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Verwertung von Fructose durch Hydrogenomonas H16 (I.)

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Zusammenfassung

Die Hydrogenomonas-Stämme H 1, H 16 und H 20 nutzen als einziges Kohlenhydrat Fructose; chemolithotroph gewachsene Zellen des Stammes H 16 oxydieren diesen Zucker nach einer lag-Phase von 20 min.

Die Fructose wird über den Entner-Doudoroff-Weg umgesetzt; während der Adaptation erhöht sich der Gehalt der Zellen an Phosphoglucose-Isomerase, Glucose-6-phosphat-Dehydrogenase und an den für den Entner-Doudoroff-Weg charakteristischen Enzymen.

Die Aktivität der Ribulosediphosphat-Carboxylase geht bei der Adaptation an Fructose innerhalb von 2 Std um 75% zurück, sinkt dann aber während mehrerer Fructose-Passagen nur langsam ab. Folglich kann selbst mit Fructose gewachsener Hydrogenomonas H 16 Kohlendioxyd über den Calvin-Cyclus fixieren.

Summary

The only carbohydrate utilized by Hydrogenomonas strains H 1, H 16 and H 20 is fructose; chemolithotrophically grown cells of strain H 16 oxidize this sugar following a lag-period of 20 min. Fructose is metabolized via the Entner-Doudoroff-pathway. During the adaptation to fructose, the level of the following enzymes increases in the cells: phosphoglucoseisomerase, glucose-6-phosphate-dehydrogenase and the enzymes characteristic of the Entner-Doudoroff-pathway.

During the change from chemolithotrophic to organotrophic growth, with fructose serving as a substrate, the activity of ribulose-diphosphate carboxylase is reduced by 75% within 2 hrs. However, following repeated growth in a fructose medium, this enzyme activity decreases only very slowly. Consequently fructose-grown Hydrogenomonas H 16 is capable of fixing carbon dioxide via the Calvin cycle.

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Literatur

  • Atkinson, D. E.: The biochemistry of Hydrogenomonas. II. The adaptive oxidation of organic compounds. J. Bact. 69, 310 (1955).

    PubMed  Google Scholar 

  • Bartha, R. v.: Physiologische Untersuchungen über den chemolithotrophen Stoffwechsel neu isolierter Hydrogenomonas-Stämme. Arch. Mikrobiol. 41, 313 (1962).

    PubMed  Google Scholar 

  • Bücher, Th.: Über ein phosphatübertragendes Gärungsferment. Biochim. biophys. Acta (Amst.) 1, 292 (1947).

    Article  Google Scholar 

  • Crouch, D. J., and H. H. Ramsey Oxidation of glucose by Hydrogenomonas facilis. J. Bact. 84, 1340 (1962).

    PubMed  Google Scholar 

  • De Ley, J.: Comparative carbohydrate metabolism and localization of enzymes in Pseudomonas and related micro-organisms. J. appl. Bact. 23, 400 (1960).

    Google Scholar 

  • De Ley, J.: Comparative biochemistry and enzymology in bacterial classification. In: 12. Symp. of Soc. gen. Microbiol. 164 (1962).

  • Doudoroff, M.: The oxidative assimilation of sugars and related substances by Pseudomonas saccharophila, with a contribution to the problem of the direct respiration of di- and polysaccharides. Enzymologia 9, 59 (1940).

    Google Scholar 

  • Eagon, R. G., and A. K. Williams: Enzymatic patterns of adaptation to fructose, glucose and mannose exhibited by Pseudomonas aeruginosa. J. Bact. 79, 90 (1960).

    PubMed  Google Scholar 

  • Entner, N., and M. Doudoroff: Glucose and gluconic acid oxidation of Pseudomonas saccharophila. J. biol. Chem. 196, 853 (1952).

    PubMed  Google Scholar 

  • Gottschalk, G.: Die Biosynthese der Poly-β-hydroxybuttersäure durch Knallgasbakterien. III. Synthese aus Kohlendioxyd. Arch. Mikrobiol. 47, 236 (1964).

