Summary
We did this work to see if there is a correlation between lignin synthesis and the activity of the pentose phosphate pathway. Excision of the third internode of the stem of Coleus blumei Benth. followed by incubation on sucrose and indoleacetic acid led to extensive formation of tracheids. During this lignification we determined the activities of glucose-6-phosphate dehydrogenase and fructose-1,6-diphosphate aldolase, and the extent to which [1-14C]-,[3,4-14C]-, and [6-14C]glucose labelled CO2 and the major cellular components. The results indicate that the pentose phosphate pathway was active during lignification, and that the activity of this pathway relative to glycolysis increased at the onset of lignification. Explants of storage tissue of Helianthus tuberosus L. were cultured under conditions which caused extensive lignification. 14CO2 production from [1-14C]-, [3,4-14C]-, and [6-14C]glucose indicated activity of the pentose phosphate pathway during tracheid formation. We suggest that lignification is accompanied by appreciable activity of the pentose phosphate pathway and that this could provide the reducing power for lignin synthesis.
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Abbreviations
- NADP:
-
nicotinamide-adenine dinucleotide phosphate
- IAA:
-
indoleacetic acid
References
ap Rees, T.: Evidence for the widespread occurrence of induced respiration in slices of plant tissues. Aust. J. biol. Sci. 19, 981–990 (1966)
ap Rees, T.: Pathways of carbohydrate breakdown in higher plants. In: MTP international review of science. Biochemistry 11. Plant Biochemistry, pp. 89–127. Ed.: D.H. Northcote. London: Butterworths 1974
ap Rees, T., Beevers, H.: Pathways of glucose dissimilation in carrot slices. Plant Physiol. 35, 830–838 (1960)
ap Rees, T., Fuller, W.A., Wright, B.W.: Pathways of carbohydrate oxidation during thermogenesis by the spadix of Arum maculatum. Biochim. biophys. Acta (Amst.) 437, 22–35 (1976)
Burrell, M.M., ap Rees, T.: Carbohydrate metabolism of rice leaves infected by Piricularia oryzae. Physiol. Plant Path. 4, 489–496 (1974)
Dalessandro, G.: Interaction of auxin, cytokinin and gibberellin on cell division and xylem differentiation in cultured explants of Jerusalem artichoke. Plant. Cell Physiol. 14, 1167–1176 (1973)
Dalessandro, G., Roberts, L.W.: Induction of xylogenesis in pith parenchyma explants of Lactuca. Amer. J. Bot. 58, 378–385 (1971)
Dick, P.S., ap Rees, T.: The pathway of sugar transport in roots of Pisum sativum. J. exp. Bot. 26, 305–314 (1975)
Fowler, M.W., ap Rees, T.: Carbohydrate oxidation during differentiation in roots of Pisum sativum. Biochim. biophys. Acta (Amst.) 201, 33–44 (1970)
Glock, G.E., McLean, P.: Further studies on the properties and assay of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem. J. 55, 400–408 (1953)
Higuchi, T., Shimada, M.: Changes in activity of shikimate: NADP oxidoreductase in relation to lignification of bamboo. Plant. Cell Physiol. 8, 61–69 (1967)
Higuchi, T., Shimada, M.: Changes in activity of D-glucose-6-phosphate: NADP and 6-phospho-D-gluconate: NADP oxidoreductases in relation to lignification of bamboo. Plant. Cell Physiol. 8, 71–78 (1967)
Layne, E.A.L.: Protein estimation with the Folin Ciocalteu reagent. Eds.: S.P. Colowick and N.O. Kaplan. Methods in enzymology 3, 448–450 (1957)
Pollock, C.J., ap Rees, T.: Effect of cold on glucose metabolism by callus and tubers of Solanum tuberosum. Phytochemistry 14, 1903–1906 (1975)
Roberts, L.W.: The initiation of xylem differentiation. Bot. Rev. 35, 201–250 (1969)
Rubery, P.H., Fosket, D.E.: Changes in phenylalanine ammonialyase activity during xylem differentiation in Coleus and soybean. Planta (Berl.) 87, 54–62 (1969)
Stafford, H.A.: The metabolism of aromatic compounds. Ann. Rev. Plant Physiol. 25, 459–486 (1974)
Ting, I.P., Dugger, W.M.: CO2 metabolism in corn roots. I. Kinetics of carboxylation and decarboxylation. Plant Physiol. 42, 712–718 (1967)
Wong, W.J.L., ap Rees, T.: Carbohydrate oxidation in stele and cortex isolated from roots of Pisum sativum. Biochim. biophys. Acta (Amst.) 252, 296–304 (1971)
Wu, R., Racker, E.: Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of Ascites tumor cells. J. biol. Chem. 234, 1029–1035 (1959)
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Pryke, J.A., ap Rees, T. Activity of the pentose phosphate pathway during lignification. Planta 132, 279–284 (1976). https://doi.org/10.1007/BF00399727
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DOI: https://doi.org/10.1007/BF00399727