microbiol. (Basel) 28, 1018–1030 (1965).
—— Métabolisme de la glycine et conidiation du Neurospora crassa. Path. et Microbiol. (Basel) 30, 953–958 (1967).
Davis, B. J.: Disc electrophoresis. Practical aspects. Ann. N.Y. Acad. Sci. 121, 404–416 (1964).
Flavell, R. B., Fincham, J. R. S. Acetate-nonutilising mutants of Neurospora crassa. II. Biochemical deficiencies and the role of certain enzymes. J. Bact 95, 1063–1068 (1968).
Hiatt, A. J.: Preparation and some properties of soluble succinic-dehydrogenase from higher plants. Plant Physiol. 36, 552–557 (1961).
Holzer, H.: Biochemistry of adaptation in yeast. Aspect of yeast metabolism. 155–178 Oxford-Edinburgh Blackwell Sci. Publ. 1968.
Kaplan, N. O.: Neurospora NADPase. Methods in Enzymology, vol. II. pp. 664 to 666. New York: Acad. Press 1955.
Kobr, M. J., Turian, G., Zimmerman, E. J.: Changes in enzymes regulating isotrate breakdown in Neurospora crassa. Arch. Mikrobiol. 52, 169–177 (1965).
— Vanderhaeghe, F., Combépine, G.: Particulate enzymes of the glyoxylate cycle in Neurospora crassa. Biochem. biophys. Res. Commun. 37, 640–645 (1969).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Mansour, T. E.: Phosphofructokinase. II. Heart muscle. Methods in enzymology, vol. IX, pp. 430–434. New York: Acad. Press 1966.
Ojha, M. N., Turian, G.: Thermostimulation of conidiation and succinic oxidative metabolism of Neurospora crassa. Arch. Mikrobiol. 63, 232–241 (1968).
Ornstein, L.: Disc electrophoresis. I. Background and theory Ann. N.Y. Acad. Sci. 121, 321–403 (1964).
Stine, G. J.: Enzyme activities during the asexual cycle of Neurospora crassa. I. Succinic-dehydrogenase. Canad. J. Microbiol. 13, 1203–1216 (1967).
— Clark, A. M.: Synchronous production of conidiophores and conidia of Neurospora crassa. Canad. J. Microbiol. 13, 447–453 (1967).
Strickland, W. N., Shields, M.: Identification of individual enzymes in crude extracts by acrylamide gel electrophoresis. Neurospora Newsl. 12, 15 (1967).
Sussman, A. S.: Dormancy and spore germination. The Fungi, vol. II, pp. 733–764. New York-London: Acad. Press 1966.
Tsao, M. U.: Dehydrogenases of Neurospora crassa. Science 136, 42–43 (1962).
Turian, G.: L'acétate et son double effet d'induction isocitratasique et de différentiation conidienne chez le Neurospora. C. R. Acad. Sci (Paris) 252 1377–1378 (1961).
— Synthetic conidiogenous media for Neurospora crassa Nature (Lond.) 202, 1240 (1964).
— Combépine, G.: Activité comparée de la transaminase alanine-glyoxylate de Neurospora cultivé en présence de saccharose ou d'acétate. Helv. chim. Acta 46, 2453–2457 (1963).
Weiss, B., Turian, G.: A study of conidiation in Neurospora crassa., J. gen. Microbiol. 44, 407–418 (1966).
Westergaard, M., Mitchell, H. K.: Neurospora. V. A. synthetic medium favoring sexual reproduction. Amer.J. Bot. 34, 573–577 (1947).
Zalokar, M.: Enzyme activity and cell differentiation in Neurospora Amer. J. Bot. 46, 555–559 (1959).
— Cochrane, V. W.: Diphosphopyridine nucleotidase in the life cycle of Neurospora crassa. Amer. J. Bot. 43 107–110 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Combépine, G., Turian, G. Activités de quelques enzymes associés à la conidiogenèse du Neurospora crassa . Archiv. Mikrobiol. 72, 36–47 (1970). https://doi.org/10.1007/BF00411013
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00411013