Summary
UCLA-SO-M14, a human melanoma cell line, was cultured for ten passages in vitro. The line was converted to an ascitic form, M14-A, by transplanting M14 into CD-1 nude mice and back into tissue culture. The minimum number of M14-A cells that formed ascites in all mice (within 10–21 days) was 5×105, and over 80% of such mice developed macroscopic liver metastases. M14-A inoculated subcutaneously formed tumor at the site of injection, but rarely led to the development of metastases. However, when M14-A was inoculated subcutaneously in young mice (1–14 days old), more than 50% developed lung (but not liver) metastases. The reproducibility of the formation of ascites and metastases was confirmed by testing M14-A at various passages. M14-A may be useful as a model for the metastatic process in human melanoma.
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Supported by Grants CA00543, CA30647, and CA12582, awarded by the National Cancer Institute, DHHS; and by Medical Research Services of the Veterans Administration
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Katano, M., Saxton, R.E., Cochran, A.J. et al. Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice. J Cancer Res Clin Oncol 108, 197–203 (1984). https://doi.org/10.1007/BF00402467
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DOI: https://doi.org/10.1007/BF00402467