Summary
Mitochondrial DNA (mtDNA) from Lycopersicon esculentum was purified from cell suspension cultures. The DNA, isolated from mitochondria purified by two successive sucrose density gradients, was uncontaminated with nuclear DNA or DNA from proplastids. The total molecular weights of BamHI, BglI, and BglII fragments indicate a mitochondrial genome size of at least 270 kb. Cross hybridization between tomato mtDNA and cloned spinach plastid genes revealed some homology. In hybridization experiments using cloned mitochondrial rRNA genes and BamHI digested total mtDNA the presence of recombination repeats is demonstrated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atchison BA, Whitfeld PR, Bottomley W (1976) Mol Gen Genet 148:263–269
Barbier HA (1980) Dissertation, Universtiy of Science and Medicine, Grenoble
Belliard G, Vedel F, Pelletier G (1979) Nature (London) 281:401–403
Boeshore ML, Hanson MR, Izhar S (1985) Plant Mol Biol 4:125–132
Bohnert HJ, Gordon KHJ, Crouse CJ (1980) Mol Gen Genet 179:539–545
Bolivar F, Rodriguez R, Greene P, Betlach M, Heynecker H, Boyer H, Crosa J, Falkow S (1977) Gene 2:95–113
Brennicke A, Möller S, Blanz PA (1985) Mol Gen Genet 198:404–410
Chao S, Sederoff RR, Levings CS III (1983) Plant Physiol 71:190–193
Chetrit P, Mathieu D, Muller JP, Vedel F (1984) Curr Genet 8:413–421
DeBonte LR, Matthews BF, Wilson KG (1984) Am J Bot 71:932–940
Falconet D, Lejeune B, Quetier F, Gray MW (1984) EMBO J 3:297–302
Goblet JP, Boutry M, Duc G, Briquet M (1983) Plant Mol Biol 2:305–309
Gray MW, Bonen L, Falconet D, Huh TY, Schnare MN, Spencer DF (1982) In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 483–488
Handa H, Tsunewak K, Kunisada T, Yamagashi H (1984) Mol Gen Genet 194:368–372
Kemble RJ, Gunn RE, Flavell RB (1980) Genetics 95:451–458
Kool AJ, DeHaas JM, Mol JMN, VanMarrewijk GAM (1985) Theor Appl Genet 69:223–233
Leaver CJ, Gray MW (1982) Annu Rev Plant Physiol 33:373–402
Lonsdale DM, Hodge TP, Fauron CMR, Flavell RB (1983a) In: Goldberg RB (ed) Plant molecular biology. Liss, New York, pp 445–456 (UCLA Symposium on Molecular and Cellular Biology, vol 12)
Lonsdale DM, Hodge TP, Howe LJ, Stern DB (1983b) Cell 34:1007–1014
Lonsdale DM, Hodge TP, Fauron CMR (1984) Nucleic Acids Res 12:9249–9258
Maniatis T, Fritsch EF, Sambrock J (1982) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
McNay JW, Chourey PS, Pring DR (1984) Theor Appl Genet 67:433–437
Metzlaff M, Börner T, Hagemann R (1981) Theor Appl Genet 60:37–41
Nagy F, Lazar G, Menczel L, Maliga P (1983) Theor Appl Genet 66:203–207
Palmer JD, Shields CR (1984) Nature (London) 307:437–440
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Nature (London) 301:725–728
Southern EM (1975) J Mol Biol 98:503–518
Spencer DF, Williamson S, Schnare MN, Gray MW, Doolittle WF, Bonen L (1983) Endocytobiol II:871–879
Spencer DF, Schnare MN, Gray MW (1984) Proc Natl Acad Sci USA 81:493–497
Stern DB, Lonsdale DM (1982) Nature (London) 299:698–702
Stern DB, Palmer JD (1984a) Nucleic Acids Res 12:6141–6157
Stern DB, Palmer JD (1984b) Proc Natl Acad Sci USA 81:1946–1950
Stern DB, Dyer TA, Lonsdale DM (1982) Nucleic Acids Res 10:3333–3340
Tewes A, Glund K, Walther R, Reinbothe H (1984) Z Pflanzenphysiol 113:141–150
Ward BL, Anderson RS, Bendich AJ (1981) Cell 25:793–803
Whisson DL, Scott S (1985) Plant Mol Biol 4:267–273
Zurawski G, Perrot B, Bottomley W, Whitfeld PR (1981) Nucleic Acids Res 9:3251–3270
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hause, B., Baldauf, F., Stock, K. et al. Molecular analysis of mitochondrial DNA from tomato cell suspension cultures. Curr Genet 10, 785–790 (1986). https://doi.org/10.1007/BF00405102
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00405102