Summary
The pattern of the increase in cytoplasmic Cai 2+ that glucose produces in beta cells has been reported to be highly variable. Here, we evaluated the influence of the culture duration (1–4 days) and conditions (5–10 mmol/l glucose) on Cai 2+ in normal mouse islets stimulated by glucose. After 1 day of culture in 10 mmol/l glucose, a rise of the glucose concentration from 3 to 15 mmol/l induced a triphasic change of Cai 2+ in the islets. A small initial decrease was followed by a large peak increase and then by regular fast oscillations (∼2.5/min). When the culture was prolonged to 2, 3 and 4 days, the initial decrease became inconsistent and the peak occurred earlier, whereas the oscillations decreased in frequency, increased in duration and eventually disappeared; on day 4 the Cai 2+ rise was sustained. After culture in 5 mmol/l glucose, the pattern of Cai 2+ changes induced by 15 mmol/l glucose was different. The initial decrease was very pronounced, the first peak was delayed and clearly separated from the subsequent oscillations. These were of a mixed type (fast Ca2+ transients on top of slow ones) after 1 day, and of a slow type only after 4 days. These alterations in the Cai 2+ oscillations triggered by glucose could not be ascribed to desynchronization of the signal between different regions of the islets. In conclusion, culturing normal mouse islets in 5 or 10 mmol/l glucose for 1–4 days, markedly alters the characteristics of the changes in Cai 2+ produced by glucose. This pitfall must be borne in mind when studying stimulus-secretion coupling in beta cells from normal or diabetic animals, or from human islets.
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Abbreviations
- Cai 2+ :
-
concentration of cytoplasmic calcium
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Gilon, P., Jonas, J.C. & Henquin, J.C. Culture duration and conditions affect the oscillations of cytoplasmic calcium concentration induced by glucose in mouse pancreatic islets. Diabetologia 37, 1007–1014 (1994). https://doi.org/10.1007/BF00400464
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DOI: https://doi.org/10.1007/BF00400464