Abstract
Light and antimycin markedly affected growth and carotenoid synthesis by Phaffia rhodozyma. Exposure of the yeast to high light intensities on agar plates resulted in growth inhibition and decreased carotenoid synthesis. The carotenoid compositions of the yeast were also notably changed by light. β-zeacarotene increased, whereas β-carotene and xanthophylls decreased including astaxanthin, phoenicoxanthin, and 3-hydroxy-3′, 4′-didehydro-β,Ψ-caroten-4-one (HDCO). In liquid medium, growth of the wild-type strain (UCD-FST-67-385) was inhibited by antimycin, but this inhibition was relieved by exposure to light. Light also stimulated carotenoid synthesis about twofold in these antimycin-treated cells. Light may have rescued growth by induction of an alternative oxidase system which facilitated electron disposal when the main respiratory chain was inhibited by antimycin. Isolation and characterization of the oxidase enzymes should be useful in strain development for increased carotenoid production.
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Abbreviations
- DCIP:
-
2,6-dichlorophenol-indophenol
- HDCO:
-
3-hydroxy-3′, 4′-didehydro-β,Ψ-caroten-4-one, PG-n-propyl gallate
- SHAM:
-
salicylhydroxamic acid
- TTFA:
-
thenoyltrifluoroacetone
References
Alexandre A & Lehninger A (1984) Bypasses of the antimycin. A block of mitochondrial electron transport in relation to ubisemiquinone function. Biochim. Biophys. Acta. 767: 120–129
An G-H, Schuman DB & Johnson EA (1989) Isolation of mutants of Phaffia rhodozyma with increased quantities of astaxanthin. Appl. Environ. Microbiol. 55: 116–124
Andrewes AG, Phaff HJ & Starr MP (1976) Carotenoids of Phaffia rhodozyma, a red pigmented fermenting yeast. Phytochemistry 15: 1003–1007
Batra PP (1967) Mechanism of photoinduced carotenoid synthesis: Induction of carotenoid synthesis by antimycin A in the absence of light in Mycobacterium marinum. J. Biol. Chem. 242: 5630–5635
Batra PP, Gleason Jr RM & Jenkins J (1969) Mechanism of photo-induced and antimycin A-induced carotenoid synthesis in Mycobacterium marinum. Requirements for carotenogenesis and further evidence for protein synthesis following induction. Biochim. Biophys. Acta 177: 124–135
Dogbo O, Laferriere A, D'Harlingue A & Camara B (1988) Carotenoid biosynthesis: Isolation and characterization of a bifunctional enzyme catalyzing the synthesis of phytoene. Proc. Natl. Acad. Sci. USA 85: 7054–7058
Edwards DL & Kwiecienski F (1973) Altered mitochondrial respiration in a chromosomal mutant of Neurospora crassa. J. Bacteriol. 116: 610–618
Edwards DL & Rosenberg E (1976) Regulation of cyanide insensitive respiration in Neurospora. Eur. J. Biochem. 62: 217–221
Henry MF & Nyns EJ (1975) Cyanide-insensitive respiration. An alternative mitochondrial pathway. Sub-Cell. Biochem. 4: 1–65
Johnson EA & Lewis MJ (1979) Astaxanthin formation by the yeast Phaffia rhodozyma. J. Gen. Microbiol. 115: 173–183
Johnson EA, Conklin DE & Lewis MJ (1977) The yeast Phaffia rhodozyma as a dietary pigment source for salmonids and crustaceans. J. Fish. Res. Board Can. 34: 2417–1782
Johnson EA, Villa TG & Lewis MJ (1980) Phaffia rhodozyma as an astaxanthin source in salmonid diets. Aquaculture. 20: 123–134
Krinsky NI (1971) Function. In: Isler O (Ed) Carotenoids (pp 669–717) Birkhauser Verlag Basel und Stuttgart
Matsunaka S, Morita S & Conti SF (1966) Respiratory System of Rhodotorula glutinis I. Inhibitor tolerance and cytochrome components. Plant Physiol. 41: 1364–1369
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428
Miller MW, Yoneyama M & Soneda M (1976) Phaffia, a new yeast in the Deuteromycotina (Blastomycetes), Int. J. Syst. Bacteriol. 26: 286–291
Moore MM, Breedveld MW & Autor AP (1989) The role of carotenoids in preventing oxidative damage in the pigmented yeast, Rhodotorula mucilaginosa. Arch. Biochem. Biophys. 270: 419–431
Phaff HJ, Miller MW, Yoneyama M & Soneda M (1972) A comparative study of the yeast florae associated with trees on the Japanese Islands and on the West Coast of North America. In: Terui G (Ed) Proceedings of the 4th IFS: Fermentation technology today (pp. 759–774) Kyoto, Society of Fermentation Technology, Osaka
Ruddat M & Garber ED (1983) Biochemistry, physiology, and genetics of carotenogenesis in fungi. In: Bennett JW & Ciegler A (Ed) Secondary Metabolism and Differentiation in Fungi. Marcel Dekker, New York.
Schonbaum GR, Bonner WD, Storey BT & Bahr JT (1971) Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 47: 124–128
Shiraishi A & Fujii H (1986) Alternative cyanide-and antimycin A-insensitive respiratory system in Sporobolomyces red yeasts. Agric. Biol. Chem. 50: 447–452
Siedow JN & Girvin ME (1980) Alternative respiratory pathway. Its role in seed respiration and its inhibition by propyl gallate. Plant Physiol. 65: 669–674
Stewart PR (1975) Analytical methods for yeasts. Methods in Cell Biology 12: 111–147
Will III OH, Jankowski P, Jorve K, Kovacs A, Rossing W, Schneider P & Newland NA (1987) A comparison of photokilling among carotene and cytochrome c accumulating strains of the smut fungus Ustilago violacea at specific wavelengths from 400 to 650 nm. Phochem. Photobiol. 45: 609–615.
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An, GH., Johnson, E.A. Influence of light on growth and pigmentation of the yeast Phaffia rhodozyma . Antonie van Leeuwenhoek 57, 191–203 (1990). https://doi.org/10.1007/BF00400151
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DOI: https://doi.org/10.1007/BF00400151