Abstract
An electron microscopic investigation was conducted to study the extracellular materials of the endothelial meshwork in the human outflow apparatus. A quantitative analysis was made to determine the changes which occur with age. Tissue from 20 cases, ages 4 months to 80 years, was examined. A morphometric technique which utilised a grid of 100 random points was used to estimate the percentage area of extracellular components in the endothelial meshwork.
It was found that while the percentage area occupied by amorphous ground substance and fibrillar material decreased with age, the percentage area occupied by the electron-dense component increased. Variation in distribution of these components was noted both between cases of similar age and also within one section. A qualitative description of other changes in the trabecular meshwork is given, and the limitations of the technique are discussed.
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References
Bill, A., Svedbergh, B.: Scanning electron microscopic studies of the trabecular meshwork and the canal of Schlemm — an attempt to localise the main resistance to outflow of aqueous humour in man. Acta. Ophthal. (Kbh). 50, 295–320 (1972)
Fine, B.S.: Observations on the drainage angle in man and rhesus monkey: A concept of the pathogenesis of chronic simple glaucoma. Invest. Ophthal. 3, 609–646 (1964)
Fink, A.I., Felix, M.D., Fletcher, R.C.: The electron microscopy of Schlemm's canal and adjacent structures in glaucomatous patients. Tr. Amer. Ophthal. Soc. 70, 82–102 (1972)
Flocks, M.: The anatomy of the trabecular meshwork as seen in tangential sections. Arch. Ophthal. 56, 708–718 (1956)
Holmberg, Å.S.: Schlemm's canal and the trabecular meshwork. An electron microscopic study of the normal structure in man and monkey (Cercopithecus ethiops). Docum. Ophthalmol. 19, 339–373 (1965)
Rohen, J.W., Lütjen-Drecoll, E.: Age changes of the trabecular meshwork in human and monkey eyes. In “Ageing and Development”, Vol. 1, 1–35, Ed. Bredt and Rohen J.W., Stuttgart: Schattauer 1971
Rohen, J.W., Witmer, R.: Electron microscopic studies on the trabecular meshwork in glaucoma simplex. A. von Graefes Arch. klin. exp. Ophthal. 196, 255–265 (1972)
Segawa, K.: Ultrastructural changes of the trabecular tissues in primary open angle glaucoma. Jap. J. Ophthal. 19, 317–338 (1975)
Speakman, J.S.: Drainage channels in the trabecular wall of Schlemm's canal. Brit. J. Ophthal. 44, 513–523 (1960)
Tripathi, R.: The functional morphology of the outflow systems of ocular and cerebrospinal fluid. Exp. Eye Res. 25, Suppl. 65–116 (1977a)
Tripathi, R.: Pathologic anatomy of the outflow pathway of aqueous humour in chronic simple glaucoma. Exp. Eye Res. 25, Suppl. 403–407 (1977b)
Vegge, T.: The fine structure of trabeculum cribriforme and inner wall of Schlemm's canal in normal human eye. Z. Zellforsch. 77, 267–281 (1967)
Weibel, E.R.: Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol. 20, 235–302 (1969)
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McMenamin, P.G., Lee, W.R. Age related changes in extracellular materials in the inner wall of Schlemm's canal. Albrecht von Graefes Arch. Klin. Ophthalmol. 212, 159–172 (1980). https://doi.org/10.1007/BF00410512
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DOI: https://doi.org/10.1007/BF00410512