    PubMed  Google Scholar 

  • Gunsalus, I. C., B. L. Horecker, and W. A. Wood: Pathways of carbohydrate metabolism in microorganisms. Bact. Rev. 19, 79 (1955).

    PubMed  Google Scholar 

  • Hirsch, P.: CO2-Fixierung durch Knallgasbakterien. II. Chromatographischer Nachweis der frühzeitigen Fixierungsprodukte. Arch. Mikrobiol. 46, 53 (1963).

    PubMed  Google Scholar 

  • —, G. Georgiev u. H. G. Schlegel: CO2-Fixierung durch Knallgasbakterien. III. Autotrophe und organotrophe CO2-Fixierung. Arch. Mikrobiol. 46, 79 (1963).

    PubMed  Google Scholar 

  • Horecker, B. L., J. Hurwitz, and A. Weissbach: The enzymatic synthesis and properties of ribulose-1,5-diphosphate. J. biol. Chem. 218, 785 (1956).

    PubMed  Google Scholar 

  • Hughes, D. E.: A press for disrupting bacteria and other microorganisms. Brit. J. exp. Path. 32, 97 (1951).

    PubMed  Google Scholar 

  • Hurlbert, R. E.: Control of carboxydismutase formation in the Thiorhodaceae. J. gen. Microbiol. 31, 17 (1963).

    Google Scholar 

  • Johnson, E. J., and M. K. Johnson: Alternate pathways of glucose metabolism in Azotobacter agilis. Proc. Soc. exp. Biol. (N.Y.) 108, 728 (1961).

    Google Scholar 

  • Katznelson, H.: Production of pyruvate from 6-phosphogluconate by bacterial plant pathogens and legume bacteria. Nature (Lond.) 175, 551 (1955).

    Google Scholar 

  • —: Metabolism of phytopathogenic bacteria. II. Metabolism of carbohydrates by cell-free extracts. J. Bact. 75, 540 (1958).

    PubMed  Google Scholar 

  • Kistner, A.: On a bacterium oxidizing carbon monoxide. Proc. kon. ned. Akad. Wet. 56, 444 (1953).

    Google Scholar 

  • Kluyver, A. J., and A. Manten: Some observations on the metabolism of bacteria oxidizing molecular hydrogen. Antonie v. Leeuwenhoek 8, 71 (1942).

    Google Scholar 

  • Kovachevich, R., and W. A. Wood: Carbohydrate metabolism by Pseudomonas fluorescens. IV Purification and properties of 2-keto-3-deoxy-6-phosphogluconate-aldolase. J. biol. Chem. 213, 757 (1955).

    PubMed  Google Scholar 

  • La Rivière, J. W. M.: On the microbial metabolism of the tartaric acid isomers. Dissertation. Delft 1958.

  • Linday, M., and P. J. Syrett: The induced synthesis of hydrogenase by Hydrogenomonas facilis. J. gen. Microbiol. 19, 223 (1958).

    PubMed  Google Scholar 

  • Ling, K. H., W. L. Byrne, and H. Lardy: Phosphohexokinase. In: S. P. Colowick and N. O. Kaplan: Methods in enzymology. Vol. I, p. 306. New York: Academic Press 1955.

    Google Scholar 

  • MacGee, J., and M. Doudoroff: A new phosphorylated intermediate in glucose oxidation. J. biol. Chem. 210, 617 (1954).

    PubMed  Google Scholar 

  • Metzner, H.: Papierchromatographische Trennung der Photosynthese-Intermediärprodukte. Naturwissenschaften 49, 183 (1962).

    Google Scholar 

  • Mortlock, R. P.: Gluconate metabolism of Pasteurella pestis. J. Bact. 84, 53 (1962).

    PubMed  Google Scholar 

  • Packer, L., and W. Vishniac: Chemosynthetic fixation of carbon dioxide and charakteristics of hydrogenase in resting cell suspensions of Hydrogenomonas ruhlandii nov. spec. J. Bact. 70, 216 (1955).

