Summary
Five mutant strains of Hydrogenomonas H 16 which synthesize poly-β-hydroxybutyric acid either slowly or not at all have been isolated following nitrite and NMG treatment of wild type cells. When grown on a nitrogen deficient agar medium, the colonies of PHB-free cells can be recognized by their diminished retention of the dye sudanblack B. Enrichment procedures for such mutants have been devised employing the 32P-phosphate inactivation technique and sucrose gradient centrifugation. The mutants have been characterized with respect to their growth properties, respiratory control and other properties.
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Schlegel, H.G., Lafferty, R. & Krauss, I. The isolation of mutants not accumulating poly-β-hydroxybutyric acid. Archiv. Mikrobiol. 71, 283–294 (1970). https://doi.org/10.1007/BF00410161
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DOI: https://doi.org/10.1007/BF00410161