Zusammenfassung
Die Thylakoide aus Rhodospirillum rubrum und Rhodospirillum molischianum werden nach Homogenisation der Zellen mit Ultraschall durch fraktionierte Zentrifugation isoliert. An diese Membranstrukturen ist das System der Photophosphorylierung gebunden. Die Aktivität dieses Systems in Abhängigkeit vom Redoxpotential des Mediums wird untersucht. Die stärkste Bindung anorganischen Phosphates wird unter Edelgasatmosphäre bei Zusatz von Spuren eines Elektronendonators (0,07 μmol Succinat je Ansatz) beobachtet. Die cyclische Photophosphorylierung wird einerseits durch Sauerstoff und oxydierende Verbindungen wie K3Fe(CN)6 anderseits durch „Überreduktion” mittels reduzierter Redoxverbindungen wie 2,6-Dichlorphenolindophenol oder Phenazinmethosulfat (beide reduziert durch Ascorbat) unter Wasserstoffatmosphäre gehemmt. Die Sauerstoffhemmung kann durch reduziertes Phenazinmethosulfat zu 50% aufgehoben werden. Antimycin A blockiert die lichtabhängige Phosphorylierung; 2,4-Dinitrophenol dagegen hemmt kaum. Die zellfreien Systeme beider Arten zeigen die gleiche Abhängigkeit vom Redoxpotential obwohl R. rubrum wesentlich sauerstofftoleranter ist als R. molischianum und auch durch oxydative Phosphorylierung im Dunkeln ATP bilden kann. Die Befunde sprechen für eine Unabhängigkeit der cyclischen Photophosphorylierung von der Atmungskette und für eine starke Übereinstimmung im Aufbau der Elektronentransportsysteme für die cyclische Photophosphorylierung bei R. rubrum und R. molischianum.
Summary
The isolated thylakoide-structures (chromatophores) of Rhodospirillum molischianum and Rhodospirillum rubrum are investigated with regard to activity of cyclic, light induced phosphorylation. A high activity of the photochemical apparatus needs an optimal external oxidation-reduction potential. Over-oxidation by oxygen or K3[Fe[CN)6] inhibit just as much as over-reduction by hydrogen and N-methyl phenazonium methosulfate or 2,6-dichlorphenol-indophenole both reduced with ascorbate. The highest activity is observed in hydrogen atmosphere without an electron-donator system or in helium with traces (0,07 μmol) of succinate. The inhibitoryeffect of oxygen can partly be compensated by reduced PMS. The photochemical apparatus of R. molischianum and R. rubrum react nearly in the same way on changes of the external oxidation-reduction potential and both systems are strongly inhibited by antimycin A but not by low concentrations of 2,4-dinitrophenole. R. molischianum is a strict anaerobic organism and can grow only in the light and under low oxygen partialpressure. In comparison with it R. rubrum is more oxygen-tolerant. The strain can grow under conditions of aerobic dark metabolism. The present results together with those of other investigations provide strong evidence for the conclusion that the systems of cyclic photophosphorylation in both organisms have the same composition and are relatively independent from the respiratory chain.
Literatur
Arnold, W., and R. K. Clayton: The first step in photosynthesis. Proc. nat. Acad. Sci. (Wash.) 46, 769 (1960).
Arnon, D. I.: Conversion of light into chemical energy in photosynthesis. Nature (Lond.) 184, 10 (1959).
— M. Losada, M. Mozaki, and K. Tagawa: Photoproduction of hydrogen, photofixation of nitrogen and a concept of photosynthesis. Nature (Lond.) 190, 601 (1961).
Baltscheffsky, H., and B. Arwidsson: Evidence for two phosphorylation sites in bacterial cyclic photophosphorylation. Biochim. biophys. Acta (Amst.) 65, 425 (1962).
—, and M. Baltscheffsky: Inhibitor studies on the light-induced phosphorylation. Acta chem. scand. 14, 257 (1960).
