Summary
A method for evaluation of central nervous system depressant activity utilizing potentiation of barbital-Na hypnosis is described. Predetermined doses of barbital-Na which produce hypnosis in 20% of mice or rats (HD 20) are given intraperitoneally with and without treatment with test compounds. The dose of a compound required to produce hypnosis in 80% of the animals (HD 80) may be a useful index for quantitating CNS depressant activity.
A simple screening method for CNS depressants using the HD 20 of barbital-Na with an arbitrarily chosen dose of the test compound and subjecting the results to a chi-square analysis for significance is described.
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Fujimori, H. Potentiation of barbital hypnosis as an evaluation method for central nervous system depressants. Psychopharmacologia 7, 374–378 (1965). https://doi.org/10.1007/BF00403761
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DOI: https://doi.org/10.1007/BF00403761