Skip to main content
Log in

The predominant anaerobe from the spiral intestine of hatchery-raised sturgeon (Acipenser transmontanus), a new Bacteroides species

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The anaerobic and aerobic bacterial flora from the spiral intestine of hatchery-raised sturgeon were enumerated. Among the obligate anaerobes, a new bacteroidelike organism was isolated and found to be the predominant strict anaerobe. The organism is Gram-negative, produces the fermentation products acetate, succinate, propionate and H2. The mol % G+C of DNA from various strains ranged from 33.17–33.65. The optimum sodium concentration for this organism was found to be 137 mM; the sodium level in the sturgeon spiral intestine ranged from 94.5–110.0mM. Based on these and other physiological characteristics, the organism appears to be a new species of the genus Bacteroides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

GI:

Gastrointestinal

VFA:

Volatile fatty acid

SI:

Spiral intestine

GLC:

Gas-liquid chromatography

References

  • Baumann P, Baumann L (1977) Biology of the marine enterobacteria: genera Beneckea and Photobacterium. Ann Rev Microbiol 31:39–61

    Article  Google Scholar 

  • Buchanan RE, Gibbons NE (eds) (1974) Bergey's manual of determinative bacteriology, 8th ed. The Williams & Wilkins Co., Baltimore

    Google Scholar 

  • Caldwell DR, Arcand C (1974) Inorganic and metal-organic growth requirements of the genus Bacteroides. J Bacteriol 120:322–333

    PubMed  Google Scholar 

  • Caldwell DR, Keeney M, Barton JS, Kelley JF (1973) Sodium and other inorganic growth requirements of Bacteroides amylophilus. J Bacteriol 114:782–789

    PubMed  Google Scholar 

  • Doroshov S, Clark WH, Lutes PB, Swallow RL, Beer KE, McGuire AB, Cochran MD (1983) Artificial propagation of the white sturgeon Acipenser transmontanus Richardson. Aquaculture 32:93–104

    Article  Google Scholar 

  • Holdeman LV, Cato EP, Moore WEC (1977) Anaerobe Laboratory Manual, 4th Edition. V.P.I. Anaerobe Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, Virginia

    Google Scholar 

  • Horsley RW (1977) A review of the bacterial flora of teleosts and elasmobranchs, including methods for its analysis. J Fish Biol 10:529–553

    Google Scholar 

  • Hungate RE (1966) The rumen and its microbes. Academic Press Inc, New York

    Google Scholar 

  • Hungate RE (1970) A roll tube method for cultivation of strict anaerobes. In: Norris JR, Ribbons DW (eds) Methods in microbiology, Vol. 3B. Academic Press Inc., New York, pp 117–132

    Google Scholar 

  • Macy JM (1981) Nonpathogenic members of the genus Bacteroides. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The Prokaryotes. A handbook on habitats, isolation, and identification of bacteria. Vol. 2. Springer, Berlin, Heidelberg, New York pp 1450–1463

    Google Scholar 

  • Macy JM, Probst I (1979) The biology of gastrointestinal bacteroides. Ann Rev Microbiol 33:561–594

    Article  Google Scholar 

  • Macy JM, Farrand JR, Montgomery L (1982) Cellulolytic and non-cellulolytic bacteria in rat gastrointestinal tracts. Appl Environ Microbiol 44:1428–1434

    Google Scholar 

  • Montgomery L, Macy JM (1982) Characterization of rat cecum cellulolytic bacteria. Appl Environ Microbiol 44:1435–1443

    PubMed  Google Scholar 

  • Pfennig N, Lippert K-D (1966) Über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch Mikrobiol 55:245–256

    Google Scholar 

  • Sakata T, Sugita H, Mitsuoka T, Kakimoto D, Kadota H (1980) Isolation and distribution of obligate anaerobic bacteria from the intestines of freshwater fish. Bulletin Japanese Soc Scientific Fisheries 46:1249–1255

    Google Scholar 

  • Sakata T, Sugita H, Mitsuoka T, Kakimoto D, Kadota H (1981) Characteristics of obligate anaerobic bacteria in the intestines of freshwater fish. Bulletin Japanese Soc Scientific Fisheries 47:421–427

    Google Scholar 

  • Scott HW, Dehority BA (1965) Vitamin requirements of several cellulolytic rumen bacteria. J Bacteriol 89:1169–1175

    PubMed  Google Scholar 

  • Steen JB (1970) The swim bladder as a hydrostatic organ. In: Hoar WS, Randall DJ (eds). Fish physiology Vol IV. Academic Press, New York, pp 413–443

    Google Scholar 

  • Trust TJ (1975) Facultative anerobic bacteria in the digestive tract of chum salmon (Oncorhynchus keta) maintained in fresh water under defined culture conditions. Appl Microbiol 29:663–668

    PubMed  Google Scholar 

  • Trust TJ, Sparrow RAH (1974) The bacterial flora in the alimentary tract of freshwater salmonid fishes. Can J Microbiol 20:1219–1228

    PubMed  Google Scholar 

  • Trust TJ, Bull LM, Currie BR, Buckley JT (1979) Obligate anaerobic bacteria in the gastrointestinal microflora of the grass carp (Ctenopharyngodon idella), goldfish (Carassius auratus), and rainbow trout (Salmo gairdnerii). J Fish Res Board Can 36:1174–1179

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Callman, J.L., Macy, J.M. The predominant anaerobe from the spiral intestine of hatchery-raised sturgeon (Acipenser transmontanus), a new Bacteroides species. Arch. Microbiol. 140, 57–65 (1984). https://doi.org/10.1007/BF00409772

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00409772

Key words

Navigation