Skip to main content
Log in

A colour reaction for the differentiation of ascomycetous and hemibasidiomycetous yeasts

  • Taxonomy
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Seventy yeast strains, representative of twenty-six ascogenous genera, four saprobic hemibasidiomycetous genera and thirteen genera of the Cryptococcales were tested for their reaction with the stabilized aromatic diazonium compound, Diazonium Blue B salt. An aqueous, buffered solution of this compound gave a characteristic red colouration with the colonies of the hemibasidiomycetous species and those Cryptococcales characterized by the hemibasidiomycetous cell-wall type. The characteristic colour reaction was not observed with colonies of either the ascomycetous yeasts or those Cryptococcales characterized by the ascomycetous cell-wall type.

The possible taxonomic use of the colour reaction with Diazonium Blue B salt as an affinitive characteristic is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bartnicki-Garcia, S. 1968. Cell wall chemistry, morphogenesis and taxonomy of fungi. —Ann. Rev. Microbiol. 22: 87–108.

    Google Scholar 

  • Bartnicki-Garcia, S. 1970. Cell wall composition and biochemical markers in fungal phylogeny, p. 81–103. In B. Harborne, (ed.), Phytochemical phylogeny. — Academic Press, New York.

    Google Scholar 

  • Bartnicki-Garcia, S. and McMurrough, I. 1971. Biochemistry of morphogenesis in yeasts, p. 441–491. In A. H. Rose and J. S. Harrison, (eds.), The Yeasts, Vol. 2. — Academic Press, London and New York.

    Google Scholar 

  • Bastide, J.-M., Trave, P. and Bastide, M. 1974. New notions about classification of Fungi imperfecti yeasts, p. 247–248. In H. Klaushofer and U. B. Sleytr, (eds.), Proc. IV. Intern. Symposium on Yeasts (Vienna: 8–12 July, 1974), Part I. — Hochschülerschaft an der Hochschule für Bodenkultur, Wien.

    Google Scholar 

  • Cazin, J., Kozel, T. R., Lupan, D. M. and Burt, W. R. 1969. Extracellular deoxyribonuclease production by yeasts. — J. Bacteriol. 100: 760–762.

    Google Scholar 

  • Donk, M. A. 1973. The Heterobasidiomycetes: a reconnaissance. IV. — Kon. Ned. Akad. Wetensch. Proc. (Ser. C) 76: 109–125.

    Google Scholar 

  • Hopsu-Havu, V. K., Laiho, S. M. and Lundell, E. 1967. A colour test for yeasts based on staining of colonies with aromatic diazonium salts. — Mykosen 10: 23–26.

    Google Scholar 

  • Hütter, R. and De Moss, J. A. 1967. Organization of the tryptophan pathway: a phylogenetic study of fungi. — J. Bacteriol. 94: 1896–1907.

    Google Scholar 

  • Kreger-van Rij, N. J. W. and Veenhuis, M. 1971. A comparative study of the cell wall structure of basidiomycetous and related yeasts. — J. Gen. Microbiol. 68: 87–95.

    Google Scholar 

  • Lillie, R. D. 1969. H. J. Conn's biological stains 8th Ed. — Wilkins and Wilkins Co., Baltimore.

    Google Scholar 

  • Lodder, J. 1970. General classification of the yeasts, p. 1–33. In J.Lodder, (ed.), The Yeasts, 2nd. Ed. — North Holland Publ. Co., Amsterdam.

    Google Scholar 

  • McCully, E. K. and Robinow, C. F. 1972a. Mitosis in heterobasidiomycetous yeasts. I. Leucosporidium scottii (Candida scottii). — J. Cell Sci. 10: 857–881.

    Google Scholar 

  • McCully, E. K. and Robinow, C. F. 1972b. Mitosis in heterobasidiomycetous yeasts. II. Rhodosporidium sp. (Rhodotorula glutinis) and Aessosporon salmonicolor (Sporobolomyces salmonicolor). — J. Cell Sci. 11: 1–31.

    Google Scholar 

  • Phaff, H. J. 1971. Structure and biosynthesis of the yeast cell envelope, p. 135–210. In A. H. Rose and J. S. Harrison, (eds.), The Yeasts, Vol. 2. Academic Press, London and New York.

    Google Scholar 

  • Phaff, H. J. and Spencer, J. F. T. 1969. Improved parameters in the separation of species in the genera Rhodotorula and Cryptococcus, p. 59–65. In Yeasts, Proc. II. Symposium on Yeasts (Bratislava 16–21 July 1966). — Vydavatel'stvo Slovenskej Akadémie Vied Bratislava.

  • Sonck, C. E., Hopsu-Havu, V. K. and Lundell, E. 1968. Diazonium salt (Brentamine Blue — ICI) as an aid in yeast diagnostic, p. 55. In Proc. 18th Meeting Scand. Derm. Ass., Turku. Aug. 22–24, 1968.

  • Sonck, C. E. and Tunnela, E. 1969. Staining with Diazonium-Blue B as an aid in the yeast diagnostic, p. E27–E28. In Yeast Symposium 1969, Proc. 3rd Int. Symp. on Yeast, Delft-The Hague, 2–6 June 1969—Antonie van Leeuwenhoek 35: Supplement.

  • Stahl, E. 1969. Thin-layer chromatography, 2nd Ed., p. 750–751. — Springer Verlag Berlin, Heidelberg and New York.

    Google Scholar 

  • van der Walt, J. P. and Johannsen, E. 1974a. The dangeardien and its significance in the taxonomy of the ascomycetous yeasts. — Antonie van Leeuwenhoek 40: 185–192.

    Google Scholar 

  • van der Walt, J. P. and Johannsen, E. 1974b. Cell-wall structure, mitosis and urease activity in Torulopsis species of high GC content. — Antonie van Leeuwenhoek 40: 417–426.

    Google Scholar 

  • van der Walt, J. P. and Johannsen, E. 1975a. The genus Torulaspora Lindner. — CSIR Research Report No. 325, p. 1–23, Pretoria.

  • van der Walt, J. P. and Johannsen, E. 1975b. Trichosporon terrestre sp. nov. — Antonie van Leeuwenhoek 41: 361–365.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

van der Walt, J.P., Hopsu-Havu, V.K. A colour reaction for the differentiation of ascomycetous and hemibasidiomycetous yeasts. Antonie van Leeuwenhoek 42, 157–163 (1976). https://doi.org/10.1007/BF00399460

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00399460

Keywords

Navigation