Boatman, E. S., and H. C. Douglas: Fine structure of the photosynthetic bacterium Rhodomicrobium vanniellii. J. biophys. biochem. Cytol. 11, 469 (1961).
Bose, S. K., H. Gest, A. W. Frenkel, and K. Cost: Electron transport system in purple bacteria. Nature (Lond.) 195, 1168 (1962).
——: Bacterial photophosphorylation regulation by redox balance. Proc. nat. Acad. Sci. (Wash.) 49, 337 (1963).
Bril, C.: Energy transfer and photooxidative bleaching of bacteriochlorophyll. Biochim. biophys. Acta (Amst.) 66, 50 (1963).
Calvin, M., and G. M. Androes: Primary quantum conversion in photosynthesis. Science 138, 867 (1963).
Chance, B., and M. Nishimura: On the mechanism of chlorophyll-cytochrome interaction. Proc. nat. Acad. Sci. (Wash.) 46, 19 (1960).
Clayton, R. K.: The nature of light-induced absorbancy changes in chromatophores. Photochem. Photobiol. 1, 201 (1962).
—: Primary reactions in bacterial photosynthesis II and III. Photochem. Photobiol. 1, 305, 313 (1962).
Cohen-Bazire, G., and R. Kunisawa: Some observations on the synthesis and function of the photosynthetic apparatus in Rhodospirillum rubrum. Proc. nat. Acad. Sci. (Wash.) 46, 1543 (1960).
—, W. R. Sistrom, and R. Y. Stanier: Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J. cell. comp. Physiol. 49, 25 (1957).
—, and R. Kunisawa: The fine structure of Rhodospirillum rubrum. J. cell. Biol. 16, 401 (1963).
Drews, G.: Untersuchungen zur Struktur der „Chromatophoren” von Rhodospirillum rubrum und R. molischianum. Arch. Mikrobiol. 36, 99 (1960).
—: Physiologische Untersuchungen an den Chromatophoren von Rhodospirillum molischianum. Ber. dtsch. bot. Ges. 75, 338 (1962).
—, u. P. Giesbrecht: Untersuchungen zur Morphogenese der Bakterienchromatophoren und der Bacteriochlorophyllsynthese bei Rhodospirillum rubrum und Rhodopseudomonas spheroides. Zbl. Bakt., I Abt. Orig. 190, 508 (1963).
—, and K. Jäger: Influence of light on the biosynthesis of Bacteriochlorophyll by Rhodopseudomonas spheroides. Nature (Lond.) 199, 1112 (1963).
Frenkel, A. W.: Light-induced reaction of bacterial chromatophores and their relation to photosynthesis. Ann. Rev. Plant. Physiol. 10, 53 (1959).
Geller, D. M.: Oxidative phosphorylation in extracts of Rhodospirillum rubrum. J. biol. Chem. 237, 2947 (1962).
—, and F. Lipmann: Photophosphorylation in extracts of Rhodospirillum rubrum. J. biol. Chem. 235, 2478 (1960).
Giesberger, G.: Some observations on the culture, physiology and morphology of some brown-red Rhodospirillum species. J. Microbiol. Serol. (Ant. van Leeuwenhoek) 13, 135 (1947).
Giesbrecht, P., u. G. Drews: Elektronenmikroskopische Untersuchungen über die Entwicklung der „Chromatophoren” von Rhodospirillum molischianum. Arch. Mikrobiol. 43, 152 (1962).
Goedheer, J. C.: Spectral and redox properties of bacteriochlorophyll in its natural state. Biochim. biophys. Acta (Amst.) 38, 389 (1960).
—: Reversible and irreversible changes of bacteriochlorophyll in chromatophores. J. Bock Carneg. Inst. Wash. 57, 295 (1958).
Horio, T., and M. D. Kamen: Observations on the respiratory system of Rhodospirillum rubrum. Biochem. 1, 1141 (1962).
Jacobi, G.: Die Stöchiometrie der Photosynthese in isolierten Chloroplasten. Z. Naturforsch. 18b, 711 (1963).
