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Lipid composition of envelopes, prolamellar bodies and other plastid membranes in etiolated, green and greening wheat leaves

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Summary

Comparative studies of lipid composition were made on prolamellar bodies, envelopes and other plastid membranes separately extracted from etiolated, green or greening (intermittent or continuous light) wheat (Triticum sativum L.) leaves. The different membrane fractions were examined by electron microscopy.

The major lipid was digalactosyldiglyceride in the envelopes and prolamellar bodies and monogalactosyldiglyceride in stroma lamellae and grana. Phosphatidylcholine represented 60% of total phospholipids in the envelopes, 30% in prolamellar bodies and 14% in grana. All types of envelopes had the same lipid proportions.

For all lipids the lowest fatty acid unsaturation was always found in the envelope membranes. The relative amount of {ie193-1} acid in the phosphatidylglycerol of envelopes increased from 4% (etioplasts) to an average of 15% (etiochloroplasts and chloroplasts).

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Abbreviations

DGDG:

digalactosyldiglyceride

MGDG:

monogalactosyldiglyceride

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

phosphatidylglycerol

PI:

phosphatidylinositol

PS:

phosphatidylserine

SL:

sulfolipid

References

  • Allen, C.F., Good, P., Trosper, T., Park, R.B.: Chlorophyll, Glycerolipid and Protein ratios in Spinach chloroplast grana and stroma lamellae. Biochem. biophys. Res. Commun. 48, 907–913 (1972)

    Google Scholar 

  • Bahl, J., Lechevallier, D., Monéger, R.: Etude comparée de l'évolution à l'obscurité et à la lumière des lipides plastidiaux de feuilles étiolées de Blé. Physiol. vég. 12, 229–249 (1974)

    Google Scholar 

  • Bahl, J., Phung-Nhu-Hung, S., Lechevallier, D., Monéger, R.: Effects de la lumière intermittente suivie ou non d'éclairement continu sur les lipides plastidiaux de feuilles étiolées de Blé. Comportement particulier du phosphatidylglycérol. Physiol. vég. 13, 115–124 (1975)

    Google Scholar 

  • Bahl, J., Monéger, R.: Sur les lipides des corps prolamellaires isolés de feuilles de Blé étiolées et verdissantes. C. R. Acad. Sci. (Paris) 282, 177–180 (1976)

    Google Scholar 

  • Cobb, A.H., Wellburn, A.R.: Changes in plastid envelope polypeptides during chloroplast development. Planta (Berl.) 121, 273–283 (1974)

    Google Scholar 

  • Coic, Y.: Comparaison de l'influence de la nutrition nitrique et ammoniacale combinée ou non avec une déficience en acide phosphorique sur l'absorption et la métabolisme des anions, cations et plus particulièrement des acides organiques chez le Maïs. Comparaison du Maïs et de la Tomate quant à l'essai de la nature de l'alimentation azotée. Ann. Physiol. vég. 3, 141–163 (1961)

    Google Scholar 

  • Douce, R., Holtz, R.B., Benson, A.A.: Isolation and properties of the envelope of Spinach chloroplasts. J. biol. Chem. 248, 7215–7222 (1973)

    Google Scholar 

  • Folch, J., Lees, M., Sloane Stanley, G.H.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957)

    Google Scholar 

  • Gurr, M.I., Brawn, P.: The biosynthesis of polyunsaturated fatty acids by photosynthetic tissue. Europ. J. Biochem. 17, 19–22 (1970)

    Google Scholar 

  • Lechevallier, D., Bahl, J., Monéger, R.: Etude microanalytique des lipides dans de petits échantillons d'étioplastes et de chloroplastes isolés de feuilles de Blé. C. R. Acad. Sci. (Paris) 272, 963–966 (1972)

    Google Scholar 

  • Luft, J.H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Mackender, R.O., Leech, R.M.: Isolation and characterization of plastid envelope membranes. In: Proc. IInd Int. Congr. Photosynthetis Res. Stresa, Vol. 2, pp. 1431–1440, Forti G., Avron M., Melandri A., (ed.), The Hague: Dr. W. Junk NV 1972

    Google Scholar 

  • Mackender, R.O., Leech, R.: The galactolipid, phospholipid, and fatty acid composition of the chloroplast envelope membranes of Vicia faba L. Plant Physiol. 53, 496–502 (1974)

    Google Scholar 

  • Pohl, P., Glasl, H., Wagner, H.: Zur analytik pflanzlicher glyko und phospholipid und ihrer fettsäuren. I. Eine neue dünnschichtchromatographische methode zur trennung pflanzlicher lipoide und quantitativen bestimmung ihrer fettsäurezusammensetzung. J. Chromatog. 49, 488–49 (1970)

    Google Scholar 

  • Poincelot, R.: Isolation and lipid composition of Spinach chloroplast envelope membranes. Arch. Biochem. Biophys. 159, 134–142 (1973)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Wright, R. S.: A reagent for the non destructive location of steroids and some other lipophilic materials on silica gel thin-layer chromatograms. J. Chromatog. 59, 220–221 (1971)

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Régulations Métaboliques et Différenciation des Plastes, Institut de Biologie Végétale, Université Paris 6, Tour 53, 4 Place Jussieu, 75230, Paris Cedex 05, France

    Jacqueline Bahl, Bernadette Francke & René Monéger

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  1. Jacqueline Bahl
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  2. Bernadette Francke
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  3. René Monéger
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Bahl, J., Francke, B. & Monéger, R. Lipid composition of envelopes, prolamellar bodies and other plastid membranes in etiolated, green and greening wheat leaves. Planta 129, 193–201 (1976). https://doi.org/10.1007/BF00398257

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  • DOI: https://doi.org/10.1007/BF00398257

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