Summary
The uphill monosaccharide transport in the yeast Rhodotorula gracilis has been investigated under conditions of blocked energy supply:
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1.
The presence of metabolic inhibitors or uncouplers, such as NaN3, 2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, potassium sorbate, limited the monosaccharide uptake exclusively to the cell wall space. Thus, the plasma membrane of the yeast appears to be impermeable to monosaccharides, unless the carrier mediated energy requiring transport system is operating.
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2.
The steady-state kinetics of 14C-d-xylose exchange showed a manifold decrease in the rate constant of efflux, when the energy supply was suspended. The fraction of cellular sugar content comprising this compartment amounted to approximately 75% of the total. The rest was ascribed to the cell wall space and to the extracellular space.
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3.
Anaerobic conditions resulted in the same effects as did the addition of inhibitors.
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4.
Potassium sorbate, an inhibitor shown to uncouple the uphill transport of monosaccharides from cell metabolism in Candida beverwijkii, did not display a similar effect in R. gracilis suspensions.
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Höfer, M. Transport of monosaccharides in Rhodotorula gracilis in the absence of metabolic energy. Arch. Mikrobiol. 80, 50–61 (1971). https://doi.org/10.1007/BF00410579
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DOI: https://doi.org/10.1007/BF00410579