Klingenberg, M.: Reversibilität der Energieumwandlungen in der Atmungskette. Angew. Chem. 75, 900 (1963).
Losada, M., A. V. Trebst, S. Ogata, and D. I. Arnon: Equivalence of light and ATP in bacterial photosynthesis. Nature (Lond.) 186, 753 (1960).
Menke, W.: Structure and chemistry of plastids. Ann. Rev. Plant. Physiol. 13, 27 (1962).
Müller, A., B. Rumberg, and H. T. Witt: On the mechanism of photosynthesis. Proc. roy. Soc. B 157, 313 (1963).
Niklowitz, W., u. G. Drews: Zur elektronenmikroskopischen Darstellung der Feinstruktur von Rhodospirillum rubrum. Arch. Mikrobiol. 23, 123 (1955).
Nishimura, M.: Kinetics of photophosphorylation in Rhodospirillum rubrum. Biochim. biophys. Acta (Amst.) 57, 88 (1962).
—: Effects of reagents and temperature on light-induced and dark phases of photophosphorylation. Biochim. biophys. Acta (Amst.) 57, 96 (1962).
Nishimura, M. and B. Chance: Studies on the electron transfer system in photosynthetic bacteria III. Microalgae and Photosynthetic Bacteria (Spec. Issue of Plant and Cell Physiol.) S. 239 (1963).
Ormerod, J. G., and H. Gest: Hydrogen photosynthesis and alternative metabolic pathways in photosynthetic bacteria. Bact. Rev. 26, 51 (1962).
Schachman, H. K., A. B. Pardee, and R. Y. Stanier: Studies in the macromolecular organization of microbial cell. Arch. Biochem. 38, 245 (1952).
Stanier, R. Y.: Photosynthetic mechanism in bacteria and plants. Bact. Rev. 25, 1 (1961).
Sybesma, C., and J. M. Olson: Transfer of chlorophyll exitation energy in green photosynthetic bacteria. Proc. nat. Acad. Sci. (Wash.) 49, 248 (1963).
Tagawa, K., and D. I. Arnon: Ferredoxin as electron carriers in photosynthesis and in the biological production and consumption of hydrogen gas. Nature (Lond.) 195, 537 (1962).
—, H. Y. Tsujimoto, and D. I. Arnon: Role of chloroplast ferredoxin in the energy conversion process of photosynthesis. Proc. nat. Acad. Sci. (Wash.) 49, 567 (1963).
———: Analysis of photosynthetic reactions by the use of monochromatic light. Nature (Lond.) 199, 1247 (1963).
Trebst, A., u. H. Eck: Zur Rolle des Plastochinons bei der Photosynthese. Z. Naturforsch. 18b, 694 (1963).
Vatter, A. E., and R. S. Wolfe: The structure of photosynthetic bacteria. J. Bact. 75, 480 (1958).
Vernon, L. P., and O. K. Ash: Coupled photooxidation and photoreduction reactions and associated phosphorylation by chromatophores of Rhodospirillum rubrum. J. biol. Chem. 235, 2721 (1960).
Vredenberg, W. J., and L. N. M. Duysens: Transfer of energy from bacteriochlorophyll to a reaction centre during bacterial photosynthesis. Nature (Lond.) 197, 355 (1963).
Witt, H. T., A. Müller, and B. Rumberg: Electron-transport system in photosynthesis of green plants. Nature (Lond.) 197, 987 (1963).
Zaugg, W. S.: Coupled photoreduction of ubiquinone and photooxydation of ferrocytochrome c. Proc. nat. Acad. Sci. (Wash.) 50, 100 (1963).
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Drews, G. Untersuchungen zur Photophosphorylierung bei Rhodospirillum molischianum und Rhodospirillum rubrum. Archiv. Mikrobiol. 48, 122–135 (1964). https://doi.org/10.1007/BF00408453
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DOI: https://doi.org/10.1007/BF00408453