Abstract
The nature of the endogenous reserves of Saccharomyces cerevisiae was examined with respect to conditions of growth, specifically extremes of oxygen tension and carbon source. Cells were grown in batch culture at 30 C under aerobic conditions on a galactose or glucose carbon source and under anaerobic conditions on glucose. The greatest effect of growth conditions on the chemical composition of the cells was on their fatty acid and sterol content.
Cells grown under both aerobic and anaerobic conditions mobilised concurrently protein, glycogen, trehalose and fatty acids during a period of 72 hours' starvation under aerobic conditions. The viability of both types of the aerobically grown cells declined to 75% during this period and was not influenced by the initial fatty acid and sterol content of the cells. Cells grown anaerobically showed a more rapid decline in viability which was only 17% after 72 hours' starvation. This loss of viability was not due to a lack of available endogenous reserves but was probably due to an impaired membrane function caused by a deficiency of sterols and unsaturated fatty acids.
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References
Andreasen, A. A. and Stier, T. J. B. 1953. Anaerobic nutrition of Saccharomyces cerevisiae. — J. Cell. Comp. Physiol. 41: 23–36.
Bulder, C. J. E. A. and Reinink, M. 1974. Unsaturated fatty acid composition of wild type and respiratory deficient yeasts after aerobic and anaerobic growth. — Antonie van Leeuwenhoek 40: 445–455.
Brown, C. M. and Johnson, B. 1971. Influence of oxygen tension on the physiology of Saccharomyces cerevisiae in continuous culture. — Antonie van Leeuwenhoek 37: 477–487.
Chester, V. E. 1963. The dissimilation of the carbohydrate reserves in a strain of Saccharomyces cerevisiae. — Biochem. J. 86: 153–160.
Chester, V. E. 1964. Comparative studies on the dissimilation of reserve carbohydrates in four strains of Saccharomyces cerevisiae. — Biochem. J. 92: 318–323.
Criddle, R. S. and Schatz, G. 1969. Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties. — Biochemistry 8: 322–334.
Dawes, E. A. and Ribbons, D. W. 1964. Some aspects of the endogenous metabolism of bacteria. — Bacteriol. Rev. 28: 126–149.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. — Anal. Chem. 28: 350–356.
Gronlund, A. F. and Campbell, J. J. R. 1961. Nitrogenous compounds as substrates for endogenous respiration in microorganisms. — J. Bacteriol. 81: 721–724.
Halvorson, H. 1958. Intracellular protein and nucleic acid turnover in resting yeast cells. —Biochim. Biophys. Acta 27: 255–266.
Hunter, K. and Rose, A. H. 1972. Lipid composition of Saccharomyces cerevisiae as influenced by growth temperature. — Biochim. Biophys. Acta 260: 639–653.
Jollow, D., Kellermann, G. M. and Linnane, A. W. 1968. The biogenesis of mitochondria. III. The lipid composition of aerobically and anaerobically grown Saccharomyces cerevisiae as related to the membrane systems of the cells. — J. Cell. Biol. 37: 221–230.
Katsuki, H. and Block, K. 1967. Studies on the biosynthesis of ergosterol in yeast. Formation of methylated intermediates. — J. Biol. Chem. 242: 222–227.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. — J. Biol. Chem. 193: 265–275.
Moore, P. R. and Baumann, C. A. 1952. Skin sterols. 1. Colorimetric determination of cholesterol and other sterols in skin. — J. Biol. Chem. 195: 615–621.
Panek, A. 1963. Function of trehalose in baker's yeast (Saccharomyces cerevisiae). — Arch. Biochem. Biophys. 100: 422–425.
Polakis, E. S. and Bartley, W. 1965. Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. — Biochem. J. 97: 284–297.
Polakis, E. S., Bartley, W. and Meek, G. A. 1965. Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources. — Biochem. J. 97: 298–302.
Postgate, J. R. 1967. Viability measurements and the survival of microbes under minimum stress, p. 1–23. In A. H. Rose and J. F. Wilkinson, (eds.), Advan. Microb. Physiol., Vol. 1. — Academic Press, London.
Reiner, J. M., Gest, H. and Kamen, M. D. 1949. Effect of substrates on the endogenous metabolism of living yeast. — Arch. Biochem. 20: 175–177.
Strittmatter, C. F. 1957. Adaptive variation in the level of oxidtive activity in Saccharomyces cerevisiae. — J. Gen. Microbiol. 16: 169–183.
Swanson, W. H. and Clifton, C. E. 1948. Growth and assimilation in cultures of Saccharomyces cerevisiae. — J. Bacteriol. 56: 115–124.
Trevelyan, W. E. 1966. Determination of some lipid constituents of baker's yeast. — J. Inst. Brewing 72: 184–192.
Trevelyan, W. E. and Harrison, J. S. 1956. Studies on yeast metabolism. 7. Yeast carbohydrate fractions. Separation from nucleic acid, analysis, and behaviour during anaerobic fermentation. — Biochem. J. 63: 23–33.
Wallace, P. G., Huang, M. and Linnane, A. W. 1968. The biogenesis of mitochondria. II. The influence of medium composition on the cytology of anaerobically grown Saccharomyces cerevisiae. — J. Cell Biol. 37: 207–220.
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Wilson, K., McLeod, B.J. The influence of conditions of growth on the endogenous metabolism of Saccharomyces cerevisiae: effect on protein, carbohydrate, sterol and fatty acid content and on viability. Antonie van Leeuwenhoek 42, 397–410 (1976). https://doi.org/10.1007/BF00410171
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DOI: https://doi.org/10.1007/BF00410171