Summary
Bud scars were prepared from the cell walls of Saccharomyces cerevisiae by means of an exo-β-d-(1→3)-glucanase. On acid hydrolysis, these structures were shown to consist of mannose (85%), glucose (4%) and glucosamine (2.7%). In addition the isolated bud scars were incubated either with mercurylabelled Concanavalin A or with Concanavalin A coated colloidal gold. They were then examined by electron microscopy and were found to be heavily marked, indicating the presence of mannan. It was thus shown that mannan is a true component of bud scars, which until now had been thought to consist solely of chitin and glucan.
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Bauer, H., Horisberger, M., Bush, D.A. et al. Mannan as a major component of the bud scars of Saccharomyces cerevisiae . Archiv. Mikrobiol. 85, 202–208 (1972). https://doi.org/10.1007/BF00408845
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DOI: https://doi.org/10.1007/BF00408845