Abstract
Glycoproteins extracted from microsomes of in-vitro-cultured cells of Daucus carota L. cv. US-Harumakigosun were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by peroxidase-conjugated concanavalin A. The appearance of a glycoprotein with Mr 31 000 (GP 31) was correlated with the ability of cells to form somatic embryos. GP 31 appeared in embryogenic cells cultured in 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium, but not in somatic embryos and non-embryogenic cells; it disappeared when the cultures were transferred to auxin-free medium. Another glycoprotein with Mr 32 000 (GP 32) was detected only in non-embryogenic cells, regardless of the presence or absence of 2,4-D. Both glycoproteins, GP 31 and GP 32, were associated with the rough endoplasmic reticulum and were extractable with 0.05% deoxycholate.
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Abbreviations
- Con A:
-
concanavalin A
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- ER:
-
endoplasmic reticulum
- GP 31, GP 32:
-
a glycoprotein with an apparent molecular mass of 31 or 32 kdalton
- kDa:
-
kilodalton
- MS:
-
Murashige and Skoog
- Mr :
-
apparent molecular mass
- SDS-PAGE:
-
sodium dodecylsulfate-polyacrylamide gel electrophoresis
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Dong, J.G., Satoh, S. & Fujii, T. Variation in endoplasmic-reticulum-associated glycoproteins of carrot cells cultured in vitro. Planta 173, 419–423 (1988). https://doi.org/10.1007/BF00401030
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DOI: https://doi.org/10.1007/BF00401030