Skip to main content
SpringerLink
Log in

Scanning electron microscopy of frozen-cracked, dry-cracked and enzyme-digested tissue of human malignant choroidal melanomas

  • Published:
Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Aims and scope Submit manuscript

Abstract

By applying cracking and enzyme-digestion methods to tissue of human malignant choroidal melanomas, SEM was used to visualize the interior of the cells, their membranes, nuclei and organelles.

Single cells were depicted to their full extent. Apart from a three-dimensional impression of the structures and their surfaces, the methods also made it much easier to measure cells and cell components than by conventional methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altmann, H.W.: Zellkern. In Hirsch, G.C., Ruska, H., Sitte, P. (ed.): Grundlagen der Cytologie, p. 136. Stuttgart: Fischer, 1973

    Google Scholar 

  • Drzewiecki, K.T.: The surface morphology of the melanoma cell. A scanning electron microscope study on the primary cutaneous melanoma. Scand. J. Plast. Reconstr. Surg. 11, 9–16 (1977)

    Google Scholar 

  • Drzewiecki, K.T., Kjærgaard, J.: Scanning electron microscopy of melanocytes in normal human epidermis. Proc. 9. Congr. Nord. Soc. Cell Biol., Odense University Press 1976, pp. 171–176 (1975)

  • Drzewiecki, K.T., Kjærgaard, J.: Use of scanning electron microscopy for the study of human epidermal melanocytes. Scand. J. Plast. Reconstr. Surg. 11, 3–8 (1977)

    Google Scholar 

  • Hirsch, M., Renard, G., Faure, J.-P., Pouliquen, Y.: Study of the ultrastructure of the rabbit corneal endothelium by the freeze fracture technique: apical and lateral junctions. Exp. Eye Res. 25, 277–288 (1977)

    Google Scholar 

  • Jensen, O.A., Prause, J.U.: Frozen resin cracking, dry cracking, and enzyme digestion methods in SEM as applied to ocular tissues. Albrecht v. Graefes Arch. klin. exp. Ophthal. 212, 207–216 (1980)

    Google Scholar 

  • Radnót, M.: Scanning electron microscopy of melanoblastomas of the choroid. Ophthalmologica (Basel) 173, 352–363 (1976)

    Google Scholar 

  • Tanaka, K.: Frozen resin cracking method and its role in cytology. In Hayat, M.A. (ed.): Principles and techniques of scanning electron microscopy. Biological applications. Vol. 1, pp. 125–134. New York: Van Nostrand Reinhold Co., 1974

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Eye Pathology Institute, University of Copenhagen, Fr. V's Vej 11,5, DK-2100, Copenhagen Ø, Denmark

    J. U. Prause & O. A. Jensen

Authors
  1. J. U. Prause
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. A. Jensen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prause, J.U., Jensen, O.A. Scanning electron microscopy of frozen-cracked, dry-cracked and enzyme-digested tissue of human malignant choroidal melanomas. Albrecht von Graefes Arch. Klin. Ophthalmol. 212, 217–225 (1980). https://doi.org/10.1007/BF00410517

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00410517

Keywords

Search

Navigation