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Cellular compartmentation of ureide biogenesis in root nodules of cowpea (Vigna unguiculata (L.) Walp.)

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Abstract

Cowpea (Vigna unguiculata (L.) Walp.) nodules have been investigated by means of cytochemical and immunocytochemical procedures at the ultrastructural level in order to assess the role of the uninfected cells in ureide biogenesis. Uricase activity in the nodules was shown by cytochemical methods to be localized exclusively in the numberous large peroxisomes confined to the uninfected cells; the small peroxisomes in the infected cells did not stain for uricase. Uricase was also localized in the peroxisomes of uninfected cells by immunogold techniques employing polyclonal antibodies against nodule-specific uricase of soybean. There was no labeling above background of any structures in the infected cells. The results indicate that the uninfected cells are essential for ureide biogenesis in cowpea. Although tubular endoplasmic reticulum, the presumptive site of allantoinase, increases greatly in the uninfected cells during nodule development, it virtually disappears as the nodules mature. The inconsistency between the disappearance of the tubular endoplasmic reticulum from older nodules and the high allantoinase activity reported for older plants remains to be explained.

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Abbreviations

DAB:

3,3-diaminobenzidine

ER:

endoplasmic reticulum

GARG:

goat anti-rabbit immunoglobulin G

IgG:

immunoglobulin G

kDa:

knodalton

Mr:

apparent molecular mass

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Department of Botany, University of Wisconsin, 33706, Madison, WI, USA

    Mary Alice Webb & E. H. Newcomb

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  1. Mary Alice Webb
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  2. E. H. Newcomb
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Webb, M.A., Newcomb, E.H. Cellular compartmentation of ureide biogenesis in root nodules of cowpea (Vigna unguiculata (L.) Walp.). Planta 172, 162–175 (1987). https://doi.org/10.1007/BF00394584

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  • DOI: https://doi.org/10.1007/BF00394584

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