Summary
To clarify the mechanism underlying the deposition of plasma protein in the psoriatic horny layer, extracts of psoriasis scale treated with fibrinolysin and acid citrate buffer were examined using electrophoresis and immunoelectrophoresis. Each extract showed electrophoretic and immunoelec-trophoretic pattern similar to that of normal human serum, although the albumin-globulin ratio was lower than that of normal human serum. Extract after fibrinolysin treatment showed larger amount of substances than that after acid citrate buffer treatment. In each extract, high concentrations of fibrindegrated products were found; fibrinogen was absent. These findings suggest that in the main mechanism of the deposition of plasma protein non-immunological processes are involved, as revealed by our previous study with an immunohistochemical method, and that some fibrinolytic process occurs in psoriatic lesions.
Zusammenfassung
Um den Mechanismus der Ablagerung des Plasmaproteins in der psoriatischen Hornschicht zu klären, wurden Psoriasisschuppenextrakte mit Fibrinolysin und Zitronensäurepufferlösungen behandelt und mittels Elektrophorese und Immunelektrophorese fraktioniert. Die einzelnen Fraktionen zeigten ähnliche elektrophoretische und immunelektrophoretische Bilder wie Normalserum, jedoch war das Verhältnis von Albumin zu Globulin gegenüber Normalserum kleiner. Der Extrakt nach Behandlung mit Fibrinolysin zeigte eine größere Dichte als der nach Behandlung mit Zitronensäurenpufferlösungen. Die Extrakte enthielten kein Fibrinogen, aber viele Fibrinspaltprodukte. Daraus wird geschlossen, daß der Hauptmechanismus der Ablagerung des Plasmaproteins bei Psoriasis kein immunologischer Vorgang ist, sondern daß in den psoriatischen Läsionen ein fibrinolytischer Prozeß stattfindet.
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This research was supported by the grant No. 367201 from the Ministry of Education of Japan
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Kimura, S., Nishikawa, T. A further study on the mechanism of deposition of plasma protein in psoriasis scale. Arch Dermatol Res 268, 79–84 (1980). https://doi.org/10.1007/BF00403889
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DOI: https://doi.org/10.1007/BF00403889