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Indications from photosynthetic components that iron is a limiting nutrient in primary producers on coral reefs

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Abstract

Because iron is not available generally in oxygenated sea water, it may be a limiting factor in marine primary production. This hypothesis was tested in the context of Davies Reef, Latitude 18°50′S (one of the coral reefs in the central region of the Great Barrier Reef system). Samples were collected for study in the period August, 1980 to March, 1981. Sea water around the reef contained ≦2x10-6 M Fe, surface sediments from the reef contained 66±26 (1 SD) ppm total Fe, and interstitial water near the surface contained ≧5x10-7 M Fe. Thus, Fe constituted a trace component of the reef environment, but limited Fe should be available to algae associated with the sediments. Specific biochemical analyses to test the Fe status of benthic photosynthetic organisms were carried out with a common blue-green alga, Phormidium sp., and a ubiquitous symbiotic dinoflagellate, Gymnodinium microadriaticum (zooxanthellae). The blue-green alga contained the electron transport protein, flavodoxin, which is found only in Fe-deficient organisms. Supporting evidence for Fe stress in this organism included chlorosis in the presence of plentiful biliprotein, and very low extractable photosynthetic cytochrome, c-553. The latter observations were shown to be the result of Fe deficiency in laboratory cultures of a blue-green alga, Synechococcus sp. These cultures showed that production of flavodoxin is not a universal response of algae to Fe stress, but that lowered cellular concentrations of Fe-containing proteins involved in photosynthesis probably is universal. The zooxanthellae from a soft coral, Sinularia sp., had three-fold lower total Fe and ferredoxin (an electron transport protein), than the same alga from a clam, Tridacna maxima. Thus, some algae in symbiotic associations may also suffer Fe-deficiency. It was concluded that the degree and extent of Fe-stress in primary producers on a coral reef may influence growth rates, biomass, and distribution of species.

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Author notes

  1. B. G. Hatcher

    Present address: Western Marine Regional Laboratories, C.S.I.R.O., P.O. Box 20, 6020, North Beach, Western Australia, Australia

Authors and Affiliations

  1. Australian Institute of Marine Science, P.M.B. No. 3, Mail Sorting Office, 4810, Townsville, Queensland, Australia

    B. Entsch, R. G. Sim & B. G. Hatcher

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Communicated by G. F. Humphrey, Sydney

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Entsch, B., Sim, R.G. & Hatcher, B.G. Indications from photosynthetic components that iron is a limiting nutrient in primary producers on coral reefs. Mar. Biol. 73, 17–30 (1983). https://doi.org/10.1007/BF00396281

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