Summary
The effect of lytic enzymes of Micromonospora AS on isolated cell walls and intact or heat killed cells of Candida utilis was investigated. Several substances normally used as stabilizers during protoplast formation were tested for their effect on the lytic action of strepzyme M on intact and dead cells: NaCl and KCl markedly inhibited lysis, sucrose only to 40%. Sorbitol and MgSO4 have no inhibitory effect. MgSO4 was selected for further research as it was found to protect the protoplasts. Phosphate buffer pH 6.8 should not be used at concentrations above 0.01 m. When grown submerged in shaking flasks or in pilot fermentation tanks, in liquid medium containing yeast cells and salts, Micromonospora AS gave the highest yield of lytic enzymes. The strepzyme M preparation is thermolabile.
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Gascon, S., Ochoa, A.G., Novaes, M. et al. Lytic action of strepzymes from micromonospora AS. Archiv. Mikrobiol. 51, 156–167 (1965). https://doi.org/10.1007/BF00408723
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DOI: https://doi.org/10.1007/BF00408723