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Post-translational modifications of lantibiotics

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Abstract

Several newly reported post-translational modification reactions are involved in lantibiotic biosynthesis. A short overview of the present knowledge on the post-translational modifications and on the enzymes involved in lantibiotic biosynthesis is given. The oxidative decarboxylation of the epidermin precursor peptide EpiA is described in detail. The FMN-containing oxidoreductase EpiD is involved in the formation of the C-terminal S-[(Z)-2-aminovinyl]-D-cysteine residue of epidermin: under reducing conditions the side chain of the C-terminal cysteine residue of EpiA is converted to an enethiol. EpiD has no absolute substrate specificity and can be used for modification of peptides having the C-terminal consensus motif [V/I/L/(M)/F/Y/W]-[A/S/V/T/C/(I/L)]-C.

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Abbreviations

Dha:

2,3-didehydroalanine

Dhb:

(Z)-2,3-didehydrobutyrine

ES-MS:

Electrospray Mass Spectrometry

FAD:

Flavin Adenine Dinucleotide

FMN:

Flavin Mononucleotide

MBP:

Maltose-Binding Protein

TFA:

TrifluoroAcetic Acid

TLC:

Thin-Layer Chromatography

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  1. Mikrobielle Genetik, Universität Tübingen, Waldhäuserstr. 70/8, D-72076, Tübingen, Germany

    Thomas Kupke & Friedrich Götz

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  1. Thomas Kupke
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  2. Friedrich Götz
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Kupke, T., Götz, F. Post-translational modifications of lantibiotics. Antonie van Leeuwenhoek 69, 139–150 (1996). https://doi.org/10.1007/BF00399419

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