Abstract
The cyanobacterium Anabaena variabilis showed a pH dependent uptake of ethylenediamine. No uptake of ethylenediamine was detected at pH 7.0. At higher pH values (e.g. pH 8.0 and pH 9.0) accumulation did occur and was attributed to diffusion of uncharged ethylenediamine in response to a pH gradient. A biphasic pattern of uptake was observed at these higher pH values. Treatment with l-methionine-d,l-sulphoximine (MSX) to inactivate glutamine synthetase (GS) inhibited the second slower phase of uptake without any significant alteration of the initial uptake. Therefore for sustained uptake, metabolism of ethylenediamine via GS was required. NH +4 did not alter the uptake of ethylenediamine. Ethylenediamine was converted in the second phase of uptake to an analogue of glutamine which could not be detected in uptake experiments at pH 7.0 or in uptake experiments at pH 9.0 following pretreatment of cells with MSX. Ethylenediamine treatment inhibited nitrogenase activity and this inhibition was greatest at high pH values.
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Abbreviations
- EDA:
-
1,2-diaminoethane (ethylenediamine)
- GS:
-
glutamine synthetase
- HEPES:
-
4-(2-hydroxyethyl)-1 piperazine ethanesulphonic acid
- MSX:
-
l-methionine-dl-sulphoximine
- ΔΨ:
-
membrane potential
- Tricine:
-
N-tris(hydroxymethyl) methylglycine
References
Bakker EP, Rottenberg H, Kaplan SR (1976) An estimation of the light-induced electrochemical potential difference of protons across the membrane of Halobacterium halobium. Biochim Biophys Acta 440:557–572
Barnes EM, Zimniak P, Jayakumar A (1983) Role of glutamine synthetase in the uptake and metabolism of methylammonium by Azotobacter vinelandii. J Bacteriol 156:752–757
Dharmawardene MWN, Haystead A, Stewart WDP (1973) Glutamine synthetase of the nitrogen-fixing alga Anabaena cylindrica. Arch Microbiol 90:281–295
Kerby NW, Musgrave SC, Codd GA, Rowell P, Stewart WDP (1983) Photoproduction of ammonia by immobilized cyanobacteria. Proceedings: Biotech '83. Online Publications Ltd, Northwood, UK pp 1029–1036
Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322
Musgrave SC, Kerby NW, Codd GA, Stewart WDP (1982a) Sustained ammonia production by immobilized filaments of the nitrogen-fixing cyanobacterium Anabaena 27893. Biotechnol Lett 4:647–652
Musgrave SC, Kerby NW, Codd GA, Stewart WDP (1982b) Structural modifications of calcium alginate entrapped cyanobacteria modified for ammonia production. Eur J Appl Microbiol Biotechnol 17:133–136
Polukhina LE, Sakhurieva GN, Shestakov SV (1982) Ethylenediamine-resistant Anabaena variabilis mutants with derepressed nitrogen-fixing system. Microbiology 51:90–95
Rai AN, Rowell P, Stewart WDP (1984) Evidence for an ammonium transport system in free-living and symbiotic cyanobacteria. Arch Microbiol 137:241–246
Reed RH, Rowell P, Stewart WDP (1980) Components of the proton electrochemical potential gradient in Anabaena variabilis. Biochem Soc Trans 8:707–708
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Sakhurieva GN, Polukhina LE, Shestakov SV (1982) Glutamine synthetase in Anabaena variabilis mutants with derepressed nitrogenase. Microbiology 51:308–312
Sampaio MJAM, Rowell P, Stewart WDP (1979) Purification and some properties of glutamine synthetase from the nitrogenfixing cyanobacteria Anabaena cylindrica and a Nostoc sp. J Gen Microbiol 111:181–191
Scott ID, Nicholls DG (1980) Energy transduction in intact synaptosomes. Influence of plasma-membrane depolarization on the respiration and membrane potential of internal mitochondria determined in situ. Biochem J 186:21–33
Stacey G, Tabita FR, Van Baalen C (1977) Nitrogen and ammonia assimilation in the cyanobacteria: Purification of glutamine synthetase from Anabaena sp. strain CA. J Bacteriol 132:596–603
Stewart WDP (1980) Some aspects of structure and function in N2-fixing cyanobacteria. Annu Rev Microbiol 34:497–536
Stewart WDP (1985) The functional organization of nitrogen-fixing cyanobacteria. Phil Trans Roy Soc Lond B (in press)
Stewart WDP, Rowell P (1975) Effects of l-methionine-dl-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica. Biochem Biophys Res Commun 65:846–857
Stewart WDP, Fitzgerald GP, Burris RH (1967) In situ studies on N2-fixation using the acetylene reduction technique. Proc Natl Acad Sci USA 58:2071–2078
Thomas J, Wolk CP, Shaffer PW, Austin SM, Galonsky A (1975) The initial organic products of fixation of 13N-labelled nitrogen gas by the blue-green alga Anabaena cylindrica. Biochem Biophys Res Commun 67:501–507
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Kerby, N.W., Rowell, P. & Stewart, W.D.P. Ethylenediamine uptake and metabolism in the cyanobacterium Anabaena variabilis . Arch. Microbiol. 141, 244–248 (1985). https://doi.org/10.1007/BF00408066
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DOI: https://doi.org/10.1007/BF00408066