    PubMed  Google Scholar 

  • Palleroni, N. J., R. Contopoulou, and M. Doudoroff: Metabolism of carbohydrate by Pseudomonas saccharophila. II. Nature of the kinase reaction involving fructose. J. Bact. 71, 202 (1956).

    PubMed  Google Scholar 

  • Peterkofsky, A., and E. Racker: The reductive pentose phosphate cycle. III. Enzyme activities in cell-free extracts of photosynthetic organisms. Plant. Physiol. 36, 409 (1961).

    Google Scholar 

  • Racker, E., and E. A. R. Schroeder: The reductive pentose phosphate cycle. II. Specific C1-phosphatases for fructose-1,6-diphosphate and sedoheptulose-1,7-diphosphate. Arch. Biochem. 74, 326 (1958).

    PubMed  Google Scholar 

  • Santer, M., and W. Vishniac: zit. nach W. Vishniac and P. A. Trudinger: Carbon dioxide fixation and substrate oxidation in the chemosynthetic sulfur and hydrogen bacteria. Bact. Rev. 26, 168 (1962).

    PubMed  Google Scholar 

  • Schatz, A., and C. Bovell: Growth and hydrogenase activity of a new bacterium Hydrogenomonas facilis. J. Bact. 63, 87 (1952).

    PubMed  Google Scholar 

  • Schlegel, H. G., u. R. M. Lafferty: Radioaktivitätsmessungen in Einzellern auf Membranfiltern. Arch. Mikrobiol. 38, 52 (1961).

    PubMed  Google Scholar 

  • —, G. Gottschalk and R. v. Bartha: Formation and utilization of poly-β-hydroxybutyric acid by Knallgas-Bacteria (Hydrogenomonas). Nature (Lond.) 191, 463 (1961).

    Google Scholar 

  • —, H., Kaltwasser u. G. Gottschalk: Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38, 209 (1961).

    PubMed  Google Scholar 

  • Schmidt, K., S. Liaaen Jensen u. H. G. Schlegel: Die Carotinoide der Thiorhodaceae. I. Okenon als Hauptcarotinoid von Chromatium okenii Perty. Arch. Mikrobiol. 46, 117 (1963).

    PubMed  Google Scholar 

  • Sokatch, J. T., and I. C. Gunsalus: Aldonic acid metabolism. I. Pathway of carbon in an inducible gluconate fermentation by Streptococcus faecalis. J. Bact. 73, 452 (1957).

    PubMed  Google Scholar 

  • Stouthamer, A. H.: Glucose and galactose metabolism in Gluconobacter liquefaciens. Biochim. biophys. Acta (Amst.) 48, 484 (1961).

    Article  Google Scholar 

  • Szymona, M., and M. Doudoroff: Carbohydrate metabolism in Rhodopseudomonas spheroides. J. gen. Microbiol. 22, 167 (1960).

    PubMed  Google Scholar 

  • Wang, C. H., C. M. Gilmour, and V. H. Cheldelin: Comparative study of glucose catabolism in microorganisms. Congr. int. Microbiol. Stockholm, p. 146 (1957).

  • Waravdekar, V. S., and L. D. Saslaw: A method of estimation of 2-deoxyribose. Biochim. biophys. Acta (Amst.) 24, 439 (1957).

    Article  Google Scholar 

  • Weissbach, A., and J. Hurwitz: The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. J. biol. Chem. 234, 705 (1959).

    PubMed  Google Scholar 

  • Wilde, E.: Untersuchungen über Wachstum und Speicherstoffsynthese von Hydrogenomonas. Arch. Mikrobiol. 43, 109 (1962).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Deutschland

    G. Gottschalk, U. Eberhardt & H. G. Schlegel

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  1. G. Gottschalk
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  2. U. Eberhardt
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  3. H. G. Schlegel
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Gottschalk, G., Eberhardt, U. & Schlegel, H.G. Verwertung von Fructose durch Hydrogenomonas H16 (I.). Archiv. Mikrobiol. 48, 95–108 (1964). https://doi.org/10.1007/BF00406600

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