Abstract
The inability to synthesize cyclic AMP drastically affects the nutritional metabolism of Neurospora crassa. The adenylyl cyclase-less mutant cr-1 (crisp) did not utilize several carbon sources, including glycerol, mannitol, arabinose, and casaminoacids. However, in glucose or acetate it grew as well as the wild type. The following evidence suggested that these nutritional deficiencies were a direct result of the cr-1 mutation: (i), in crosses to wild type they segregated together with the crisp morphological marker; (ii), cyclic AMP added to the cr-1 mutant growth medium overcame the nutritional deficiencies; (iii), the cyclic AMP effect was specific for the crisp mutant, for it was not observed with the wild type, nor with a spontaneous glycerolutilizing cr-1 strain.
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This work was supported by grants from Fundação de Amparo á Pesquisa do Estado de São Paulo (FAPESP-75/779) and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq-2222.0278/75). We are grateful to Dr. Joel L. Greene for helping in preparing the manuscript
Part of a Thesis submitted by J. A. J. to the Department of Biochemistry of the School of Medicine of Ribeirão Preto, University of São Paulo, Brazil, in partial fulfillment of the requirements for the Doctoral degree
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Terenzi, H.F., Jorge, J.A., Roselino, J.E. et al. Adenylyl cyclase deficient cr-1 (Crisp) mutant of Neurospora crassa: Cyclic AMP-dependent nutritional deficiencies. Arch. Microbiol. 123, 251–258 (1979). https://doi.org/10.1007/BF00406658
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DOI: https://doi.org/10.1007/BF00406658