Skip to main content
Log in

Variation in the lipid and fatty acid composition in purified membrane fractions from Sarcina aurantiaca in relation to growth phase

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

S. aurantiaca membrane lipid contains both branched and straight-chain fatty acids from C9 to C22 with the saturated branched C15 predominating in almost all of the lipid fractions studied. Unsaturated fatty acids are only present in low concentrations. Significant amounts of straight-chain, even-numbered acids, more common in gram-negative and gram-variable bacteria, are also present. All lipid fractions show a marked change in their fatty acid profiles from the exponential to stationary phase of growth. At least 88% of the total lipid is “free” lipid, and of this material, at least 62% is neutral lipid. The amount of the latter decreases, with a corresponding increase in phospholipid as cells go into stationary phase. During this time, there is a slight fall in the amount of glycolipid which contains predominately mannose, but also glucose and galactose.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Burchfield, H. P. and Storrs, E. E. 1962. Biochemical applications of gas chromatography, p. 549–554.—Academic Press, London.

    Google Scholar 

  • Croom, J. A. and McNeil, J. J. 1961. The long chain fatty acids of certain biotin-requiring bacteria.—Bacteriol. Proc. 1961: 170.

    Google Scholar 

  • Hunter, M. I. S. 1971. Structural aspects of the membrane and ultrastructural features of Sarcina flava and Sarcina morrhuae.—Ph. D. Thesis, St. Andrews University.

  • Hunter, M. I. S. and Thirkell, D. 1971. Variation in fatty acid composition of Sarcina flava membrane lipid with the age of the bacterial culture.—J. Gen. Microbiol. 65: 115–118.

    Google Scholar 

  • Huston, C. K. and Albro, P. W. 1964. Lipids of Sarcina lutea. I. Fatty acid composition of the extractable lipids.—J. Bacteriol. 88: 425–432.

    Google Scholar 

  • Joyce, G. H., Hammond, R. K. and White, D. C. 1970. Changes in membrane lipid composition in exponentially growing Staphylococcus aureus during the shift from 37° to 25°.—J. Bacteriol 104: 323–330.

    Google Scholar 

  • Kaneda, T. 1971. Factors affecting the relative ratio of fatty acids in Bacillus cereus.—Can. J. Microbiol. 17: 269–275.

    Google Scholar 

  • Kates, M. 1964. Bacterial lipids.—Adv. Lipid Res. 2: 17–90.

    Google Scholar 

  • Knivett, V. A. and Cullen, J. 1965. Some factors affecting cyclopropane acid formation in E. coli.—Biochem. J. 96: 771–776.

    Google Scholar 

  • Law, J. H., Zalkin, H. and Kaneshiro, T. 1963. Transmethylation reactions in bacterial lipids.—Biochim. Biophys. Acta 70: 143–151.

    Google Scholar 

  • Lennarz, W. J. and Talamo, B. 1966. The chemical characterization and enzymatic synthesis of mannolipids in Micrococcus lysodeikticus.—J. Biol. Chem. 241: 2707–2719.

    Google Scholar 

  • Macfarlane, M. G. 1961. Composition of lipid from protoplast membranes and whole cells of Micrococcus lysodeikticus.—Biochem. J. 79: 4p-5p.

    Google Scholar 

  • Morrison, S. J., Tornabene, T. G. and Kloos, W. E. 1971. Natural lipids in the study of relationships of members of the Family Micrococcaceae.—J. Bacteriol. 108: 353–358.

    Google Scholar 

  • O'Leary, W. M. 1967. The chemistry and metabolism of microbial lipids.—The World Publishing Co., Cleveland and New York.

    Google Scholar 

  • Salton, M. R. J. and Freer, J. H. 1965. Composition of the membranes isolated from several Gram-positive bacteria.—Biochim. Biophys. Acta 107: 531–538.

    Google Scholar 

  • Shaw, N. and Baddiley, J. 1968. Structure and distribution of glycosyl diglycerides in bacteria.—Nature 217: 142–144.

    Google Scholar 

  • Shockman, G. D., Kolb, J. J., Bakay, B., Conover, M. J. and Toennies, G. 1963. Protoplast membrane of Streptococcus faecalis.—J. Bacteriol. 85: 168–176.

    Google Scholar 

  • Sweeley, C. C., Bentley, R., Makita, M. and Wells, W. W. 1963. Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances.—J. Amer. Chem. Soc. 85: 2497–2507.

    Google Scholar 

  • Tornabene, T. G., Bennett, E. O. and Oró, J. 1967. Fatty acid and aliphatic hydrocarbon composition of Sarcina lutea grown in three different media.—J. Bacteriol. 94: 344–348.

    Google Scholar 

  • Veerkamp, J. H. 1971. Fatty acid composition of Bifidobacterium and Lactobacillus strains.—J. Bacteriol. 108: 861–867.

    Google Scholar 

  • Vorbeck, M. L. and Marinetti, G. V. 1965a. Intracellular distribution and characterisation of the lipids of Streptococcus faecalis (ATCC 9790).—Biochemistry 4: 296–305.

    Google Scholar 

  • Vorbeck, M. L. and Marinetti, G. V. 1965b. Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography.—J. Lipid Res. 6: 3–6.

    Google Scholar 

  • Wells, M. A. and Dittmer, J. C. 1963. The use of Sephadex for the removal of non-lipid contaminants from lipid extracts.—Biochemistry 2: 1259–1263.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thirkell, D., Gray, E.M.M. Variation in the lipid and fatty acid composition in purified membrane fractions from Sarcina aurantiaca in relation to growth phase. Antonie van Leeuwenhoek 40, 71–78 (1974). https://doi.org/10.1007/BF00394555

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00394555

Keywords

Navigation