Abstract
A newly isolated strain of Oscillatoria tenuis produces geosmin and 2-methylisoborneol (MIB) simultaneously. The intra- and extracellular distribution of both compounds was studied. Geosmin was much less released to the medium compared to MIB. Different amounts of each of the compounds were in different cellular fractions. The geosmin and MIB in these fractions were recoverable by ammonium sulphate precipitation, suggesting binding to protein. Thylakoid and cytoplasmic membranes contained most geosmin while high MIB protein ratios were found in a colourless protein fraction as obtained by gel exclusion chromatography.
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Abbreviations
- MIB:
-
2-methylisoborneol
- SDS:
-
sodium dedecyl sulphate
References
Bentley R, Meganathan R (1981) Geosmin and methylisoborneol biosynthesis in streptomycetes. FEBS Lett 125:220–222
Berglind L, Holtan H, Skulberg OM (1983) Case studies on offlavours in some Norwegian lakes. Wat Sci Technol 15:199–209
Desikachary TV (1959) Cyanophyta. Indian Council Agricult Res, New Delhi, pp 222–223
Esau K (1965) Plant anatomy. John Wiley & Sons, New York
Gerber NN (1968) Geosmin, from microorganisms, is trans-1,10-dimethyl-trans-9-decalol. Tetrahedron Lett 25:2971–2974
Gerber NN (1969) A volatile metabolite of actinomycetes, 2-methylisoborneol. J Antibiotics 22:508–509
Izuguirre G, Hwang CJ, Krasner SW, McGuire MJ (1982) Geosmin and 2-methylisoborneol from cyanobacteria in three water supply systems. Appl Environ Microbiol 43:708–714
Jüttner F (1987) Volatile organic substances. In: Fay, P, Van Baalen C (eds) The Cyanobacteria. Elsevier, Amsterdam New York Oxford, pp 453–469
Jüttner F, Leonhardt J, Möhren S (1983) Environmental factors affecting the formation of mesityloxide, dimethylallylic alcohol and other volatile compounds excreted by Anabaena cylindrica. J Gen Microbiol 129:407–412
Lovell RT (1976) Flavour problems in fish culture. FAO Tech Confer Aqucult, Kyoto, Japan
Medsker LL, Jenkins D, Thomas JF, Koch C (1969) Odorous compounds in natural waters. 2-Exo-hydroxy-2-methylbornane, the major odorous compound produced by several actinomycetes. Environ Sci Technol 3:476–477
Ogawa T, Vernon LP (1971) Increased content of cytochrome 554 and 562 in Anabaena variabilis. Biochim Biophys Acta 226:88–97
Persson PE (1983) Off-flavours in aquatic ecosystems. An introduction. Wat Sci Technol 15:1–11
Rippka R, Waterbury JB, Stanier RY (1981) Provisional generic assignments for cyanobacteria in pure culture. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin Heidelberg New York, pp 247–256
Schacterle GR, Pollack RP (1973) A simplified method for the quantitative assay of small amounts of protein in biological material. Analyt Biochem 51:654–655
Tabachek JL, Yurkowski M (1976) Isolation and identification of blue-green algae producing muddy odor metabolites, geosmin and 2-methylisoborneol, in saline lakes in Manitoba. J Fish Res Board Can 33:25–35
Wu JT, Jüttner F (1988) Effect of environmental factors on geosmin production by Fischerella muscicola. Wat Sci Technol (in press)
Yagi M, Kajino M, Matsuo U, Ashitani K, Kita T, Nakamura T (1983) Odour problems in Lake Biwa. Wat Sci Technol 15:311–321
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Wu, JT., Jüttner, F. Differential partitioning of geosmin and 2-methylisoborneol between cellular constituents in Oscillatoria tenuis . Arch. Microbiol. 150, 580–583 (1988). https://doi.org/10.1007/BF00408253
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DOI: https://doi.org/10.1007/BF00408253