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Drug resistance in the fission yeast Schizosaccharomyces pombe: pleiotropic mutations affecting the oleic acid and sterol composition of cell membranes

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Summary

The whole cell lipid and sterol content of the drug resistant strains cyh1, cyh3 and cyh4 was compared with that of wild type by thin layer and gas liquid chromatography and by UV spectrophotometric analysis. The cyh3 and cyh4 strains had a decreased content of the unsaturated 18:1 fatty acid oleic acid, a decreased content of ergosterol and an increased content of 24,28 dehydroergosterol with respect to wild type. The cyh1 strain, however, only showed a decreased content of ergosterol and an increased content of 24,28 dehydro-ergosterol when compared to wild type.

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Author notes

  1. Paul A. Johnston

    Present address: Department of Pharmacology, School of Medicine, University of North Carolina, 27514, Chapel Hill, NC, USA

Authors and Affiliations

  1. School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK

    Paul A. Johnston & Alan Coddington

Authors
  1. Paul A. Johnston
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  2. Alan Coddington
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Johnston, P.A., Coddington, A. Drug resistance in the fission yeast Schizosaccharomyces pombe: pleiotropic mutations affecting the oleic acid and sterol composition of cell membranes. Curr Genet 8, 37–43 (1984). https://doi.org/10.1007/BF00405430

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  • DOI: https://doi.org/10.1007/BF00405430

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