Abstract
DNA of Oncopeltus fasciatus, an organism with diffuse centromeres, has been characterized by determination of its base composition, buoyant density, thermal stability, and reassociation kinetics; renatured DNA was characterized similarly. We conclude that repeated sequences are primarily short and scattered throughout the genome. This is in contrast to the extensive tandem repeats which are found in DNAs of organisms with discrete centromeres.
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Arrighi, F. E., Saunders, P. P., Saunders, G. F., Hsu, T. C.: Distribution of repetitious DNA in human chromosomes. Experientia (Basel) 27, 964–966 (1971)
Bernardi, G.: Chromatography of nucleic acids on hydroxyapatite. Biochim. biophys. Acta (Amst.) 174, 423–448 (1969)
Blumenfeld, M., Forrest, H. S.: Is Drosophila dAT on the Y chromosome? Proc. nat. Acad. Sci. (Wash.) 68, 3145–3149 (1971)
Brahic, M., Fraser, M. J.: Isolation and properties of a rapidly renaturing fraction of mammalian DNA. Biochim. biophys. Acta (Amst.) 240, 23–26 (1971)
Britten, R. J., Kohne, D. E.: Repeated sequences in DNA. Science 161, 529–540 (1968)
Britten, R. J., Smith, J.: A bovine genome. Carnegie Institute of Washington Year Book 68, 385 (1970)
Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956)
Comings, D. E., Okada, T. A.: Holocentric chromosomes in Oncopeltus: kinetochore plates are present in mitosis but absent in meiosis. Chromosoma (Berl.) 37, 177–192 (1972)
Corneo, G., Ginelli, E., Polli, E.: Renaturation properties and localization in heterochromatin of human satellite DNA's. Biochim. biophys. Acta (Amst.) 247, 528–534 (1971)
Davidson, N., Widholm, J., Nandi, U. S., Jensen, R., Olivera, B. M., Wang, J. C.: Preparation and properties of native crab dAT. Proc. nat. Acad. Sci. (Wash.) 53, 111–118 (1965)
Davis, R. W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology, XXI D, p. 413–428. New York: Academic Press 1971
Dickson, E., Boyd, J. B., Laird, C. D.: Sequence diversity of polytene chromosome DNA from Drosophila hydei. J. molec. Biol. 61, 615–627 (1971)
Flamm, W. G., Walker, P. M. B., McCallum, M.: Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus). J. molec. Biol. 40, 423–443 (1969)
Freifelder, D.: The use of NaClO4 to isolate bacteriophage nucleic acids. In: Methods in enzymology, XII A, p. 550–554. New York: Academic Press 1968
French, C. S., Milner, H. W.: Disintegration of bacteria and small particles by high-pressure extrusion. In: Methods in enzymology, I, p. 64–67. New York: Academic Press 1955
Gall, J. G.: Repetitive DNA in Drosophila. In: Symposium on molecular cytogenetics (B. Hamkalo and J. Papaconstantinou, eds.), Gatlinberg Conference. New York: Plenum Publishing Corp. 1973
Gall, J. G., Cohen, E. H., Polan, M. L.: Repetitive DNA sequences in Drosophila. Chromosoma (Berl.) 33, 319–343 (1971)
Günther, H. L., Prusoff, W. H.: Isolation of thymine dimers. In: Methods in enzymology, XII A, p. 19–30. New York: Academic Press 1967
Harris, S. E., Forrest, H. S.: RNA and DNA synthesis in developing eggs of the milkweed bug, Oncopeltus fasciatus (Dallas). Science 156, 1613–1615 (1967)
Hennig, W., Hennig, I., Stein, H.: Repeated sequences in the DNA of Drosophila and their localization in giant chromosomes. Chromosoma (Berl.) 32, 31–63 (1970)
Hughes-Schrader, S., Ris, H.: The diffuse spindle attachment of Coccids, verified by the mitotic behavior of induced chromosome fragments. J. exp. Zool. 87, 429–456 (1941).
