Abstract
The intracellular concentrations of the monovalent inorganic cations K+ and Na+, low molecular weight carbohydrates and quaternary ammonium compounds have been determined for 4 strains of cyanobacteria (Aphanothece halophytica, Coccochloris elabens, Dactylococcopsis salina and Synechocystis DUN52) originally isolated from hypersaline habitats (i.e. habitats with a salinity greater than that of seawater) over a range of external salt concentration (from 50% to 400% seawater). Intracellular cation levels (Na+ and K+) were determined to be within the range 80–320 mmol · dm-3 (cell volume), showing only minor changes in response to salinity. Intracellular carbohydrates were found to comprise a negligible component of the intracellular osmotic potential [at 2–19 mmol · dm-3 (cell volume)], throughout the salinity range. Quaternary ammonium compounds, however, were recorded in osmotically significant quantities [up to 1,640 mmol · dm-3 (cell volume)] in these strains, showing major variation in response to salinity. Thus Synechocystis DUN 52 showed an increase in quaternary ammonium compounds in the oder of 1,200 mmol · dm-3 between 50% and 400% seawater medium, accounting for a significant proportion of the change in external osmotic potential.
Examination of intact cells and cell extracts using 13C and 1H nuclear magnetic resonance (NMR) spectroscopy confirmed the presence of the quaternary ammonium compound glycine betaine as the major osmoticum in the 4 strains; no other compounds were detected during NMR assays. These results suggest a common mechanism of osmotic adjustment, involving quaternary ammonium compounds, in cyanobacteria from hypersaline environments.
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References
Blumwald E, Tel-Or E (1982) Osmoregulation and cell composition in salt-adaptation of Nostoc muscorum. Arch Microbiol 132:168–172
Borowitzka LJ, Brown AD (1984) The salt relations of marine and halophilic species of the unicellular green alga Dunaliella. The role of glycerol as a compatible solute. Arch Microbiol 96:37–52
Borowitzka LJ, Demmerle S, Mackay MA, Norton RS (1980) Carbon-13 nuclear magnetic resonance study of osmoregulation in a blue-green alga. Science 210:650–651
Brock TD (1976) Halophilic-blue-green algae. Arch Microbiol 107:109–111
Brown AD (1976) Microbial water stress. Bacteriol Rev 40:803–846
Brown AD (1978) Compatible solutes and extreme water stress in eukaryotic microorganisms. Adv Microb Physiol 17:181–242
Brown AD (1983) Halophilic prokaryotes. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Encyclopedia of plant physiology, vol 12c. Springer, Berlin Heidelberg New York, pp 137–162
Cohen Y, Krumbein WE, Shilo M (1977) Solar Lake (Sinai). 2. Distribution of photosynthetic microorganisms and primary production. Limnol Oceanogr 22:609–620
Erdmann N (1983) Organic osmoregulatory solutes in blue-green algae. Z Pflanzenphysiol 110:147–155
Galinski EA, Trüper HG (1982) Betaine, a compatible solute in the extremely halophilic phototrophic bacterium Ectothiorhodospira halochloris. FEMS Microbiol Lett 13:357–360
Golubic S (1980) Halophily and halotolerance in cyanophytes. Origins of Life 10:169–183
Greenway H, Munns R, Gibbs J (1982) Effects of accumulation of 3-O-methylglucose on levels of endogenous osmotic solutes in Chlorella emersonii. Plant Cell Environ 5:405–412
Hof T, Frémy P (1933) On Myxophyceae living in strong brines. Rec Trav bot Neerl 30:140–162
Holligan PM, Drew EA (1971) Routine analysis by gas-liquid chromatography of soluble carbohydrates in extracts of plant tissues. II. Quantitative analysis of standard carbohydrates and the separation and estimation of soluble sugars from a variety of plant tissues. New Phytol 70:270–297
Miller DM, Jones JH, Yopp JH, Tindall DR, Schmid WD (1976) Ion metabolism in a halophilic blue-green alga, Aphanothece halophytica. Arch Microbiol 111:145–149
Mohammad FAA, Reed RH, Stewart WDP (1983) The halophilic cyanobacterium Synechocystis DUN52 and its osmotic responses. FEMS Microbiol Lett 16:287–290
Munns R, Greenway H, Kirst GO (1983) Halotolerant eukaryotes. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Encyclopedia of plant physiology, vol 12c. Springer, Berlin Heidelberg New York, pp 59–135
Pillai VK (1955) Observations on the ionic composition of blue-green algae growing in saline lagoons. Proc nat Inst Sci India B: Biol Sci 21:90–102
Pollard A, Wyn Jones RG (1979) Enzyme activites in concentrated solutions of glycinebetaine and other solutes. Planta 144:291–298
Reed RH, Collins JC (1980) The ionic relations of Porphyra purpurea (Roth) C. Ag (Rhodophyta Bangiales). Plant Cell Environ 3:399–407
Reed RH, Stewart WDP (1983) Physiological responses of Rivularia atra to salinity: osmotic adjustment in hyposaline media. New Phytol 95:595–603
Reed RH, Collins JC, Russell G (1980) The effects of salinity upon galactosyl-glycerol content and concentration of the marine red alga Porphyra purpurea (Roth) C. Ag. J exp Bot 31:1539–1554
Reed RH, Rowell P, Stewart WDP (1981) Characterization of the transport of potassium ions in the cyanobacterium Anabaena variabilis Kütz. Eur J Biochem 116:323–330
Reed RH, Richardson DL, Warr SRC, Stewart WDP (1984) Carbohydrate accumulation and osmotic stress in cyanobacteria. J Gen Microbiol 130:1–4
Richardson DL, Reed RH, Stewart WDP (1983) Synechocystis PCC6803: a euryhaline cyanobacterium. FEMS Microbiol Lett 18:99–102
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171–205
Storey R, Wyn Jones RG (1977) Responses of Atriplex spongiosa and Suaeda monoica to salinity. Plant Physiol 63:156–162
Wall JS, Christianson DD, Dimler RJ, Santi FR (1960) Spectrophotometric determination of betaines and other quaternary ammonium compounds as their periodides. Anal Chem 32:870–874
Walsby AE, Van Rijn J, Cohen Y (1983) The biology of a new gasvacuolate cyanobacterium, Dactylococcopsis salina sp nov, in Solar Lake. Proc R Soc Lond 217:417–447
Warr SRC, Reed RH, Stewart WDP (1984) Osmotic adjustment of cyanobacteria: the effects of NaCl, sucrose and glycine betaine on glutamine synthetase activity in a marine and a halotolerant strain. J Gen Microbiol 130 (in press)
Wyn Jones RG, Gorham J (1983) Osmoregulation. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Encyclopedia of plant physiology, vol 12c. Springer, Berlin Heidelberg New York, pp 35–58
Yopp JH, Miller DM, Tindall DR (1978) Regulation of intracellular water potential in the halophilic blue-green alga Aphanothece halophytica (Chroococcales) In: Caplan SR, Ginzburg M (eds) Energetics and structure of halophilic microorganisms. Elsevier, Amsterdam, pp 619–624
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Reed, R.H., Chudek, J.A., Foster, R. et al. Osmotic adjustment in cyanobacteria from hypersaline environments. Arch. Microbiol. 138, 333–337 (1984). https://doi.org/10.1007/BF00410900
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DOI: https://doi.org/10.1007/BF00410900