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Effects of drought on photosynthesis, chlorophyll fluorescence and photoinhibition susceptibility in intact willow leaves

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Abstract

Plants from clonal cuttings of Salix sp. were subjected to a drying cycle of 10 d in a controlled environment. Gas exchange and fluorescence emission were measured on attached leaves. The light-saturated photosynthetic CO2 uptake became progressively inhibited with decreased leaf water potential both at high, and especially, at low intercellular CO2 pressure. The maximal quantum yield of CO2 uptake was more resistant. The inhibition of light-saturated CO2 uptake at leaf water potentials around-10 bar, measured at a natural ambient CO2 concentration, was equally attributable to stomatal and non-stomatal factors, but the further inhibition below this water-stress level was caused solely by non-stomatal factors. The kinetics of fluorescence emission was changed at severe water stress; the slow secondary oscillations of the induction curve were attenuated, and this probably indicates perturbations in the carbon reduction cycle. The influence of light level during the drought period was also studied. Provided the leaves were properly light-acclimated, drought at high and low light levels produced essentially the same effects on photosynthesis. However, low-light-acclimated leaves became more susceptible to photoinhibitory treatment under severe water stress, as compared with well-watered conditions.

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Abbreviations

P i :

intercellular partial pressure of CO2

PPFD:

photosynthetic photon flux density

RuBP:

ribulose 1,5-bisphosphate

ψ L :

leaf water potential

References

  • Beadle, C.L., Jarvis, P.G. (1977) The effects of shoot water status on some photosynthetic partial processes in Sitka spruce. Physiol. Plant. 41, 7–13

    Google Scholar 

  • Björkman, O. (1981) Responses to different quantum flux densities. In: Encyclopedia of plant physiology, N.S., vol. 12A: Physiological plant ecology I: Responses to the physical environment, pp. 57–107, Lange, C.L., Nobel, P.S., Osmond, C.B., Ziegler, H., eds. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Björkman, O., Powles, S.B. (1984) Inhibition of photosynthetic reactions under water stress: interaction with light level. Planta 161, 490–504

    Google Scholar 

  • Bradbury, M., Baker, N.R. (1983) Analysis of the induction of chlorophyll fluorescence in leaves and isolated thylakoids: contributions of photochemical and non-photochemical quenching. Proc. R. Soc. London B 220, 251–264

    Google Scholar 

  • Bradford, K.J., Hsiao, T.C. (1982) Physiological responses to moderate water stress. In: Encyclopedia of plant physiology, N.S. vol. 12B: Physiological plant ecology II: Water relations and carbon assimilation, pp. 263–324 Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. eds.. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Cerović, Z.G., Sivak, M.N., Walker, D.A. (1984) Slow secondary fluorescence kinetics associated with the onset of photosynthetic carbon assimilation in intact isolated chloroplasts. Proc. R. Soc. London B 220, 327–338

    Google Scholar 

  • Cornic, G., Miginiac, E. (1983) Nonstomatal inhibition of net CO2 uptake by (±) abscisic acid in Pharbitis nil. Plant Physiol. 73, 529–533

    Google Scholar 

  • Downton, W.J.S. (1983) Osmotic adjustment during water stress protects the photosynthetic apparatus against photoinhibition. Plant Sci. Lett. 30, 137–143

    Google Scholar 

  • Duysens, L.N.M., Sweers, H.E. (1963) Mechanism of two photochemical reactions in algae as studied by means of fluorescence. In: Studies on microalgae and photosynthetic bacteria, pp. 353–372, Japan Soc. Plant Physiol. ed. Univ. of Tokyo Press, Tokyo

    Google Scholar 

  • Edwards, G., Walker, D.A. (1983) C3, C4: Mechanisms, and cellular and environmental regulation of photosynthesis. Blackwell Scientific Publications, Oxford

    Google Scholar 

  • Ehleringer, J.R., Cook, C.S. (1984) Photosynthesis in Encelia farinosa Gray in response to decreasing leaf water potenial. Plant Physiol. 75, 688–693

