Summary
Stock cultures of yeast strains which have acquired the ability to ferment and assimilate various sugars often represent a mixed population in regard to these features.
Usually de-adaptation was not found after cultivation in glucose medium, indicating that the saltations are stable in this medium.
The lyophilized cultures revived in glucose medium have the original characteristics of the strains.
When single-cell colonies of various strains unable to utilize the appropriate sugar were grown in a liquid medium containing either galactose, sucrose or maltose, cells fermenting the sugar offered were found in the cultures. The acquisition of maltose-fermenting ability and the simultaneous acquisition of ability to utilize either galactose and raffinose, sucrose and raffinose, maltose and sucrose, or maltose, sucrose and α-methyl-d-glucoside were observed.
By an identification according to the acquired features the yeasts concerned have to be classified in other species than the original strain.
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References
Avigad, G.: Accumulation of trehalose and sucrose in relation to the metabolism of α-glucosides in yeasts of defined genotype. Biochim. biophys. Acta (Amst.) 40, 124 (1960).
Douglas, H. C., and D. C. Hawthorne: Enzymatic expression and genetic linkage of genes controlling galactose utilization in Saccharomyces. Genetics 49, 837 (1964).
Eddy, A. A.: The role of mutation in the adaptation of a yeast to galactose. Proc. roy. Soc. B 142, 267 (1954).
Halvorson, H. O., S. Winderman, and J. Gorman: Comparison of the α-glucosidases of Saccharomyces produced in response of five non-allelic maltose genes. Biochim. biophys. Acta (Amst.) 67, 42 (1963).
Hartmann, M.: Eine rassenspaltige Torula-Art, welche nur zeitweise Maltose zu vergären vermag (Torula colliculosa n. sp.). Wschr. Brauerei 20, 113 (1903).
Haupt, W., H. Alps u. F. Mugele: Über die Vergärung der Saccharose durch Saccharomyces-Stämme. Arch. Mikrobiol. 45, 276 (1963).
Kudriavzev, V. I.: Experimental change in the physiological properties of yeast. C. R. Acad. Sci. U.R.S.S. 19, 513 (1938).
Laskowski, W.: In F. Reif u. Mitarb.: Die Hefen, Bd. I. Nürnberg 1960.
Lederberg, J., and E. M. Lederberg: Replica plating and indirect selection of bacterial mutants. J. Bact. 63, 399 (1952).
Lindergren, C. C., and G. Lindegren: The genetics of Melezitose fermentation in Saccharomyces. Genetica 26, 430 (1953).
Lodder, J.: Die anaskosporogenen Hefen, I. Hälfte. Verh. Akad. Wet. Amst., Afd. Natuurkunde, sect. 2, 32, I (1934).
—, and N. J. W. Kreger-van Rij: The Yeasts. Amsterdam, North Holland Publishing Co. 1952.
Magni, G. E.: The origin of spontaneous mutations during meiosis. Proc. nat. Acad. Sci. (Wash.) 50, 975 (1963).
Oeser, H., and S. Windisch: Die Bestimmung eines weiteren Maltose-Gens bei Hefen der Gattung Saccharomyces. Naturwissenschaften 51, 122 (1964).
Ogur, M., and A. St. John: A differential and diagnostic plating method for population studies of respiration deficiency in yeast. J. Bact. 72, 500 (1956).
Pappagianis, D., and H. J. Phaff: Delayed fermentation of sucrose by certain species of Saccharomyces. Antonie v. Leeuwenhoek 22, 353 (1956).
Peynaud, E., et S. Domercq: Sur les levures dénomées Saccharomyces steineri et Saccharomyces italicus. Ann. Inst. Pasteur 91, 574 (1956).
Phaff, H. J., and L. do Carmo-Sousa: Four new yeast species isolated from insect frass. Antonie v. Leeuwenhoek 28, 204 (1962).
Roberts, C., A. T. Ganesan, and W. Haupt: Genetics of melibiose fermentation in Saccharomyces italicus var. melibiosi. Heredity 13, 499 (1959).
—, and J. P. van der Walt: Investigations on maltose utilization in Saccharomyces. I. The acquisition of maltose-fermentative ability by Saccharomyces capensis. C. R. Lab. Carlsberg, Sér. Physiol. 32, 19 (1960).
— and J. Wynants: Investigations on maltose utilization in Saccharomyces. II. A genetic study of the maltose-fermenting strain of Sacch. oviformis. C. R. Lab. Carlsberg. Sér. Physiol. 32, 291 (1961).
Rudert, F., and H. O. Halvorson: The effect of gene dosage on the level of α-glucosidase in yeast. Bull. Res. Coun. Israel E 11, 337 (1963).
Santa Maria, J.: New melibiose-utilizing yeasts, isolated from alpechin. Antonie v. Leeuwenhoek 29, 329 (1963).
—: Saccharomyces onubensis, nov. spec., aislada de mosto de uva, y su relacion con Sacch. capensis (synom. Sacch. oviformis). Bol. Inst. Invest. Agronomicas (Madrid) 51, 527 (1964).
Spiegelman, S., R. R. Sussman, and E., Pinska: On the cytoplasmic nature of “longterm adaptation” in yeast. Proc. nat. Acad. Sci. (Wash) 36, 591 (1950).
Walt, J. R. van der: Saccharomyces vafer and S. inconspicuous spp. n. Antonie v. Leeuwenhoek 31, 187 (1965).
—, and I. T. Tscheuschner: Saccharomyces capensis nov. spec. a new yeast from South African grape must. Antonie v. Leeuwenhoek 22, 257 (1956).
Wickerham, L. J.: Taxonomy of yeasts. Tech. Bull. U.S. Dep. Agric. No. 1029 (1951).
—, and K. A. Burton: Carbon assimilation tests for the classification of yeasts. J. Bact. 56, 363 (1948).
Windisch, S.: Über die Ergebnisse weiterer vegetationskundlicher Analysen an Bieren. Mischr. Brauerei 15, 203 (1962).
Winge, O., and C. Roberts: The polymeric genes for maltose fermentation in yeasts, and their mutability. C. R. Lab. Carlsberg 25, 35 (1950).
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Scheda, R., Yarrow, D. The instability of physiological properties used as criteria in the taxonomy of yeasts. Archiv. Mikrobiol. 55, 209–225 (1966). https://doi.org/10.1007/BF00410244
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DOI: https://doi.org/10.1007/BF00410244