Jones, K. W.: Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature (Lond.) 225, 912–915 (1970)
Jones, K. W., Robertson, F. W.: Localisation of reiterated nucleotide sequences in Drosophila and mouse by in situ hybridisation of complementary DNA. Chromosoma (Berl.) 31, 331–345 (1970)
Kit, S.: Equilibrium sedimentation in density gradients of DNA preparations from animal tissues. J. molec. Biol. 3, 711–716 (1961)
Knittel, M. D., Black, C. H., Sandine, W. E., Fraser, D. K.: Use of normal probability paper in determining thermal melting values of deoxyribonucleic acid. Canad. J. Microbiol. 14, 239–245 (1968)
Laird, C. D.: Chromatid structure: relationship between DNA content and nucleotide sequence diversity. Chromosoma (Berl.) 32, 378–406 (1971)
Laird, C. D., McCarthy, B. J.: Molecular characterization of the Drosophila genome. Genetics 63, 865–882 (1969)
Mandel, M., Marmur, J.: Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In: Methods in enzymology, XIIB, p. 195–206. New York: Academic Press 1968
Mandel, M., Schildkraut, C. S., Marmur, J.: Use of CsCl gradient analysis for determining the guanine plus cytosine content of DNA. In: Methods in enzymology, XIIB, p. 184–195. New York: Academic Press 1968
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3, 208–218 (1961)
Martinson, H. G.: Analysis of nucleic acid hybridization mixtures. J. Cell Biol. 55, 165a (1972)
Moore, D. E., Abuelo, J. G.: Human repetitious DNA, satellite DNA and chromosome interarm fibres. Nature (Lond.) 234, 467–468 (1971)
Musich, P. R.: Studies on the DNA of the milkweed bug, Oncopeltus fasciatus. J. Cell Biol. 51, 202a (1971)
Nandi, U. S., Wang, J. C., Davidson, N.: Separation of deoxyribonucleic acids by Hg(II) binding and Cs2SO4 density-gradient centrifugation. Biochemistry 4, 1687–1696 (1965)
Pardue, M. L., Gall, J. G.: Chromosomal localization of mouse satellite DNA. Science 168, 1356–1358 (1970)
Paul, J., Gilmour, R. S.: Organ-specific restriction of transcription in mammalian chromatin. J. molec. Biol. 84, 305–316 (1968)
Rae, P. M. M.: Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 67, 1018–1025 (1970)
Southern, E. M.: Base sequence and evolution of guinea pig α-satellite DNA. Nature (Lond.) 227, 794–798 (1970)
Studier, F. W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)
Sutton, W. D.: A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochim. biophys. Acta (Amst.) 240, 522–531 (1971)
Szybalski, W.: Use of cesium sulfate for equilibrium density gradient centrifugation. In: Methods in enzymology, XIIB, p. 330–360. New York: Academic Press 1968
Walker, P. M. B.: Origin of satellite DNA. Nature (Lond.) 229, 306–308 (1971)
Wetmur, J. G., Davidson, N.: Kinetics of renaturation of DNA. J. molec. Biol. 31, 349–370 (1968)
Wilson, D. A., Bick, M. D., Thomas, C. A.: Eukaryotic DNA contains inverted repetitions. J. Cell Biol. 55, 283a (1972)
Wilson, E. B.: Studies on chromosomes: VIII. Observations on the maturationphenomena in certain hemiptera and other forms, with considerations on synapsis and reduction. J. exp. Zool. 13, 345–449 (1912)
Wolfe, S. L., John, B.: The organization and ultrastructure of male meiotic chromosomes in Oncopeltus fasciatus. Chromosoma (Berl.) 17, 85–103 (1965)
Wu, J.-R., Hurn, J., Bonner, J.: Size and distribution of the repetitive segments of the Drosophila genome. J. molec. Biol. 64, 211–219 (1972)
Yunis, J. J., Yasmineh, W. G.: Satellite DNA in constitutive heterochromatin of the guinea pig. Science 168, 263–265 (1969)
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Lagowski, J.M., Wong Yu, M.Y., Forrest, H.S. et al. Dispersity of repeat DNA sequences in Oncopeltus fasciatus, an organism with diffuse centromeres. Chromosoma 43, 349–373 (1973). https://doi.org/10.1007/BF00406743
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DOI: https://doi.org/10.1007/BF00406743