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The monoclonal antibody CR11-351 discriminates HLA-A2 variants identified by T cells

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Abstract

Serologic and immunochemical assays have shown that the monoclonal antibody (MoAb) CR11-351 recognizes a determinant expressed by HLA-A2 and A28 alloantigens. The MoAb CR1 1-351 blocks the cytotoxicity of some, but not all, anti-HLA-A2 and anti-HLA-A28 alloantisera tested. These findings suggest that each allospecificity consists of several determinants, only some of which are spatially close to the determinant defined by the MoAb CR11-351. The binding of the MoAb CR11-351 to HLA-A2 lymphoid cells is not effected by their precoating with the HLA-A, B-specific MoAb CR10-214, Q6/64, and 6/31 but is enhanced by at least 20% by the MoAb CR10-131, CR10-402 and by the β 2-m-specific MoAb NAMB-1. The MoAb CR11-351 did not react with one of four HLA-A2 variants which are indistinguishable with conventional anti-HLA-A2 sera, but are not recognized by “normal” HLA-A2-restricted cytotoxic T cells and possess structurally distinct HLA-A2 heavy chains. Therefore the MoAb CR11-351 provides the first evidence of a serologically detectable difference between the four HLA-A2 variants and “normal” HLA-A2 antigens.

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Abbreviations

β 2-m:

β 2-microglobulin

DDIA:

double determinant immune assay

MoAb:

monoclonal antibody

NP40:

Nonidet P40 detergent

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Authors and Affiliations

  1. Departments of Pathology and Surgery, College of Physicians and Surgeons, Columbia University Medical Center, 630 West 168th Street, 10032, New York, New York

    Carlo Russo, Ah-Kau Ng, Michele A. Pellegrino & Soldano Ferrone

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  1. Carlo Russo
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  2. Ah-Kau Ng
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  3. Michele A. Pellegrino
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  4. Soldano Ferrone
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Russo, C., Ng, AK., Pellegrino, M.A. et al. The monoclonal antibody CR11-351 discriminates HLA-A2 variants identified by T cells. Immunogenetics 18, 23–35 (1983). https://doi.org/10.1007/BF00401353

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  • DOI: https://doi.org/10.1007/BF00401353

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