    Google Scholar 

  • Farquhar, G.D., Sharkey, T.D. (1982) Stomatal conductance and photosynthesis. Annu. Rev. Plant Physiol. 33, 317–345

    Google Scholar 

  • Fry, K.E. (1972) Inhibition of ferricyanide reduction in chloroplasts prepared from water-stressed cotton leaves. Crop Sci. 12, 698–701

    Google Scholar 

  • Govindjee, Downton, W.J.S., Fork, D.C., Armond, P.A. (1981) Chlorophyll a fluorescence transient as an indicator of water potential of leaves. Plant Sci. Lett. 20, 191–194

    Google Scholar 

  • Hällgren, J.-E., Sundbom, E., Strand, M. (1982) Photosynthetic responses to low temperature in Betula pubescens and Betula tortuosa. Physiol. Plant. 54, 275–282

    Google Scholar 

  • Havaux, M., Lannoye, R. (1983) Chlorophyll fluorescence induction: A sensitive indicator of water stress in maize plants. Irrig. Sci. 4, 147–151

    Google Scholar 

  • Huffaker, R.C., Radin, T., Kleinkopf, G.E., Cox, E.L. (1970) Effects of mild water stress on enzymes of nitrate assimilation and of the carboxylative phase of photosynthesis in barley. Crop. Sci. 10, 471–474

    Google Scholar 

  • Johnson, R.R., Frey, N.M., Moss, D.N. (1974) Effect of water stress on photosynthesis and transpiration of flag leaves and spikes of barley and wheat. Crop. Sci. 14, 728–731

    Google Scholar 

  • Jones, H.G. (1973) Moderate-term water stresses and associated changes in some photosynthetic parameters in cotton. New Phytol. 72, 1095–1105

    Google Scholar 

  • Jones, H.G., Fanjul, L. (1983) Effects of water stress on CO2 exchange in apple. In: Proceedings on effects of stress on photosynthesis, pp. 75–84, Marcelle, R., Clijsters, H., Van Poucke, M., eds. Martinus Nijhoff/Dr W. Junk Publishers, The Hague

    Google Scholar 

  • Kaiser, W.M. (1984) Response of photosynthesis and dark-CO2-fixation to light, CO2 and temperature in leaf slices under osmotic stress. J. Exp. Bot. 35, 1145–1155

    Google Scholar 

  • Kaiser, W.M., Heber, U. (1981) Photosynthesis under osmotic stress. Effect of high solute concentrations on the permeability properties of the chloroplast envelope and on the activity of stroma enzymes. Planta 153, 423–429

    Google Scholar 

  • Kaiser, W.M., Kaiser, G., Prachuab, P.K., Wildman, S.G., Heber, U. (1981) Photosynthesis under osmotic stress. Inhibition of photosynthesis of intact chloroplasts, protoplasts, and leaf slices at high osmotic potentials. Planta 153, 416–422

    Google Scholar 

  • Kaiser, W.M., Schwitulla, M., Writh, E. (1983) Reactions in chloroplasts, cytoplasm and mitochondria of leaf slices under osmotic stress. Planta 158, 302–308

    Google Scholar 

  • Keck, R.W., Boyer, J.S. (1974) Chloroplast response to low leaf water potentials III. Differing inhibition of electron transport and photophosphorylation. Plant Physiol. 53, 474–479

    Google Scholar 

  • Krause, G.H., Vernotte, C., Briantais, J.-M. (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim. Biophys. Acta 679, 116–124

    Google Scholar 

  • Ögren, E., Öquist, G. (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. II. Photosynthetic electron transport. Physiol. Plant. 62, 187–192

    Google Scholar 

  • Ögren, E., Öquist, G., Hällgren, J.-E. (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. I. Photosynthesis in vivo. Physiol. Plant. 62, 181–186

    Google Scholar 

  • Öquist, G. (1983) Effects of low temperature on photosynthesis. Plant Cell Environ. 6, 281–300

    Google Scholar 

  • O'Toole, J.C., Crookston, R.K., Treharne, K.J., Ozbun, J.L. (1976) Mesophyll resistance and carboxylase activity. A comparison under water stress conditions. Plant Physiol. 57, 465–468

    Google Scholar 

  • Papageorgiou, G. (1975) Chlorophyll fluorescence: An intrinsic probe of photosynthesis. In: Bioenergetics of photosynthesis, pp. 319–371. Govindjee ed., Academic Press, New York London

    Google Scholar 

  • Plaut, Z. (1971) Inhibition of photosynthetic carbon dioxide fixation in isolated spinach chloroplasts exposed to reduced osmotic potentials. Plant Physiol. 48, 591–595

    Google Scholar 

  • Powles, S.B., Cornic, G., Louason, G. (1984) Photoinhibition of in vivo photosynthesis induced by strong light in the absence of CO2: an appraisal of the hypothesis that photorespiration protects against photoinhibition. Physiol. Vég. 22, 437–446

    Google Scholar 

  • Quick, W.P., Horton, P. (1984) Studies on the induction of chlorophyll fluorescence in barley protoplasts. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient. Proc. R. Soc. London B 220, 371–382

    Google Scholar 

  • Radin, J.W., Ackerson, R.C. (1981) Water relations of cotton plants under nitrogen deficiency. III. Stomatal conductance, photosynthesis, and abscisic acid accumulation during drought. Plant Physiol. 67, 115–119

    Google Scholar 

  • Riegel, C.A. (1974) Comments on “A simple but accurate formula for the saturation vapor pressure over liquid water”. J. Appl. Meteor. 13, 606–607

    Google Scholar 

  • Sankhla, N., Huber, W. (1974) Effect of abscisic acid on the activities of photosynthetic enzymes and 14CO2 fixation products in leaves of Pennisetum typhoides seedlings. Physiol. Plant. 30, 291–294

    Google Scholar 

  • Scholander, P.F., Hammel, H.T., Hemmingsen, E.A., Bradstreet, E.D. (1964) Hydrostatic pressure and osmotic potential in leaves of mangroves and some other plants. Proc. Natl. Acad. Sci. USA 52, 119–125

    Google Scholar 

  • Sharkey, T.D., Badger, M.R. (1982) Effects of water stress on photosynthetic electron transport, photophosphorylation, and metabolite levels of Xanthium strumarium mesophyll cells. Planta 156, 199–206

    Google Scholar 

  • Thorpe, M.R. (1978) Correction of infra-red gas analyser readings for changes in reference tube CO2 concentration. Plant Cell Environ. 1, 59–60

    Google Scholar 

  • Tillberg, E., Dons, C., Haugstad, M., Nilsen, S. (1981) Effect of abscisic acid on CO2 exchange in Lemna gibba. Physiol. Plant. 52, 401–406

    Google Scholar 

  • von Caemmerer, S., Farquhar, G.D. (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153, 376–387

    Google Scholar 

  • von Caemmerer, S., Farquhar, G.D. (1984) Effects of partial defoliation, changes of irradiance during growth, short-term water stress and growth at enhanced p(CO2) on the photosynthetic capacity of leaves of Phaseolus vulgaris L. Planta 160, 320–329

    Google Scholar 

  • Walker, D.A. (1981) Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation. Planta 153, 273–278

    Google Scholar 

  • Walker, D.A., Horton, P., Sivak, M.N., Quick, W.P. (1983) Antiparallel relationship between O2 evolution and slow fluorescence induction kinetics. Photobiochem. Photobiophys. 5, 35–39

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Physiology, University of Umeå, S-901 87, Umeå, Sweden

    E. Ögren & G. Öquist

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  1. E. Ögren
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  2. G. Öquist
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Ögren, E., Öquist, G. Effects of drought on photosynthesis, chlorophyll fluorescence and photoinhibition susceptibility in intact willow leaves. Planta 166, 380–388 (1985). https://doi.org/10.1007/BF00401176

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  • DOI: https://doi.org/10.1007/BF00401176

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